Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 577-580.doi: 10.3736/jcim20070521

• Original Experimental Research • Previous Articles     Next Articles

Effect of isoflavones in regulating the transcription of target genes through estrogen receptors

Qing-shu Meng, Xiao-yan Zhu, Xiao-lu Tang, Bei Ma, Xin Ni   

  1. Department of Physiology , College of Basic Medical Science , Second Military Medical University , Shanghai 200433 , China
  • Online:2007-09-20 Published:2007-09-15

Objective: To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes.Methods: In this study, we used ERα or ERβ over-expressing Hela cells to observe the transcriptional regulation of genistein and daidzein on ERE reporter gene with calcium-phosphate method, and furthermore observing the effects of phytoestrogen antagonist ICI 182780 on their activation.Results: Our results showed that both genistein and daidzein could activate ERE receptor gene through ERα and ERβ, and these effects could be blocked by ICI 182780. Conclusion: Both genistein and daidzein can mimic estrogen's effect to activate the transcription of target genes through binding to the ERs.

Key words: isoflavones, genistein, daidzein, receptor, estrogen

CLC Number: 

  • R349.64

Figure 1

The activation on ERE reporter gene by genistein**P<0.01, vs control group; n=4."

Figure 2

The activation on ERE reporter gene by daidzein. **P<0.01, vs control group; n=4."

Figure 3

The transcriptional regulation of ICI182780 on activation induced by genistein and daidzeinA: Cells were cotransfected with ERα and ERE reporter gene. B: Cells were cotransfected with ERβ and ERE reporter gene. Ctr: control; GST: genistein; DDZ: daidzein; ICI: ICI 182780. *P<0.05, **P<0.01, vs control; △△P<0.01, vs genistein or daidzein; ▲▲P<0.01, vs ICI; n=4."

[1] Rossouw JE, Anderson GL, Prentice RL , et al. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results from the women's health initiative randomized controlled trial[J]. JAMA, 2002,288(3):321-333
[2] Kuiper GG, Lemmen JG, Carlsson B , et al. Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta[J]. Endocrinology, 1998,139(10):4252-4263
doi: 10.1210/endo.139.10.6216 pmid: 9751507
[3] Harris DM, Besselink E, Henning SM , et al. Phytoestrogens induce differential estrogen receptor alpha- or beta-mediated responses in transfected breast cancer cells[J]. Exp Biol Med, 2005,230(8):558-568
[4] Matsumura A, Ghosh A, Pope GS , et al. Comparative study of oestrogenic properties of eight phytoestrogens in MCF7 human breast cancer cells[J]. J Steroid Biochem Mol Biol, 2005,94(5):431-443
[5] Schreihofer DA . Transcriptional regulation by phytoestrogens in neuronal cell lines[J]. Mol Cell Endocrinol, 2005,231(1-2):13-22
doi: 10.1016/j.mce.2004.12.006 pmid: 15713532
[6] Kim J, Um SJ, Woo J , et al. Comparative effect of seeds of Rhynchosia volubilis and soybean on MG-63 human osteoblastic cell proliferation and estrogenicity[J]. Life Sci, 2005,78(1):30-40
[7] Routledge EJ, White R, Parker MG , et al. Differential effects of xenoestrogens on coactivator recruitment by estrogen receptor(ER) alpha and ERbeta[J]. J Biol Chem, 2000,275(46):35986-35993
[8] An J, Tzagarakis-Foster C, Scharschmidt TC , et al. Estrogen receptor beta-selective transcriptional activity and recruitment of coregulators by phytoestrogens[J]. J Biol Chem, 2001,276(21):17808-17814
[1] Bérénice Colleville, Nicolas Perzo, Guillaume Avinée, Anaïs Dumesnil, Frederic Ziegler, Paul Billoir, Hélène Eltchaninoff, Vincent Richard, Eric Durand. Impact of high-fat diet and vitamin D3 supplementation on aortic stenosis establishment in waved-2 epidermal growth factor receptor mutant mice. Journal of Integrative Medicine, 2019, 17(2): 107-114.
[2] Ralph Fernando Rosas, Aline Armiliato Emer, Ana Paula Batisti, Daniela Dero Ludtke, Bruna Lenfers Turnes, Franciane Bobinski, Francisco José Cidral-Filho, Daniel Fernandes Martins. Far infrared-emitting ceramics decrease Freund’s adjuvant-induced inflammatory hyperalgesia in mice through cytokine modulation and activation of peripheral inhibitory neuroreceptors. Journal of Integrative Medicine, 2018, 16(6): 396-403.
[3] Yu-fan Shen, Xue Zhang, Ying Wang, Fan-fan Cao, Georges Uzan, Bin Peng, Deng-hai Zhang. Celastrol targets IRAKs to block Toll-like receptor 4-mediated nuclear factor-κB activation. Journal of Integrative Medicine, 2016, 14(3): 203-208.
[4] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[5] Zhong-liang Liu, Wei-rong Zhu, Wen-chao Zhou, Hai-feng Ying, Lan Zheng, Yuan-biao Guo, Jing-xian Chen, Xiao-heng Shen. Traditional Chinese medicinal herbs combined with epidermal growth factor receptor tyrosine kinase inhibitor for advanced non-small cell lung cancer: A systematic review and meta-analysis. Journal of Integrative Medicine, 2014, 12(4): 346-358.
[6] Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-Bukhsh​. Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: Drug-DNA interaction and signaling cascade. Journal of Integrative Medicine, 2014, 12(2): 102-114.
[7] Cheng Huang​. Natural modulators of liver X receptors. Journal of Integrative Medicine, 2014, 12(2): 76-85.
[8] Na Wu , Lu Song , Xin-xin Yang, Jiang-lei Wei , Zhen-guo Liu. Effects of Chinese herbal medicine Tianqi Pingchan Granule on G protein-coupled receptor kinase 6 involved in the prevention of levodopa-induced dyskinesia in rats with Parkinson disease. Journal of Chinese Integrative Medicine, 2012, 10(9): 1018-1024.
[9] Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[10] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
[11] Qing-lan Wang, Yan-yan Tao , Li Shen , Hong-yan Cui , Cheng-hai Liu. Chinese herbal medicine Fuzheng Huayu recipe inhibits liver fibrosis by mediating the transforming growth factor-β1/Smads signaling pathway. Journal of Chinese Integrative Medicine, 2012, 10(5): 561-568.
[12] De-bo Qi, Wei-min Li. Effects of electroacupuncture on expression of c-fos protein and N-methyl-D-aspartate receptor 1 in the rostral ventromedia medulla of rats with chronic visceral hyperalgesia. Journal of Chinese Integrative Medicine, 2012, 10(4): 416-423.
[13] Li-li Yang, Miao Wang, Tao Liu, Hai-yan Song, Dong-fei Li, Pei-yong Zheng, Ping Liu, Guang Ji. Effects of Chinese herbal medicine Jiangzhi Granule on expressions of liver X receptor α and sterol regulatory element-binding protein-1c in a rat model of non-alcoholic fatty liver disease. Journal of Chinese Integrative Medicine, 2011, 9(9): 998-1004.
[14] Qing-fang Ge, Hui-yun Zhang . Effects of Chinese herbal medicines for regulating liver qi on expression of 5-hydroxytryptamine 3B receptor in hypothalamic tissues of rats with anger emotion. Journal of Chinese Integrative Medicine, 2011, 9(8): 871-877.
[15] Ali Reza Ebrahimzadeh Bideskan, Mahmoud Hosseini, Toktam Mohammadpour, Reza Karami, Mehdi Khodamoradi, Habibollah Nemati Karimooy, Hassan Alavi. Effects of soy extract on pentylenetetrazol-induced seizures in ovariectomized rats. Journal of Chinese Integrative Medicine, 2011, 9(6): 611-618.
Full text



[1] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[6] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[7] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[8] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[9] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[10] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97