糖尿病高血脂所致大鼠肾组织SOD和GSH及MDA的变化

doi:10.3736/jcim20040114

Abstract

Objective: To study the changes of SOD, GSH and MDA in the renal tissues of diabetes rat models through the comparison of their metabolic features. Methods: Two types of diabetes rat models were established by feeding high-lipid food along with small dose injecting of streptozotocin (STZ) (model group 1) and repeated small dose injecting of STZ with complete Freund's adjuvant (CFA) (model group 2). And changes of the following were observed: body weight, urine volume, blood sugar, blood lipid, serum insulin, insulin sensitive index (ISI), superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) of the renal tissues. Results: The body weight of the two model groups of rats decreased, while the urine volume and the blood sugar increased predominantly. These indexes of the two model groups were significantly different from those of the normal group (P ＜0.05). The renal tissue MDA level increased while the SOD and GSH level decreased in both model group 1 and model group 2, but the changes in model group 1 were more obvious than those in the model group 2. The serum insulin level which decreased in model group 2 did not decrease significantly in model group 1, but the ISI in model group 1 decreased more significantly than that in model group 2 (P ＜0.05).Conclusion: The antioxidase activity decreased in renal tissues of diabetes rats and hyperlipidemia was the main cause of the corresponding oxidations.

Key words: diabetes mellitus, hyperlipidemia, superoxide dismutase, glutathione, malondialdehyde

CLC Number:

R587.24

He-xin Cao, Li-qun He, Ya-jing Shen. Changes of SOD, GSH and MDA in renal tissues of diabetic hyperlipidemia rats. Journal of Chinese Integrative Medicine, 2004, 2(1): 36-38.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20040114

http://www.jcimjournal.com/EN/Y2004/V2/I1/36

TrendMD

Figures/Tables 3

Tab 1

Tab 2

Tab 3

References 5

[1]	郭啸华, 刘志红, 李恒, 等.实验性2型糖尿病大鼠模型的建立[J].肾脏病与透析肾移植杂志,2000,9(4):351-355
[2]	李红, 徐蓉娟, 唐红, 等.转化生长因子β在早期糖尿病肾病大鼠血清中的含量及其意义[J].中华内分泌代谢杂志,2001,17(3):186-187
[3]	Proietto J, Filippis A, Nakhla C, et al.Nutrient-induced insulin resistance[J].Mol Cell Endocrinol,1999,151(1-2):143-149
[4]	赵明, 王晓, 王竹兰, 等.自由基代谢和磺脲类药物受体关系的初步研究[J].中国糖尿病杂志,2000,8(1):21-23 doi: 10.3321/j.issn:1006-6187.2000.01.006
[5]	陈宇, 郭晓蕙, 彭定琼, 等.血脂变化对OLETF糖尿病大鼠肾小球细胞外基质成分含量的影响[J].中国糖尿病杂志,2001,9(2):79-81

Related Articles 15

[1]	Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[2]	Olakunle Bamikole Afolabi, Omotade Ibidun Oloyede, Shadrack Oludare Agunbiade. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe²⁺-induced pancreatic oxidative stress. Journal of Integrative Medicine, 2018, 16(3): 192-198.
[3]	Chun-song Hu, Tengiz Tkebuchava. SEEDi^1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[4]	Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[5]	Jeremiah Olorunjuwon Olugbami, Robert Damoiseaux, Bryan France, Michael A. Gbadegesin, Adam Z. Stieg, Shivani Sharma, Oyeronke A. Odunola, James K. Gimzewski. Atomic force microscopy correlates antimetastatic potentials of HepG2 cell line with its redox/energy status: Effects of curcumin and Khaya senegalensis. Journal of Integrative Medicine, 2017, 15(3): 214-230.
[6]	Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[7]	Roslinah Mohamad Hussain, Noor Faradilla Abdullah, Zulkhairi Amom. Killing of Staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity. Journal of Integrative Medicine, 2016, 14(6): 456-464.
[8]	Yee Chi Peggy Wong. Need of integrated dietary therapy for persons with diabetes mellitus and “unhealthy” body constitution presentations. Journal of Integrative Medicine, 2016, 14(4): 255-268.
[9]	Ausanee Wanchai, Duangjai Phrompayak. Use of complementary and alternative medicine among Thai patients with type 2 diabetes mellitus. Journal of Integrative Medicine, 2016, 14(4): 297-305.
[10]	Sulaiman Al-Eidi, SalwaTayel, Fatima Al-Slail, Naseem Akhtar Qureshi, Imen Sohaibani, Mohamed Khalil, Abdullah Mohammad Al-Bedah. Knowledge, attitude and practice of patients with type 2 diabetes mellitus towards complementary and alternative medicine. Journal of Integrative Medicine, 2016, 14(3): 187-196.
[11]	Mohammad Hashem Hashempur, Mojtaba Heydari, Seyed Hamdollah Mosavat, Seyyed Taghi Heydari, Mesbah Shams. Complementary and alternative medicine use in Iranian patients with diabetes mellitus. Journal of Integrative Medicine, 2015, 13(5): 319-325.
[12]	Ahmad Ghorbani. Clinical and experimental studies on polyherbal formulations for diabetes: Current status and future prospective. Journal of Integrative Medicine, 2014, 12(4): 336-345.
[13]	Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng . Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[14]	Rajadurai Akilen, Zeller Pimlott, Amalia Tsiami, Nicola Robinson. The use of complementary and alternative medicine by individuals with features of metabolic syndrome. Journal of Integrative Medicine, 2014, 12(3): 171-174.
[15]	Cheng Huang. Natural modulators of liver X receptors. Journal of Integrative Medicine, 2014, 12(2): 76-85.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[2]	Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[3]	Yong Miao Edwin. Clinical critical qualitative evaluation of the selected randomized controlled trials in current acupuncture researches for low back pain. Journal of Chinese Integrative Medicine, 2010, 8(12): 1133-1146
[4]	Yong-mei Liu, Rui-hua Liu, Wen-jun Liu, Li Liu , Zhi-kui Wu, Yu-ying Chen. Effects of Chinese herbal medicine Yiqi Huoxue Formula on TGF-p/smad signal transduction pathway and connective tissue growth factor in rats with renal interstitial fibrosis. Journal of Chinese Integrative Medicine, 2010, 8(12): 1165-1173
[5]	Mei-jun Lü, Hui-yong Zhang, Zhe Zhang, Guan-lin Yang. Measurement of health-related quality of life in coronary heart disease: A review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1277-1285
[6]	Li-hui Dang, Xiao-nan Yang, Qing Xia. Protective effects of Chaiqin Chengqi Decoction on isolated pancreatic acinar cells in acute pancreatitis rats and the mechanisms. Journal of Chinese Integrative Medicine, 2008, 6(2): 176-179
[7]	Qin Zhang, Wen-tong Zhang, Jian-jun Wei, Xian-bo Wang, Ping Liu. Combined use of factor analysis and cluster analysis in classification of traditional Chinese medical syndromes in patients with posthepatitic cirrhosis. Journal of Chinese Integrative Medicine, 2005, 3(1): 14-18
[8]	Vamsi Reddy, Arvind Sridhar, Roberto F. Machado, Jiwang Chen. High sodium causes hypertension: Evidence from clinical trials and animal experiments. Journal of Integrative Medicine, 2015, 13(1): 1-8
[9]	Arthur de Sá Ferreira, Antonio Guilherme Pacheco. SimTCM: A human patient simulator with application to diagnostic accuracy studies of Chinese medicine. Journal of Integrative Medicine, 2015, 13(1): 9-19
[10]	Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114

Group	n	Urine volume (ml)	Body weight (g)	Blood glucose(mmol/L)
Normal group	10	10.57±0.98	311.43±15.47	6.37±0.51
Model group 1	7	42.57±12.34*	270.00±14.14*	23.93±6.82*
Model group 2	7	68.57±15.05*^△	167.14±9.51*^△	25.33±3.01*

Group	n	TC(mmol/L)	TG(mmol/L)	SOD（kU/L）	GSH（mmol/L）	MDA（μmol/L）
Normal group	10	1.56±0.21	0.40±0.07	362.57±25.01	3.78±1.38	18.50±5.71
Model group 1	7	2.11±0.58*	2.32±2.42*	200.87±50.39*	2.21±0.72*	63.84±32.28*
Model group 2	7	1.68±0.30	1.08±0.50	282.75±47.49*^△	2.60±0.56*	33.77±9.66^△

Group	n	Fasting blood glucose（mmol/L）	Serum insulin（mU/L）	ISI
Normal group	10	4.81±0.66	71.32±44.62	-5.61±0.86
Model group 1	7	6.36±1.49*	70.53±43.63	-5.95±0.51
Model group 2	7	6.37±0.87*	28.36±7.93*^△	-5.15±0.28^△

Changes of SOD, GSH and MDA in renal tissues of diabetic hyperlipidemia rats

RichHTML

PDF (PC)

Like