Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 852-855.doi: 10.3736/jcim20080815

• Original Experimental Research • Previous Articles     Next Articles

Effects of psoralen plus long-wave ultraviolet-A on apoptosis of HL-60 leukemia cells

Nan-nan Chen(), Shi-lin Huang, Yang Xiang, Li Zhang, De-jie Zhang   

  1. Department of Traditional Chinese Medicine and Hematology, Dalian 210th Hospital of People's Liberation Army, Dalian, Liaoning Province 116021, China
  • Received:2007-12-11 Online:2008-08-20 Published:2008-08-15

Objective: To explore the effects of psoralen (PSO) plus long-wave ultraviolet-A (PUVA) on apoptosis and expression of Fas ligand (FasL) in HL-60 leukemia cells.
Methods: The HL-60 cells were taken as the study objects and their apoptosis rates, ultrastructure changes and the expression of FasL were detected in order to observe the effects of PSO and ultraviolet-A (UVA) of wave length 360 nm. The factorial design and analysis of variance were used to analyze the interaction among the factors.
Results: PSO, UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than those of the other two. After HL-60 cells had been treated with PUVA, they all showed obvious ultrastructure changes due to apoptosis observed under the electron microscope. PSO, UVA and PUVA all decreased the expressions of FasL gene and protein. The effects of PUVA were stronger than those of the other two.
Conclusions: PUVA can induce the apoptosis of HL-60 cells and the effects are stronger than those of PSO or UVA alone. The expression of FasL gene in HL-60 cells is down-regulated during the apoptosis induced by PUVA.

Key words: leukemia, HL-60 cells, apoptosis, Fas ligand, ultraviolet therapy, photochemotherapy, psoralen

CLC Number: 

  • R733.71

Table 1

Apoptotic percentages of HL-60 cells and expressions of FasL mRNA and protein in HL-60 cells after 24-hour PUVA treatment (x±s)"

Group n Apoptosis ratio (%) FasL mRNA (bp/μg) FasL protein (%)
HL-60 cell control 3 10.60±0.31 (8.69±0.44)×107 58.67±1.75
UVA-treated 3 28.53±0.05** (2.64±0.31)×107** 33.90±1.90**
10 μg/ml PSO-treated 3 18.90±0.06** (7.03±0.47)×107** 58.40±1.87**
20 μg/ml PSO-treated 3 25.64±0.23** (5.68±0.47)×107** 57.57±1.48**
40 μg/ml PSO-treated 3 26.19±1.21** (5.17±0.38)×107** 54.33±1.69**
80 μg/ml PSO-treated 3 26.94±0.26** (2.71±0.29)×107** 37.40±1.73**
PUVA (10 μg/ml)-treated 3 29.17±0.38**△△ (1.58±0.38)×107**△△ 19.13±1.31**△△
PUVA (20 μg/ml)-treated 3 32.56±0.04**△△ (8.20±1.45)×106**△△ 18.50±1.51**△△
PUVA (40 μg/ml)-treated 3 33.39±0.11**△△ (1.64±0.40)×106**△△ 17.53±1.74**△△
PUVA (80 μg/ml)-treated 3 37.72±0.09**△△ (2.61±0.33)×105**△△ 15.60±1.42**△△

Figure 1

Change of ultrastructure in HL-60 cells after 24-hour PUVA treatment A: TEM, ×2 500; B: TEM, ×6 000."

[1] Chen NN, Huang SL, Zhang DJ , et al. The effects of psoralen and ultraviolet A on NB4, HL-60 and K562 leukemia cell lines[J]. Zhongguo Zhong Yi Ji Zheng, 2007,16(4):444-446
doi: 10.3969/j.issn.1004-745X.2007.04.036
陈楠楠, 黄世林, 张德杰 , 等. 补骨脂素加长波紫外线对人白血病细胞株NB4、HL-60、K562作用的研究[J]. 中国中医急症, 2007,16(4):444-446
doi: 10.3969/j.issn.1004-745X.2007.04.036
[2] Wu SH, Zhang ZH, Zhao JB . Antitumor activity of psoralen on mammary cancer cell line EMT 6 in vitro and in vivo[J]. Zhongguo Zhong Yao Za Zhi, 1998,23(5):303-305
吴少华, 张仲海, 赵建斌 . 补骨脂素体内外抗癌活性的实验研究[J]. 中国中药杂志, 1998,23(5):303-305
[3] Carneiro Leite V, Ferreira Santos R, Chen Chen L , et al. Psoralen derivatives and longwave ultraviolet irradiation are active in vitro against human melanoma cell line[J]. J Photochem Photobiol B, 2004,76(1-3):49-53
doi: 10.1016/j.jphotobiol.2004.07.004
[4] Plumas J, Drillat P, Jacob MC , et al. Extracorporeal photochemotherapy for treatment of clonal T cell proliferations[J]. Bull Cancer, 2003,90(8-9):763-770
doi: 10.1038/sj.bjc.6601084 pmid: 14609767
[5] Efferth T, Fabry U, Osieka R . Induction of apoptosis, depletion of glutathione, and DNA damage by extracorporeal photochemotherapy and psoralen with exposure to UV light in vitro[J]. Anticancer Res, 2001,21(4A):2777-2783
[6] Oginsky EL, Green GS, Griffith DG , et al. Lethal photosensitization of bacteria with 8-methoxypsoralen to long wave length ultraviolet radiation[J]. J Bacteriol, 1959,78(6):821-833
[7] Nagata S, Golstein P . The Fas death factor[J]. Science, 1995,267(5203):1449-1456
doi: 10.1126/science.7533326
[1] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[2] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[3] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[4] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
[5] Bruno José Martins Da Silva, Sandro Wilson Gomes Pereira, Ana Paula Drummond Rodrigues, José Luiz Martins Do Nascimento, Edilene Oliveira Silva. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 2018, 16(6): 404-410.
[6] Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[7] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[8] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[9] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[10] Samrat Ghosh, Sourav Sikdar, Avinaba Mukherjee, Anisur Rahman Khuda-Bukhsh. Evaluation of chemopreventive potentials of ethanolic extract of Ruta graveolens against A375 skin melanoma cells in vitro and induced skin cancer in mice in vivo. Journal of Integrative Medicine, 2015, 13(1): 34-44.
[11] Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-Bukhsh​. Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: Drug-DNA interaction and signaling cascade. Journal of Integrative Medicine, 2014, 12(2): 102-114.
[12] Paul Avijit, Das Sreemanti, Das Jayeeta, Samadder Asmita, Bishayee Kausik, Sadhukhan Ratan, Anisur Rahman Khuda-Bukhsh. Diarylheptanoid-myricanone isolated from ethanolic extract of Myrica cerifera shows anticancer effects on HeLa and PC3 cell lines: Signalling pathway and drug-DNA interaction. Journal of Chinese Integrative Medicine, 2013, 11(6): 405-415.
[13] Farha A. Kabeer, Geetha B. Sreedevi, Mangalam S. Nair, Dhanya S. Rajalekshmi, Latha P. Gopalakrishnan, Sujathan Kunjuraman, Remani Prathapan. Antineoplastic effects of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, on lung adenocarcinoma (A549) cells. Journal of Chinese Integrative Medicine, 2013, 11(4): 269-277.
[14] Samrat Ghosh, Kausik Bishayee, Avijit Paul, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Homeopathic mother tincture of Phytolacca decandra induces apoptosis in skin melanoma cells by activating caspase-mediated signaling via reactive oxygen species elevation. Journal of Chinese Integrative Medicine, 2013, 11(2): 116-124.
[15] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[2] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[8] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[9] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[10] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035