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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (6): 621-625.doi: 10.3736/jcim20080615

• Original Experimental Research • Previous Articles     Next Articles

Bushen Huoxue Huayu Recipe up-regulates expression of insulin receptor substrate-1 and phosphorylation of tyrosine in adipose tissue in androgen sterilized rats

Xi-lian Li1, Zhen-zhi Wang2, Chao-qin Yu2()   

  1. 1. Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    2. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2008-01-24 Online:2008-06-20 Published:2008-06-15
  • Contact: YU Chao-qin E-mail:chqyu81@sina.com

Objective: To investigate the expression of insulin receptor substrate-1 (IRS-1) and phosphorylation of tyrosine in androgen sterilized rats (ASRs) and the effects of Bushen Huoxue Huayu Recipe (BHHR), a compound Chinese herbal medicine for tonifying kidney and activating blood, on the ASRs, and to analyze the possible mechanism.
Methods: ASR models were established by means of testosterone injected subcutaneously at the age of 9 days. The model rats were randomly divided into BHHR group (n=15) and untreated group (n=15); the rats in normal group (n=15) were injected with oil. The rats in the BHHR group were treated with BHHR for 30 days, and the rats in model group and normal group were treated with distilled water for 30 days.Body weight and area under the curve (AUC) of insulin were measured after treatment. Expression of IRS-1 and phosphorylation of tyrosine in adipose tissue in ASRs were analysed by immunohistochemical method.
Results: The body weight and insulin AUC in the untreated group were increased and more than those in the BHHR group and the normal control group (P<0.05). Expression of IRS-1 and phosphorylation of tyrosine in normal group were significantly higher than those in the untreated group (P<0.05), and those in BHHR group were improved and significantly different from those in the untreated group (P<0.05).
Conclusion: BHHR can increase the expression of IRS-1 and phosphorylation of tyrosine in adipose tissue in IR rats, which may be one of its mechanisms in improving insulin sensitivity of the target tissues.

Key words: polycystic ovary syndrome, insulin resistance, insulin receptor substrate-1, tyrosine, phosphorylation, rats

CLC Number: 

  • R271.14

Table 1

AUC of insulin and body weight of rats in three groups ($\bar{x}$±s)"

Group n Body weight (g) AUC of insulin (mg/L)
Normal control 15 226.14±7.30 0.168 6±0.030 4
Untreated 15 252.93±14.78* 0.311 1±0.100 3*
BHHR 15 240.20±18.80 0.246 7±0.082 0*

Table 2

Expression of IRS-1 and phosphorylation of tyrosine in adipose tissues of rats in three groups ($\bar{x}$±s)"

Group n IRS-1 Phosphorylation of tyrosine
Normal control 15 0.293 8±0.036 4 0.283 6±0.037 8
Untreated 15 0.259 2±0.045 8* 0.209 2±0.045 8*
BHHR 15 0.287 2±0.030 6* 0.279 6±0.030 2*

Figure 1

Expression of IRS-1 in adipose tissues of rats in three groups (Immunohistochemical staining,×100) A: Normal control group; B: Untreated group; C: BHHR group."

Figure 2

Degree of phosphorylation of tyrosine in adipose tissues of rats in three groups (Immunohistochemical staining,×100) A: Normal control group; B: Untreated group; C: BHHR group."

[1] Dunaif A, Wu X, Lee A , et al. Defects in insulin receptor signaling in vivo in the polycystic ovary syndrome(PCOS)[J]. Am J Physiol Endocrinol Metab, 2001,281(2):E392-E399
doi: 10.1152/ajpendo.2001.281.2.E392 pmid: 11440917
[2] Wu JH, Yu CQ, Zhou QL , et al. Clinical study on effect of Bushen Huayu Qutan Recipe in treating polycystic ovarian syndrome[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007,27(10):883-886
吴建辉, 俞超芹, 周巧玲 , 等. 补肾化瘀祛痰方治疗多囊卵巢综合征的临床研究[J]. 中国中西药结合杂志, 2007,27(10):883-886
[3] Wang L, Yu J . Effect of Tiangui Recipe on hypothalamic proopiomelanocortin mRNA levels in alldrogen sterilized rat[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2001,21(7):519-521
doi: 10.7661/CJIM.2001.7.519
王莉, 俞瑾 . 中药天癸方对雄激素致不孕大鼠下丘脑前阿黑皮原mRNA的影响[J]. 中国中西医结合杂志, 2001,21(7):519-521
doi: 10.7661/CJIM.2001.7.519
[4] 王针织, 俞超芹 . 补肾化瘀祛痰方对雄激素致不孕大鼠卵巢局部调控因子的影响[J]. 中西医结合学报, 2008,6(4):361-365
Wang ZZ, Yu CQ . Effects of Bushen Huayu Qutan Recipe on local ovarian factors in androgen-sterilized rats[J]. Zhong Xi Yi Jie He Xue Bao, 2008,6(4):361-365
[5] Burghen GA, Givens JR, Kitabchi AE . Correlation of hyperandrogenism with hyperinsulinism in polycystic ovarian disease[J]. J Clin Endocrin Metab, 1980,50(1):113-116
doi: 10.1210/jcem-50-1-113
[6] Marsden PJ, Murdoch AP, Taylor R . Tissue insulin sensitivity and body weight in polycystic ovary syndrome[J]. Clin Endocrinol (Oxf), 2001,55(2):191-199
doi: 10.1046/j.1365-2265.2001.01303.x pmid: 11531925
[7] Holte J . Polycystic ovary syndrome and insulin resistance: thrifty genes struggling with over-feeding and sedentary life style?[J]. J Endocrinol Invest, 1998,21(9):589-601
doi: 10.1007/BF03350784
[8] Nestler JE, Jakubowicz DJ . Lean women with polycystic ovary syndrome respond to insulin reduction with decreases in ovarian P450c17 alpha activity and serum androgens[J]. J Clin Endocrinol Metab, 1997,82(12):4075-4079
[9] Pessin JE, Saltiel AR . Signaling pathways in insulin action: molecular targets of insulin resistance[J]. J Clin Invest, 2000,106(2):165-169
doi: 10.1172/JCI10582 pmid: 10903329
[10] Wiedmann M, Tamaki S, Silberman R , et al. Constitutive over-expression of the insulin receptor substrate-1 causes functional up-regulation of Fas receptor[J]. J Hepatol, 2003,38(6):803-810
doi: 10.1016/S0168-8278(03)00117-X
[11] Chu YL, Sun YY, Qiu HY , et al. Tyrosine phosphorylation and protein expression of insulin receptor substrate-1 in the patients with polycystice ovary syndrome[J]. Zhonghua Fu Chan Ke Za Zhi, 2004,39(3):176-179
初永丽, 孙永玉, 邱红玉 , 等. 胰岛素受体底物1蛋白表达及其酪氨酸磷酸化程度与多囊卵巢综合征发病的关系[J]. 中华妇产科杂志, 2004,39(3):176-179
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