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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (6): 626-631.doi: 10.3736/jcim20080616

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xiehuo Bushen Decoction on survival and differentiation of transplanted neural stem cells in brains of rats with intracerebral hemorrhage

Peng-cheng Zhong, Xing-qun Li(), Qing-hua Liang, Wen-fang Luo, Qing-er Liu, Jie-kun Luo, Tao Tang, Yuan Lin   

  1. Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China
  • Received:2007-09-29 Online:2008-06-20 Published:2008-06-15
  • Contact: LI Xing-qun E-mail:lixingqun7213@163.com

Objective: To investigate the effects of Xiehuo Bushen Decoction (XHBSD), a compound Chinese herbal medicine, on the survival and differentiation of transplanted neural stem cells (NSCs) in brains of rats with intracerebral hemorrhage, and to explore the mechanism of Xiehuo Bushen formula in promoting the survival of transplanted NSCs.
Methods: NSCs separated from hippocampuses of neonatal SD rats were cultured. Sixty-five panel reactive antibody (PRA) positive SD rats were selected by lymphocytotoxicity methods. The PRA positive rats were made into intracerebral hemorrhagic model and divided into three groups: cerebral hemorrhage group (n=15), NSCs transplanted group (n=25) and XHBSD group (n=25). XHBSD was orally administered after 5-bromodeoxyuridine (BrdU)-marked NSCs were transplanted in brains of rats with intracerebral hemorrhage in the XHBSD group. Rats in the other two groups were administered distilled water. The expressions of interferon gamma (IFN-γ) and interleukin-4 (IL-4) mRNAs were measured by reverse transcription polymerase chain reaction (RT-PCR); the numbers of BrdU and 200 kD neurofilament (NF200) positive cells were detected by double-labeling immunofluorescence method.
Results: The expression of IFN-γ mRNA was down-regulated significantly in the XHBSD group, but the expression of IL-4 mRNA was up-regulated significantly (P<0.05). The numbers of BrdU and NF200 positive cells were also increased remarkably in the XHBSD group.
Conclusion: XHBSD can promote the survival and differentiation of transplanted NSCs, which may be related to inducing the expression of IL-4 mRNA and inhibiting the expression of IFN-γ mRNA.

Key words: Xiehuo Bushen Decoction, neural stem cells, interferon gamma, interleukin-4, rats

CLC Number: 

  • R289.5

Figure 1

Nestin positive NSCs (Immunofluorescence staining, ×400)"

Figure 2

BrdU positive NSCs (Immunofluorescence staining, ×400)"

Figure 3

Caesar red staining positive lymphocytes (Inverted microscope, ×100) PRA value is about 80%. "

Figure 4

Expression of IFN-γ mRNA in XHBSD group at different time points"

Figure 5

Expression of IL-4 mRNA in XHBSD group at different time points"

Table 1

Expressions of IFN-γ and IL-4 mRNAs 2, 4 and 7 days after transplantation ($\bar{x}$±s)"

Group n IFN-γ mRNA IL-4 mRNA
2 days 4 days 7 days 2 days 4 days 7 days
Cerebral hemorrhage 15 0.73±0.01 0.65±0.01 0.63±0.03 0.91±0.12 0.92±0.02 0.94±0.13
NSCs transplanted 25 0.80±0.21* 0.67±0.02 0.65±0.12* 0.90±0.02* 0.89±0.11 0.90±0.05*
XHBSD 25 0.68±0.01** 0.63±0.01 0.59±0.01** 0.96±0.02 0.97±0.03 0.99±0.01*

Figure 6

BrdU and NF200 positive cells in XHBSD group and NSCs transplanted group 21 d after transplantation (Double immunofluorescent labeled staining, ×400) A: XHBSD group; B: NSCs transplanted group"

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