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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (11): 1048-1052.doi: 10.3736/jcim20101108

• Original Expreimental Research • Previous Articles     Next Articles

Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism

Bing Hu a,b,Hong-mei Anc,Ke-ping Shena, Qin Dua   

  1. a 5th Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b E-institute of Internal Medicine of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    c Department of Science and Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2010-02-23 Accepted:2010-07-13 Online:2010-11-20 Published:2010-11-15
  • Contact: Bing Hu E-mail:beearhu@hotmail.com

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Objective

Cell senescence is an important anti-cancer mechanism and may contribute to cancer therapeutic outcome. The present study observed the effects of Tenglong Buzhong Decoction (TLBZD), a Chinese herbal formula, on senescence in human colon carcinoma LS-174-T cells.

Methods

LS-174-T cells were treated with TLBZD, and morphology change was observed under a microscope. Cell senescence was identified by senescence-associated β-galactosidase (SA-β-gal) staining, and cell cycle was analyzed by flow cytometry. Expressions of p53, p21 WAF1/CIP1, p16 and RB and RB phosphorylation were detected by Western blot.

Results

After being treated with TLBZD, LS-174-T cells became enlarged and flattened by morphology; SA-β-gal staining was positive and cell cycle was arrested in G0/G1. In addition, up-regulations of p21 WAF1/CIP1 and p16, as well as inhibition of RB phosphorylation were detected in response to TLBZD treatment. Expressions of p53 and RB were unchanged after TLBZD treatment.

Conclusion

TLBZD is effective in inducing cell senescence in human colon carcinoma LS-174-T cells, which may relate to up-regulations of p21 WAF1/CIP1 and p16 and inhibition of RB phosphorylation.

Key words: Colonic neoplasms, Chinese herbal drugs, Cell senescence, Signal transduction

Figure 1

Effects of TLBZD on activities of SA-β-gal in LS-174-T cells and CRL-1790 cells (Light microscopy, ×200) A and B: LS-174-T cells; C and D: CRL-1790 cells; A and C: ddH2O; B and D: TLBZD."

Figure 2

Effects of TLBZD on cell cycle in LS-174-T cells"

Table 1

Effects of TLBZD on cell cycle in LS-174-T cells"

Figure 3

Effects of TLBZD on expressions of senescence-related genes in LS-174-T cells"

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