黄连解毒汤对2型糖尿病大鼠靶组织磷脂酰肌醇3激酶的影响

doi:10.3736/jcim20070513

Abstract

Objective: To observe the effects of Huanglian Jiedu Decoction (HLJDD), a traditional Chinese compound herbal medicine, on p85 mRNA and protein expressions of phosphatidylinositol-3-kinase (PI-3K) in target tissues (skeletal muscular and adipose tissues) in rats with type 2 diabetes mellitus (T2DM) and to investigate the molecular mechanism of HLJDD in treating T2DM. Methods: The male Wistar rats were injected with streptozotocin (STZ) 30 mg/kg through tail vein, and fed with high-fat and high-caloric diets to induce T2DM. Then the rats were randomly divided into untreated group, aspirin-treated group and HLJDD group, and treated correspondingly. Meanwhile, a group of normal animals without any treatment was set up for normal control group. Ten weeks later, serum fasting blood glucose (FBG), serum fasting insulin (FINS) and oral glucose tolerance test (OGTT) were routinely determined. The expressions of PI-3K p85 mRNA and protein in skeletal muscle and adipose tissue were determined with RT-PCR and Western blotting before and after insulin treatment.Results: Compared with the untreated group, the FBG and OGTT levels in T2DM rats treated with HLJDD decreased significantly (P＜0.05). The FINS in HLJDD group was lower than that in the normal control group (P＜0.05), but was not significantly different from that in the untreated group. The PI-3K p85 mRNA and protein expressions in HLJDD group obviously increased, as compared with those in the untreated group.Conclusion: The effect of HLJDD in treating T2DM was probably associated with its improvement of PI-3K p85 mRNA and protein expressions in skeletal muscle and adipose tissue of the T2DM rats.

Key words: Huanglian Jiedu Decoction, diabetes mellitus , type ２, phosphatidylinositol-３-kinase, rats

CLC Number:

R587.1

Dan Jin, Fu-er Lu, Guang Chen, Huan Sun, Xiao-hong Lu. Effects of Huanglian Jiedu Decoction on phosphatidylinositol-3-kinase expression in target tissues of type 2 diabetic rats. Journal of Chinese Integrative Medicine, 2007, 5(5): 541-545.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20070513

http://www.jcimjournal.com/EN/Y2007/V5/I5/541

TrendMD

Figures/Tables 5

Table 1

Figure 1

Figure 2

Figure 3

Figure 4

References 12

[1]	冷三华, 陆付耳, 屠庆年, 等.黄连解毒汤对2型糖尿病大鼠血糖和血脂代谢的影响[J].中国中医基础医学杂志,2003,9(4):43-45
	1Leng SH, Lu FE, Tu QN, et al.Effects of Huang Lian Jie Du Decoction on blood glucose and lipids metabolisms in type Ⅱ diabetic rats[J].Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi,2003,9(4):43-45
[2]	叶爱丽, 陆付耳, 徐丽君, 等.黄连解毒汤对胰岛素抵抗大鼠骨骼肌IRS-1表达及其酪氨酸磷酸化水平的影响[J].中医杂志,2005,46(9):695-697
	Ye AL, Lu FE, Xu LJ, et al.Effects of Huanglian Jiedu decoction on IRS-1 expression and tyrosine phosphorylation level in skeletal muscles of the rats with insulin resistance[J].Zhong Yi Za Zhi,2005,46(9):695-697
[3]	陈广, 陆付耳, 徐丽君.黄连解毒汤对2型糖尿病大鼠靶组织葡萄糖转运子4的影响[J].中西医结合学报,2007,5(4):412-415
	Chen G, Lu FE, Xu LJ.Effects of Huanglian Jiedu Decoction on glucose transporter 4 in target tissues of type 2 diabetic rats[J].Zhong Xi Yi Jie He Xue Bao,2007,5(4):412-415
[4]	吴洪元, 姜晓群, 陈贤琼.黄连解毒胶囊提取工艺研究[J].中成药,1998,20(11):1-3
	Wu HY, Jiang XQ, Chen XQ.Study on extraction process for Huanglianjiedu capsules[J].Zhong Cheng Yao,1998,20(11):1-3
[5]	5Yuan M, Konstantopoulos N, Lee J, et al.Reversal of obesity- and diet-induced insulin resistance with salicylates or targeted disruption of Ikkβ[J].Science,2001,293(5535):1673-1677
	Yuan M, Konstantopoulos N, Lee J, et al.Reversal of obesity- and diet-induced insulin resistance with salicylates or targeted disruption of Ikkβ[J].Science,2001,293(5535):1673-1677
[6]	孙敬方. 动物实验方法学[M].北京: 人民卫生出版社,2001137-139, 197-212
	Sun JF.Animal experiment technology[M].Beijing: People's Medical Publishing House,2001137-139,197-212
[7]	Tremblay F, Lavigne C, Jacques H, et al.Dietary cod protein restores insulin-induces activation of phosphatidylinositol 3-kinase/Akt and GLUT4 translocation to the T-tubules in skeletal muscle of high-fat-fed obese rats[J].Diabetes,2003,52(1):29-37
[8]	陆付耳, 王智民, 郭爱群.糖尿病从“毒”论治探讨[J].中国中医基础医学杂志,2002,8(5):15-17
	Lu FE, Wang ZM, Guo AQ.Exploration on the hypojournal of treating diabetes mellitus with the detoxification principle of traditional Chinese medicine[J].Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi,2002,8(5):15-17
[9]	Lee YS, Kim WS, Kim KH, et al.Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states[J].Diabetes,2006,55(8):2256-2264
[10]	Zhang CY, Parton LE, Ye CP, et al.Genipin inhibits UCP2-mediated proton leak and acutely reverses obesity-and high glucose-induced β cell dysfunction in isolated pancreatic islets[J].Cell Metab,2006,3(6):417-427 doi: 10.1016/j.cmet.2006.04.010
[11]	Jackson S, Bagstaff S, Lynn S, et al.Decreased insulin responsiveness of glucose uptake in cultured human skeletal muscle cells from insulin-resistant nondiabetic relatives of type 2 diabetic families[J].Diabetes,2000,49(7):1169-1177
[12]	Hundal RS, Petersen KF, Mayerson AB, et al.Mechanism by which high-dose aspirin improves glucose metabolism in type 2 diabetes[J].J Clin Invest,2002,109(10):1321-1326 doi: 10.1172/JCI14955 pmid: 12021247

Related Articles 15

[1]	Theocharis Koufakis, Kalliopi Kotsa, Nikolaos Papanas. Remission of type 2 diabetes depends on prompt comprehensive lifestyle changes upon diagnosis: How can this “Road to Damascus” experience be supported?. Journal of Integrative Medicine, 2022, 20(4): 288-291.
[2]	Yunita Sari, Akhyarul Anam, Annas Sumeru, Eman Sutrisna. The knowledge, attitude, practice and predictors of complementary and alternative medicine use among type 2 diabetes mellitus patients in Indonesia. Journal of Integrative Medicine, 2021, 19(4): 347-353.
[3]	Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[4]	Pukar Khanal, Basanagouda M. Patil. Integration of network and experimental pharmacology to decipher the antidiabetic action of Duranta repens L.. Journal of Integrative Medicine, 2021, 19(1): 66-77.
[5]	Baoyi Shao, Saiying Hou, Yuenyan Chan, Changchun Shao, Lixing Lao. Remission of new-onset type 2 diabetes mellitus in an adolescent using an integrative medicine approach: A case report. Journal of Integrative Medicine, 2021, 19(1): 85-88.
[6]	Abdulhakim Abubakar, Abdullahi Balarabe Nazifi, Idris Mohammed Maje, Yusuf Tanko, Joseph Akpojo Anuka, Ezzeldin Mukthar Abdurahman. Antihyperglycaemic activity of ethylacetate extract of Chlorophytum alismifolium in type 2 diabetes: The involvement of peroxisome proliferator-activated receptor-γ and dipeptidyl peptidase-4. Journal of Integrative Medicine, 2021, 19(1): 78-84.
[7]	Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[8]	Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[9]	Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[10]	Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[11]	Olakunle Bamikole Afolabi, Omotade Ibidun Oloyede, Shadrack Oludare Agunbiade. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe²⁺-induced pancreatic oxidative stress. Journal of Integrative Medicine, 2018, 16(3): 192-198.
[12]	Chun-song Hu, Tengiz Tkebuchava. SEEDi^1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[13]	Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[14]	Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[15]	Ausanee Wanchai, Duangjai Phrompayak. Use of complementary and alternative medicine among Thai patients with type 2 diabetes mellitus. Journal of Integrative Medicine, 2016, 14(4): 297-305.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2]	Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3]	Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4]	SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5]	Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6]	Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7]	Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8]	Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9]	Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10]	Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239

Group	n	FINS (mU/L)	FBG (mmol/L)	BG-0.5 h (mmol/L)	BG-1 h (mmol/L)	BG-2 h (mmol/L)
Normal control	12	33.31±3.86	3.39±0.19^△	5.91±0.22^△	6.84± 0.39^△	5.72±0.24^△
Untreated	12	25.41±1.65	5.13±1.32*	10.85±1.91*	14.17±3.00*	12.25±2.68*
ASP-treated	19	35.06±4.27	4.45±0.23	8.44±0.52*^△	9.59±0.61^△	8.22±0.41^△
HLJDD-treated	23	22.81±2.38*	3.70±0.12^△	8.79±0.33*	10.16±0.54*^△	8.95±0.48^△

Effects of Huanglian Jiedu Decoction on phosphatidylinositol-3-kinase expression in target tissues of type 2 diabetic rats

RichHTML

PDF (PC)

Cited

Like