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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 463-467.doi: 10.3736/jcim20080506

• Original Clinical Research • Previous Articles     Next Articles

Correlation between traditional Chinese medicine syndromes in primary immunoglobulin A nephropathy and A267G in 5'-untranslated region within exonal of megsin gene

Yi-fei Zhong1(), Yi-ping Chen1, Yue-yi Deng1, Nan Chen2, Zhao-hui Wang2   

  1. 1. Department of Nephrology, Longhua Hospital, Shanghai University of Tradtional Chinese Medicine, Shanghai 200032, China
    2. Department of Nephrology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Received:2008-01-17 Online:2008-05-20 Published:2008-05-15
  • Contact: ZHONG Yi-fei

Objective: To observe the correlation between traditional Chinese medicine (TCM) syndromes ("deficiency of qi and yin" and "deficiency of liver yin and kidney yin") and A267G in 5'-untranslated region within exonal of megsin gene, and to search the substantial genetic basis for micro-differentiation of TCM syndromes in primary immunoglobulin A nephropathy (IgAN).
Methods: A total of 120 IgAN cases meeting the diagnostic criteria were enrolled. The sequence of single nucleotide polymorphism (SNP) of A267G in 5'-untranslated region within exonal of megsin gene was tested. The correlation between SNP and TCM syndromes was observed.
Results: There were 83 cases carrying GG genotype, 34 cases carrying GA genotype and 3 cases carrying AA genotype in 120 cases of primary IgAN. There was a high proportion of "deficiency of liver yin and kidney yin" in IgAN cases with AA and GA genotypes, and a high proportion of "deficiency of qi and yin" in IgAN cases with GG genotype (P<0.01). Odds ratio in TCM syndrome distribution between GG genotype and GA plus AA genotype was 9.800, and 95% confidence interval was 3.969-24.199. The discrepancy also resided in IgAN patients with different genders and ages.
Conclusion: A267G in 5'-untranslated region within exonal of the megsin gene may be one of the substantial genetic basis for differentiating "deficiency of liver yin and kidney yin" syndrome and "deficiency of qi and yin" syndrome in primary IgAN.

Key words: immunoglobulin A nephropathy, polymorphism, single nucleotide, gene, megsin

CLC Number: 

  • R692.9

Figure 1

650 bp PCR production in different patients A 650 bp fragment was detected in nine IgAN patients' DNA. The DNA ladder sizes were shown on the left."

Figure 2

The sequence wave of megsin gene (Reverse) A: GG genotype; B: AA genotype; C: GA genotype."

Table 1

Distribution differences of polymorphism of A267G in 5'-untranslated region within exonal of megsin gene in different traditional Chinese medicine syndromes"

Syndrome type n Polymorphism of A267G in 5'-untranslated region within exonal of the megsin gene Total
Deficiency of qi and yin 72 9 (12.50%) 0 (0.00%) 63 (87.50%) 72 (100%)
Deficiency of liver yin and kidney yin 48 25 (52.08%) 3 (6.25%) 20 (41.67%) 48 (100%)
Total 120 34 (28.33%) 3 (2.50%) 83 (69.17%) 120 (100%)

Table 2

Distribution differences of polymorphism of A267G in 5’-untranslated region within exonal of megsin gene in traditional Chinese medicine syndromes of different sexes"

Sex Syndrome type Polymorphism of A267G in 5'-untranslatedregion within exonal of the megsin gene Total
Deficiency of qi and yin 28 (87.50%) 4 (12.50%) 0 (0.00%) 32 (100%)
Deficiency of liver yin and kidney yin 10 (45.45%) 11 (50.00%) 1 (4.55%) 22 (100%)
Total 38 (70.37%) 15 (27.78%) 1 (1.85%) 54 (100%)
Deficiency of qi and yin 35 (87.50%) 5 (12.50%) 0 (0.00%) 40 (100%)
Deficiency of liver yin and kidney yin 10 (38.46%) 14 (53.85%) 2 (7.69%) 26 (100%)
Total 45 (68.18%) 19 (28.79%) 2 (3.03%) 66 (100%)

Table 3

Distribution differences of polymorphism of A267G in 5'-untranslated region within exonal of megsin gene in traditional Chinese medicine syndromes of different ages"

Age Syndrome type Polymorphism of A267G in 5'-untranslated region within exonal of the megsin gene Total
<35 years
Deficiency of qi and yin 31 (86.11%) 5 (13.89%) 0 (0.00%) 36 (100%)
Deficiency of liver yin and kidney yin 9 (42.86%) 10 (47.62%) 2 (9.52%) 21 (100%)
Total 40 (70.18%) 15 (26.32%) 2 (3.50%) 57 (100%)
≥35 years
Deficiency of qi and yin 32 (88.89%) 4 (11.11%) 0 (0.00%) 36 (100%)
Deficiency of liver yin and kidney yin 11 (40.74%) 15 (55.56%) 1 (3.70%) 27 (100%)
Total 43 (68.25%) 19 (30.16%) 1 (1.59%) 63 (100%)
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