二仙汤及其拆方对雌性大鼠垂体前叶细胞黄体生成素和卵泡刺激素的影响

doi:10.3736/jcim20070613

Abstract

Objective: To investigate the effects of Erxian Decoction (EXD), a traditional Chinese compound herbal medicine and its separate prescriptions such as Wenshen Yijing Recipe (WSYJR, a recipe for warming kidney and replenishing essence), Ziyin Xiehuo Recipe (ZYXHR, a recipe for nourishing yin and dispersing fire) and Tiaoli Chongren Recipe (TLCRR, a recipe for regulating thoroughfare and conception vessels) and some extracts from EXD on the levels of LH and FSH in the primary cultured anterior pituitary cells from female rats.Methods: EXD, its separate prescriptions and traditional Chinese herbal extracts from EXD were added directly to the incubators. The levels of LH and FSH were tested by radioimmunoassay.Results: The levels of LH and FSH in the supernatant of anterior pituitary cells treated by EXD or its separate prescriptions including ZYXHR and TLCRR were increased significantly as compared with those in the blank control. There was a tendency for stimulating the secretions of LH and FSH in the WSYJR-treated group too. Dimethyl sulfoxide as the solvent of icariin and curculigoside could interfere with the results.Conclusion: EXD and its separate prescriptions such as ZYXHR and TLCRR can increase the levels of LH and FSH significantly.

Key words: pituitary gland, anterior, luteinizing hormone, follicle-stimulating hormone, rats

CLC Number:

R347.913

Bing-feng Dong, Jian-rong Shi, Zhao-qin Fang, Dong-yuan Guang, Qin Liu, Zhong-hua Wu. Effects of Erxian Decoction and its separate prescriptions on the levels of luteinizing hormone and follicle-stimulating hormone in anterior pituitary cells from female rats. Journal of Chinese Integrative Medicine, 2007, 5(6): 665-669.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20070613

http://www.jcimjournal.com/EN/Y2007/V5/I6/665

TrendMD

Figures/Tables 4

Table 1

Table 2

Table 3

Table 4

References 11

[1]	李荣群, 庄爱丈, 王猛 . 二仙汤的研究现状与思考[J]. 中国中医药信息杂志, 2004,11(6):553-555
	Li RQ, Zhuang AZ, Wang M . Research stasus quo and thingking on Erxian Decoction.[J]. Zhongguo Zhong Yi Yao Xin Xi Za Zhi, 2004,11(6):553-555
[2]	程士德 . 二仙汤的临床运用[J]. 山西中医, 1989,5(1):1-4
	Cheng SD . Clinical application of Erxian Decoction[J]. Shanxi Zhong Yi, 1989,5(1):1-4
[3]	方肇勤, 司富春, 张伯讷 , 等. 二仙汤及其拆方对老龄大鼠下丘脑GnRH基因转录与表达德调节作用[J]. 上海中医药大学上海市中医药研究院学报, 1998,12(1):59-61
	Fang ZQ, Si FC, Zhang BN , et al. Regulating function of two immortals decoction and its separated formulas in GnRH transcription and expression in hypothalamus of old rats[J]. Shanghai Zhong Yi Yao Da Xue Shanghai Shi Zhong Yi Yao Yan Jiu Yuan Xue Bao, 1998,12(1):59-61
[4]	方肇勤, 徐品初, 张伯讷 . 二仙汤及其拆方对老龄大鼠血浆性激素和促性腺激素含量的影响[J]. 中国医药学报, 1992,7(5):24-26
	Fang ZQ, Xu PC , Zhang BN.regulation and function of Erxian Decoction and its separate formulas on sex hormone and gonadotropins in old rats[J]. Zhongguo Yi Yao Xue Bao, 1992,7(5):24-26
[5]	胡玉珍, 张万会, 朱运龙 , 等. 免疫化学染色和放射自显影双标记技术在培养的大鼠垂体前叶细胞中的应用[J]. 中国应用生理学杂志, 1995,11(2):176-178
	Hu YZ, Zhang WH, Zhu YL , et al. The application of double-labeling method of immunocytochemistry and auto radiography to culture cells of rat anterior pituitary[J]. Zhongguo Ying Yong Sheng Li Xue Za Zhi, 1995,11(2):176-178
[6]	王高峰, 朱运龙, 胡玉珍 , 等. 白细胞介素对大鼠离体垂体前叶细胞增殖德影响[J]. 中国应用生理学杂志, 1997,13(1):64-67
	Wang GF, Zhu YL, Hu YZ , et al. Effort of interleukins on proliferation of cultured rat anterior pituitary cells[J]. Zhongguo Ying Yong Sheng Li Xue Za Zhi, 1997,13(1):64-67
[7]	李芳芳, 李恩, 吕占军 , 等. 淫羊霍苷对大鼠卵泡颗粒细胞和肾上腺皮质细胞分泌功能的影响[J]. 中国中药杂志, 1997,22(8):499-500
	Li FF, Li E, Lu ZJ , et al. Effort of icarrin on secretion function of cultured granulosa and adrenal coetical cells in rats[J]. Zhongguo Zhong Yao Za Zhi, 1997,22(8):499-500
[8]	赵万红, 曹永孝, 袁泽飞 . 中药血清药理学的方法学探讨[J]. 中药新药与临床药理, 2002,13(2):122-124
	Zhao WH, Cao YX, Yuan ZF . Discussion of serum pharmacology method in research of traditional chinese medicine[J]. Zhong Yao Xin Yao Yu Lin Chuang Yao Li, 2002,13(2):122-124
[9]	刘晴, 施建蓉, 杨颖 . 二仙汤及其拆方对大鼠性腺细胞分泌功能的影响[J]. 中国中药杂志, 2005,30(13):1023-1026
	Liu Q, Shi JR, Yang Y , et al. The effectson secretary function of rat gonad by Erxian Decoction ang its disassembled prescriptions[J]. Zhongguo Zhong Yao Za Zhi, 2005,30(13):1023-1026
[10]	葛志强, 李景川, 元英进 , 等. DMSO对悬浮培养的东北红豆杉细胞凋亡和紫杉醇合成的影响[J]. 植物生理学报, 2001,27(6):515-520
	Ge ZQ, Li JC, Yuan YJ , et al. Effect of DMSO on apoptosis and taxoi biosynjournal in suspension cultured taxus cuspidata cells[J]. Zhi Wu Sheng Li Xue Bao, 2001,27(6):515-520
[11]	丁伟璜, 阮小燕, 莫尚武 . 试谈中药复方药理研究的三个方法学问题[J]. 中国中医药信息杂志, 1999,6(6):8
	Ding WH, Ruan XY, Mo SW . Three methodological problems in the pharmacological research on Chinese herbal recipes[J]. Zhongguo Zhong Yi Yao Xin Xi Za Zhi, 1999,6(6):8

Related Articles 15

[1]	Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2]	Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3]	Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4]	Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5]	Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6]	Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7]	Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[8]	Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[9]	Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[10]	Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11]	Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[12]	Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[13]	Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[14]	Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng . Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[15]	Amir Rashidian, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2]	Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3]	Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4]	Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5]	. Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6]	Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7]	Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8]	Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9]	Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10]	Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228

Group	n	LH	FSH
Blank control	6	0.80±0.09	0.59±0.19
Erxian Decoction-treated	6	1.09±0.17*	1.35±0.22**
Wenshen Yijing Recipe-treated	6	0.94±0.17	0.71±0.24
Ziyin Xiehuo Recipe-treated	6	1.10±0.20*	1.38±0.07**
Tiaoli Chongren Recipe-treated	6	1.46±0.12**	1.38±0.20**

Group	n	LH	FSH
Blank control	6	0.88±0.35	0.48±0.52
DMSO control	6	0.41±0.05*	0.56±0.07*
Icariin-treated	6	0.81±0.56	0.47±0.01^△
Curculigoside-treated	6	0.71±0.15	0.50±0.07

Group	n	FSH
Blank control	6	0.59±0.19
Erxian Decoction-treated	6	1.35±0.22**
1/6 Erxian Decoction-treated	6	0.59±0.09
Wenshen Yijing Recipe-treated	6	0.71±0.24
1/3 Wenshen Yijing Recipe-treated	6	1.44±0.30**
Ziying Xiehuo Recipe-treated	6	1.38±0.07**
1/2 Ziying Xiehuo Recipe-treated	6	1.33±0.28**
Tiaoli Chongren Recipe-treated	6	1.38±0.20**

Group	n	FSH
Blank control	6	1.03±0.27
DMSO Control	6	1.36±0.60**
30 μg/L icariin-treated	6	0.73±0.14
100 μg/L icariin-treated	6	0.80±0.19
300 μg/L icariin-treated	6	0.93±0.19
1 000 μg/L icariin-treated	6	0.66±0.06

Effects of Erxian Decoction and its separate prescriptions on the levels of luteinizing hormone and follicle-stimulating hormone in anterior pituitary cells from female rats

RichHTML

PDF (PC)

Like