丹参多酚酸盐预防大鼠术后肠粘连的机制

doi:10.3736/jcim20070509

Abstract

Objective: To observe the effects of salvianolate on rats with postoperative intestinal adhesion and to explore the prevention mechanism.Methods: Forty SD male rats with intestinal adhesion were randomly divided into four groups: untreated group, low-dose salvianolate-treated group (12 mg/kg), medium-dose salvianolate-treated group (24 mg/kg) and high-dose salvianolate-treated group (48 mg/kg), with another ten SD male rats as normal control. Intraperitoneal injection of glucose was administered to the rats in the normal control group and the untreated group, and intraperitoneal injection of salvianolate was administered to the rats in the low-, medium- and high-dose salvianolate-treated groups. They were all treated for 8 days and once a day. On the eighth day after surgery the blood samples of each group were collected. Grades of intestinal adhesion were ranked by macroscopic observation. The adhesive tissues between viscera and belly wall were taken for pathological observation. The levels of interleukin-1β (IL-1β), interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) were determined by enzyme linked immunosorbent assay.Results: Salvianolate can significantly reduce the extent of postoperative intestinal adhesion, obviously decrease the levels of IL-1β, TNF-α and inhibit the hyperplasy of fibrous connective tissue. However, there was no significant impact on the level of IL-4.Conclusion: Salvianolate can reduce the extent of postoperative intestinal adhesion, decrease the expression of IL-1β and TNF-α and inhibit the hyperplasy of fibrous connective tissue. This may be the mechanism of salvianolate in preventing intestinal adhesion.

CLC Number:

R574

Xin-bin Sui, Qing Zhang, Hua-sen Qiu, Ji-chun Zhou, Xi-dong Gu, Zhou-xiang Lu, Chao Li, Gang Wang. Mechanism of salvianolate in preventing postoperative intestinal adhesion in rats. Journal of Chinese Integrative Medicine, 2007, 5(5): 521-525.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070509

http://www.jcimjournal.com/EN/Y2007/V5/I5/521

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Group	n	Grade of intestinal adhesion
Group	n	0	Ⅰ	Ⅱ	Ⅲ	Ⅳ
Normal control	10	8	2	0	0	0
Untreated*	10	0	1	0	7	2
Low-dose salvianolate-treated	10	3	2	1	2	2
Medium-dose salvianolate-treated^△	10	6	2	2	0	0
High-dose salvianolate-treated^△	10	7	3	0	0	0

Group	n	IL-1β	IL-4	TNF-α
Normal control group	10	166.59±37.29	335.07±64.13	60.45±21.95
Untreated group	10	216.66±28.46*	315.00±40.77	119.69±50.37*
Low-dose salvianolate-treated	10	212.68±25.14	331.57±65.12	75.91±47.27^△
Medium-dose salvianolate-treated	10	168.40±26.14^△	348.15±51.70	74.56±23.83^△
High-dose salvianolate-treated	10	166.73±37.94^△	355.36±47.16	69.51±39.00^△

Mechanism of salvianolate in preventing postoperative intestinal adhesion in rats

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