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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (1): 43-45.doi: 10.3736/jcim20050113

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of puerarin on radiation injury of experimental rats

Le-hong Jin,Chuan-fei Liu,Yu Zeng   

  1. Department of Physiology, School of Basic Medicine, Hangzhou Teachers College, Hangzhou, Zhejiang Province 310012, China
  • Received:2004-09-05 Online:2005-01-20 Published:2005-01-20
  • Contact: Chuan-fei Liu E-mail:chuanfeiliu@sohu.com

Objective

To observe the protective effects of puerarin on radiation injury of experimental rats and to discuss the possible mechanism of its radiation protection.

Methods

Wistar rats were divided randomly into 4 groups with 8 rats in each group: physiological saline non-radiation (SN) group, puerarin non-radiation (PN) group, physiological saline radiation (SR) group, puerarin radiation (PR) group. The source of radiation was cobalt-60 gamma rays, and the rats were exposed to radiation (1.2 Gy/min) at a dose of 18 Gy. Following irradiation of the rats, puerarin was injected intravenously at each dose of 30 mg/kg in 6 consecutive days (24 h interval). Samples were collected and assayed one week later.

Results

Puerarin delayed effectively the declines of the quantity of red blood cells and white blood cells in circulation due to ionizing irradiation, improved the index of thymus and spleen in ionizing irradiation rats, increased the activity of superoxide dismutase (SOD) and decreased the level of malondialdehyde (MDA) in the rat myocardial tissue.

Conclusion

Puerarin has a marked protective effect on ionizing irradiation injury of experimental rats, and its protective mechanism is probably based on oxidation-resistance.

Key words: Puerarin, Radiation, Ionizing, Blood cells, Immunity, Superoxide dismutase, Malondialdehyde, Animals, Laboratory

CLC Number: 

  • R285.5

Tab 1

Protective effect of puerarin on hemogram of radiated rats ($\bar{x}$±s)"

Group n RBC (×1012/L) WBC (×109/L)
SN 8 6.65±1.65 8.77±1.80
PN 8 6.40±1.60 8.90±1.75
SR 8 3.62±1.25 2.88±0.52
PR 8 5.22±1.35** 6.10±0.65**

Tab 2

Protective effect of puerarin on injury of spleen and thymus of radiated rats ($\bar{x}$±s)"

Group n Spleen weight/
body weight
Thymus weight/
body weight
SN 8 0.52±0.05 0.25±0.06
PN 8 0.50±0.03 0.27±0.05
SR 8 0.32±0.05 0.16±0.02
PR 8 0.46±0.07** 0.22±0.05**

Tab 3

Effect of puerarin on oxidation resistance of radiated rats ($\bar{x}$±s)"

Group n SOD
(U/mg protein)
MDA
(μmol/mg protein)
SN 8 276.6±58.5 17.6±2.6
PN 8 338.2±78.5 13.2±2.2
SR 8 136.6±50.8 35.3±3.3
PR 8 192.8±62.2** 22.8±3.4**
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