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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 577-580.doi: 10.3736/jcim20070521

• Original Experimental Research • Previous Articles     Next Articles

Effect of isoflavones in regulating the transcription of target genes through estrogen receptors

Qing-shu Meng, Xiao-yan Zhu, Xiao-lu Tang, Bei Ma, Xin Ni   

  1. Department of Physiology , College of Basic Medical Science , Second Military Medical University , Shanghai 200433 , China
  • Online:2007-09-20 Published:2007-09-15

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Objective: To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes.Methods: In this study, we used ERα or ERβ over-expressing Hela cells to observe the transcriptional regulation of genistein and daidzein on ERE reporter gene with calcium-phosphate method, and furthermore observing the effects of phytoestrogen antagonist ICI 182780 on their activation.Results: Our results showed that both genistein and daidzein could activate ERE receptor gene through ERα and ERβ, and these effects could be blocked by ICI 182780. Conclusion: Both genistein and daidzein can mimic estrogen's effect to activate the transcription of target genes through binding to the ERs.

Key words: isoflavones, genistein, daidzein, receptor, estrogen

CLC Number: 

  • R349．64

Figure 1

The activation on ERE reporter gene by genistein**P<0.01, vs control group; n=4."

Figure 2

The activation on ERE reporter gene by daidzein. **P<0.01, vs control group; n=4."

Figure 3

The transcriptional regulation of ICI182780 on activation induced by genistein and daidzeinA: Cells were cotransfected with ERα and ERE reporter gene. B: Cells were cotransfected with ERβ and ERE reporter gene. Ctr: control; GST: genistein; DDZ: daidzein; ICI: ICI 182780. *P<0.05, **P<0.01, vs control; △△P<0.01, vs genistein or daidzein; ▲▲P<0.01, vs ICI; n=4."

[1] Rossouw JE, Anderson GL, Prentice RL , et al. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results from the women's health initiative randomized controlled trial[J]. JAMA, 2002,288(3):321-333
[2] Kuiper GG, Lemmen JG, Carlsson B , et al. Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta[J]. Endocrinology, 1998,139(10):4252-4263
doi: 10.1210/endo.139.10.6216 pmid: 9751507
[3] Harris DM, Besselink E, Henning SM , et al. Phytoestrogens induce differential estrogen receptor alpha- or beta-mediated responses in transfected breast cancer cells[J]. Exp Biol Med, 2005,230(8):558-568
[4] Matsumura A, Ghosh A, Pope GS , et al. Comparative study of oestrogenic properties of eight phytoestrogens in MCF7 human breast cancer cells[J]. J Steroid Biochem Mol Biol, 2005,94(5):431-443
[5] Schreihofer DA . Transcriptional regulation by phytoestrogens in neuronal cell lines[J]. Mol Cell Endocrinol, 2005,231(1-2):13-22
doi: 10.1016/j.mce.2004.12.006 pmid: 15713532
[6] Kim J, Um SJ, Woo J , et al. Comparative effect of seeds of Rhynchosia volubilis and soybean on MG-63 human osteoblastic cell proliferation and estrogenicity[J]. Life Sci, 2005,78(1):30-40
[7] Routledge EJ, White R, Parker MG , et al. Differential effects of xenoestrogens on coactivator recruitment by estrogen receptor(ER) alpha and ERbeta[J]. J Biol Chem, 2000,275(46):35986-35993
[8] An J, Tzagarakis-Foster C, Scharschmidt TC , et al. Estrogen receptor beta-selective transcriptional activity and recruitment of coregulators by phytoestrogens[J]. J Biol Chem, 2001,276(21):17808-17814
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