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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (4): 388-391.doi: 10.3736/jcim20060414

• Original Experimental Research • Previous Articles     Next Articles

Effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1

Tie Chong1, Jian-qiang Niu1, Zi-ming Wang1, Jun-jun She1, Chen Huang2   

  1. 1. Department of Urology, Second Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710004, China;
    2. Key Laboratory of Envi ronment and Genes Rela ted to Diseases , Xi’an Jiaotong Unive rsity , Minist ry of Education , Xi’an , Shaanxi Province 710004 , China
  • Online:2006-07-31 Published:2006-07-15

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Objective

To observe the effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1 in vitro, and to explore its mechanism.

Methods

The human renal cell carcinoma cell line GRC-1 was treated with matrine of different concentrations for 24, 48, 72 and 96 h respectively. The MTT assay was used to evaluate the cytotoxic effects of matrine on GRC-1 cells. The transmission electron microscope and flow cytometry were utilized to observe and detect the apoptosis of GRC-1 cells induced by matrine. The expression levels of Bcl-2 and Bax proteins were evaluated by streptavidin-biotin-peroxidase method.

Results

The matrine of different concentrations all have cytotoxic effects on GRC-1 cells, with obvious dose- and time-dependent effects. The apoptosis induced by matrine was confirmed in GRC-1 cells. With intervention of matrine (1.5g/L) for 12 h, the expression level of Bcl-2 in GRC-1 cells was decreased while the expression level of Bax was increased as compared with those in the untreated group.

Conclusion

The proliferation-inhibiting effects of matrine on human renal cell carcinoma cell line GRC-1 may be related to down-regulating the ratio of Bcl-2/Bax protein expression and promoting the apoptosis.

Key words: Matrine, Kidney neoplasms, Apoptosis

CLC Number: 

  • R737.11

Table 1

Growth-inhibiting effects of matrine on human renal cell carcinoma cell line GRC-1（ヌ±S, %）"

Concentration of matrine n Growth-inhibiting rate
24 h 48 h 72 h 96 h
0.3 g/L 5 6.3±5.7 8.8±3.2 14.9±12.8 18.4±12.0
0.5 g/L 5 11.5±6.6 12.3±9.6 21.9±6.5 22.4±2.0
0.8 g/L 5 20.5±7.7 23.1±8.1 25.4±9.0 36.2±4.8
1.0 g/L 5 23.6±6.1 25.4±7.4 29.2±9.5 45.8±2.9
1.5 g/L 5 31.6±4.2 32.0±5.5 34.0±4.9 60.2±2.1

Figure 1

Ultrastructure of human renal cell carcinoma cell line GRC-1 in different groups detected by transmission electron microscopeA: Untreated group; B: Matrine (1.0 g/L)-treated group (phase Ⅰ of apoptosis); C: Matrine (1.0 g/L)-treated group (phase Ⅱ of apoptosis)."

Figure 2

Classification percentage of human renal cell carcinoma cell line GRC-1 in different groups by AnnexinⅤ-FITC/PI staining assay and flow cytometryA: Untreated group; B: Matrine (0.5 g/L)-treated group; C: Matrine (1.0 g/L)-treated group; D: Matrine (1.5 g/L)-treated group."

Table 2

Expressions of Bcl-2 and Bax proteins in human renal cell carcinoma cell line GRC-1 in different groups（ヌ±S）"

Group n Bcl-2 protein Bax protein
Untreated 10 2.765±0.020 2.467±0.060
Matrine(1.5 g/L)-treated 10 2.655±0.005 2.699±0.020
t 12.84 10.82
P <0.01 <0.01
[1] Amato RJ . Renal cell carcinoma: review of novel single-agent therapeutics and combination regimens[J]. Ann Oncol, 2005,16(1):7-15
doi: 10.1093/annonc/mdi002 pmid: 15598929
[2] 司维柯, 李鹏, 王源 , 等. 苦参碱抗人肝癌细胞株HepG2的作用及其机制研究[J]. 国外医学·临床生物化学与检验学分册, 2003,24(5):288-290
doi: 10.3969/j.issn.1673-4130.2003.05.018
[3] 曾晖, 胡梅洁, 张曙 , 等. 苦参碱体外对胃癌细胞的杀伤作用[J]. 上海第二医科大学学报, 2004,24(1):38-40
[4] 彭彦辉, 郝玉宾, 史迎钦 , 等. 苦参碱对肠癌HT-29细胞株增殖的抑制作用及其机制[J]. 中华实验外科杂志, 2005,22(11):1353-1354
doi: 10.3760/j.issn:1001-9030.2005.11.028
[5] 赵亚群, 尚辉辉, 吴忠琪 , 等. 以顺铂为主方案加复方苦参注射液治疗晚期非小细胞肺癌[J]. 中国肺癌杂志, 2005,8(1):63-65
[6] 梁建新, 曾文铤, 朱科伦 , 等. 苦参碱联合介入化疗栓塞治疗中晚期肝癌疗效观察[J]. 广州医药, 2005,36(3):45-47
[7] 张德杰, 黄世林, 陈楠楠 , 等. 补骨脂素加长波紫外线照射对白血病K562细胞的杀伤作用[J]. 中西医结合学报, 2005,3(6):480-483
[8] Harrington DJ, Lessey BA, Rai V , et al. Tenascin is differentially expressed in endometrium and endometriosis[J]. J Pathol, 1999,187(2):242-248
[9] Oltvai ZN, Milliman CL, Korsmeyer SJ . Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death[J]. Cell, 1993,74(4):609-619
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