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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 577-580.doi: 10.3736/jcim20070521

• Original Experimental Research • Previous Articles     Next Articles

Effect of isoflavones in regulating the transcription of target genes through estrogen receptors

Qing-shu Meng, Xiao-yan Zhu, Xiao-lu Tang, Bei Ma, Xin Ni   

  1. Department of Physiology , College of Basic Medical Science , Second Military Medical University , Shanghai 200433 , China
  • Online:2007-09-20 Published:2007-09-15

Objective: To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes.Methods: In this study, we used ERα or ERβ over-expressing Hela cells to observe the transcriptional regulation of genistein and daidzein on ERE reporter gene with calcium-phosphate method, and furthermore observing the effects of phytoestrogen antagonist ICI 182780 on their activation.Results: Our results showed that both genistein and daidzein could activate ERE receptor gene through ERα and ERβ, and these effects could be blocked by ICI 182780. Conclusion: Both genistein and daidzein can mimic estrogen's effect to activate the transcription of target genes through binding to the ERs.

Key words: isoflavones, genistein, daidzein, receptor, estrogen

CLC Number: 

  • R349.64

Figure 1

The activation on ERE reporter gene by genistein**P<0.01, vs control group; n=4."

Figure 2

The activation on ERE reporter gene by daidzein. **P<0.01, vs control group; n=4."

Figure 3

The transcriptional regulation of ICI182780 on activation induced by genistein and daidzeinA: Cells were cotransfected with ERα and ERE reporter gene. B: Cells were cotransfected with ERβ and ERE reporter gene. Ctr: control; GST: genistein; DDZ: daidzein; ICI: ICI 182780. *P<0.05, **P<0.01, vs control; △△P<0.01, vs genistein or daidzein; ▲▲P<0.01, vs ICI; n=4."

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