白芍总苷辅助预防大鼠心脏移植排斥反应的研究

doi:10.3736/jcim20070522

Abstract

Objective: To evaluate the role of total glucosides of peony (TGP) as adjuvant therapy for prevention of cardiac allograft rejection in rats.Methods: Rats with cardiac allograft were randomly divided into control group, tacrolimus-treated group, TGP-treated group and tacrolimus plus TGP-treated group. Graft survival time was observed. Allografts in some cases were examined by histological study seven days after transplantation. At the same time, the levels of CD4+ and CD8+ T cell subsets in peripheral blood were examined by using flow cytometry; the hepatic function and renal function of recipients were also tested.Results: The graft survival time of the tacrolimus-treated group and tacrolimus plus TGP-treated group was (11.14±1.57) d and (13.57±1.99) d, respectively. The graft survival time of the tacrolimus plus TGP-treated group was longer than that of the tacrolimus-treated group (P<0.05). The histological study showed that the rejection of the tacrolimus plus TGP-treated group was slighter than that of the tacrolimus-treated group. The levels of CD4+ T cell subset in the peripheral blood of the tacrolimus-treated and tacrolimus plus TGP-treated groups were (38.71±5.15)% and (32.43±4.39)% respectively 7 days after transplantation. The level of CD4+ T cell subset in the tacrolimus plus TGP-treated group was lower than that in the tacrolimus-treated group (P<0.05). The level of CD8+ T cell subset and the hepatic and renal function had no significant differences between the tacrolimus-treated group and the tacrolimus plus TGP-treated group.Conclusion: Effects of tacrolimus plus TGP in prevention of rejection are better than tacrolimus monotherapy in rats with cardiac allograft and without increasing side effects.

CLC Number:

R654.28

SUN Yan, jun LI Dian, guang SHAN Shi. Total glucosides of peony as adjuvant therapy for prevention of cardiac allograft rejection in rats. Journal of Chinese Integrative Medicine, 2007, 5(5): 581-584.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20070522

http://www.jcimjournal.com/EN/Y2007/V5/I5/581

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Group	n	Survival time
Control	7	7.14±1.07
Tacrolimus-treated	7	11.14±1.57
TGP-treated	7	8.29±1.11
Tacrolimus plus TGP-treated	7	13.57±1.99*

Group	n	CD4+	CD8+
Control	7	39.86±5.87	23.43±4.16
Tacrolimus-treated	7	38.71±5.15	21.14±4.14
TGP-treated	7	38.43±3.45	22.43±4.35
Tacrolimus plus TGP-treated	7	32.43±4.39*	17.57±2.99

Group	n	ALT (U/L)	AST (U/L)	BUN (mmol/L)	Cr (μmol/L)
Control	7	62.57±12.95	232.14±81.98	6.46±1.28	23.43±5.62
Tacrolimus-treated	7	64.00±5.59	240.85±53.87	6.40±0.49	23.71±5.41
TGP-treated	7	64.43±6.99	231.00±38.80	6.57±0.74	24.71±4.27
Tacrolimus plus TGP-treated	7	59.43±5.91	238.29±62.73	6.43±0.58	24.86±5.55

Total glucosides of peony as adjuvant therapy for prevention of cardiac allograft rejection in rats

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