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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (2): 171-174.doi: 10.3736/jcim20080213

• Original Experimental Research • Previous Articles     Next Articles

Variabilities of the impacts of cold pathogen and cold-dampness pathogen on fractalkine mRNA expression in lung tissues of rats

Wei Zhang1(), Hai-yu Liu2   

  1. 1. Department of Respiratory Medicine, Affiliated Hospital, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province 250011, China
    2. Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province 250014, China
  • Received:2007-04-26 Online:2008-02-20 Published:2008-02-15
  • Contact: ZHANG Wei E-mail:05320835zh@sina.com

Objective:To study the variabilities of the effects of cold pathogen and cold-dampness pathogen on the fractalkine (FKN) mRNA expression in lung tissues of rats.

Methods:Twenty-four Wistar rats of SPF grade were randomly divided into 3 groups: normal temperature group, cold pathogen group and cold-dampness pathogen group. There were 8 rats in each group. Rats in normal temperature group were bred at (20±2)℃ and under normal humidity (50%-55%) for 2 h. Rats in cold pathogen group were bred at –10℃ and under normal humidity (50%-55%) for 2 h, and the rats in cold-dampness pathogen group were bred at –10℃ and under high humidity (90%-100%) for 2 h. Rats in the three groups were bred in thermostats under the corresponding conditions on the first day of experiment, and then the rats in different groups were all bred at normal temperature. Lung specimens in 3 groups were gathered four days later. The behavior and the pathological changes in the lung tissues of rats in different groups were observed. The content of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in lung homogenate was detected by radioimmunoassay (RIA) method. Expression of FKN mRNA in lung homogenate was detected by reverse transcription-polymerase chain reaction (RT-PCR).

Results:The lung tissues of rats in both cold pathogen group and cold-dampness pathogen group had various degrees of pathological changes. Compared with normal temperature group, the content of IL-6 and TNF-α was increased obviously in lung homogenate of rats in both cold pathogen group and cold-dampness pathogen group (P<0.01). The content of IL-6 and TNF-α in lung homogenate of rats in cold-dampness pathogen group was obviously higher than that in cold pathogen group (P<0.01). The RT-PCR results showed a low expression of FKN mRNA in lung tissues of rats in normal temperature group. If the injured lung tissues were aggravated, the expression of FKN mRNA in the lung tissues was elevated. Compared with normal temperature group, FKN mRNA expressions in both cold pathogen group and cold-dampness pathogen group were increased obviously (P<0.01). FKN mRNA expression in lung homogenate of rats in cold-dampness pathogen group was also obviously higher than that in cold pathogen group (P<0.01).

Conclusion:Cold pathogen can induce lung injury and up-regulate the FKN mRNA expression in lung tissue. Dampness pathogen can up-regulate the FKN mRNA expression through aggravating the injury of lung tissues caused by cold pathogen. FKN has a close relationship with the lung injury.

Key words: pathogenic cold, pathogenic dampness, lung, CX3C chemokines, rats

CLC Number: 

  • R563

Figure 1

Pathological characteristics of rat lung tissue in normal group (HE staining, ×200)"

Figure 2

Pathological characteristics of rat lung tissue in cold pathogen group (HE staining, ×200)"

Figure 3

Pathological characteristics of rat lung tissue in cold-dampness pathogen group (HE staining, ×200)"

Table 1

Changes of the content of IL-6 and TNF-α and the expression of FKN mRNA in lung tissues of rats in different groups ($\bar{x}$±s)"

Group n IL-6 (ng/L) TNF-α (ng/L) FKN mRNA
Normal temperature 8 42.54±2.08 113.00±8.41 0.674 3±0.060 8
Cold pathogen 8 69.86±1.38** 278.38±7.11** 1.354 4±0.115 0**
Cold-dampness pathogen 7 76.34±2.54**△△ 391.71±7.09**△△ 1.614 6±0.021 6**△△
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