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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (3): 208-209.doi: 10.3736/jcim20040317

• Original Experimental Research • Previous Articles     Next Articles

Effect of melittin on apoptsis and necrosis of U2OS cells

Yong-qiang Chen1, Zhen-an Zhu1, Yong-qiang Hao1, Ke-rong Dai1, Chen Zhang2   

  1. 1. Department of Orthopaedics,Shanghai Ninth People’s Hospital, Shanghai Second Medical University, Shanghai 200011,China;
    2. Department of Traditional Chinese Medicine,Changhai Hospital, Second Military Medical University, Shanghai 200433,China
  • Online:2004-06-20 Published:2018-10-25

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Objective: To study the effect of melittin on apoptsis and necrosis of osteosarcoma cell line U2OS in vitro. Methods: Osteosarcoma cell line U2OS was treated with melittin. The growth and proliferation was observed by MTT assay and cell counting, and the necrosis was estimated by Trypan blue staining. The cell apoptsis, Fas and Apo2.7expression were detected by cytometer. Results: The data showed that melittin could inhibit the proliferation of U2OS dose-dependently at 16 and 64 mg/L. Cell apoptsis was detected by cytometer, when the cells were treated by 16 mg/L and 32 mg/L of melittin respectively, and the percentages of Fas and Apo2.7 positive cells were increased. Conclusion: Melittin inhibits the proliferation of osterosarcoma cell line through up-regulating Fas expression and inducing apoptsis.

Key words: melittin, osteosarcoma, apoptsis

CLC Number: 

  • R392.252

Tab 1

Inhibition rate of melittin or proliferation of osteosarcoma cell line U2OS after different treating time"

Concentration of
melittin (mg/L)		 Inhibition rate (%)
12 h 24 h
64 85.71 87.20
32 50.00 49.81
16 35.71 37.25
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[1] Chen Zhang, Bai Li, Shu-qin Lu, Yong Li, Yong-hua Su, Chang-quan Ling. Effects of melittin on expressions of mitochondria membrane protein 7A6, cell apoptosis-related gene products Fas and Fas Ligand in hepatocarcinoma cells. Journal of Chinese Integrative Medicine, 2007, 5(5): 559-563.
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