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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (2): 132-134.doi: 10.3736/jcim20030214

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Study on estrogen receptor of adult human osteoblasts

Gui-lin Ouyang, Qing-ming Yang, Lian-fu Deng, Fu-ping Xu, Ya-ping Zhu   

  1. Department of Orthopaedics, Ruijin Hospital, Shanghai Second Medical University,Shanghai Institute of Traumatology and Orthopaedics, Shanghai 200025,China
  • Received:2003-05-28 Online:2003-06-20 Published:2018-10-19

Objective

To examine estrogen receptor (ER) in osteoblasts from adult human and to elucidate the mechanism of estrogen in modulating bone metabolism.


Methods

The cultured osteoblasts were harvested from bone chips by modified sequential digestive enzyme release and immunohistochemical assay of ER in osteoblasts were carried out in three groups of female adults: normal control (group 1), patients with moderate osteoporosis (group 2) and patients with serious osteoporosis (group 3). The percentages of ER-positive osteoblasts from the three groups were compared by t test.


Results

The brown marks that indicate ER were found in nuclei and plasma of the osteoblasts, and the percentages of ER-positive osteoblasts among three groups were significantly different.


Conclusion

ERs exist in nuclei and plasma of the osteoblasts. Estrogen may modulate bone metabolism through binding ER in nuclei and plasma of the osteoblasts. The reduction of ER of osteoblasts may play an important role in the pathogenesis of postmenopausal osteoporosis.

Key words: estrogen receptor, osteoblast, osteoporosis

CLC Number: 

  • R681-4

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组别 n 标记率(%)
I组正常组 6 97.17±1.27
II组骨质疏松组 6 85.83±1.47**
III组严重骨质疏松组 6 59.83±2.48△△
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