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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (6): 634-641.doi: 10.3736/jcim20070607

Special Issue: Traditional Chinese Medicine

• Original Experimental Research • Previous Articles     Next Articles

Establishment of Caco-2 cell monolayer model and standard operation procedure for assessing intestinal absorption of chemical components of traditional Chinese medicine

Xiu-wei Yang, Xiao-da Yang, Ying Wang, Lian Ma, Yue Zhang, Xiao-gai Yang, Kui Wang   

  1. State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100083,China
  • Online:2007-11-20 Published:2007-11-15

Objective: To establish Caco-2 (a human colon adenocarcinoma cell line) cell monolayer model and the standard operation procedure for studying and assessing intestinal absorption of chemical components of traditional Chinese medicine.Methods: Caco-2 cell monolayer model was established and evaluated by morphology feature using scanning electron microscope, inverted microscope and transepithelial electrical resistance (TEER) assay. Additionally, the model was further tested for the activity of alkaline phosphatase and the apparent permeability (Papp) of standard compounds, i.e. propranolol and atenolol, which were the control substances for high and poor transcellular transport marker, respectively.Results: The integrality of cell monolayer, cell differentiation (reflected by expression of alkaline phosphatase and cell monolayer morphology), and the Papp value of standard compounds in the established Caco-2 cell model were satisfactory. All parameters tested were in good agreement with those reported in the literature.Conclusion: The established Caco-2 cell model can be used to study the intestinal absorption of orally administrated chemical components of traditional Chinese medicine and their absorption mechanism.

Key words: Caco-2 cells, Chinese herbal drugs, intestinal absorption, biological transport

CLC Number: 

  • R348

Figure 1

Diagram of Transwell and transport experiment"

Figure 2

Apical side of Caco-2 monolayer (scanning electron micrograph)"

Figure 3

Characteristic microvilli of apical side of the differentiated Caco-2 monolayer (transmission electron micrograph)"

Figure 4

Tight junction-desmosome of Caco-2 monolayer (transmission electron micrograph)"

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