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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 852-855.doi: 10.3736/jcim20080815

• Original Experimental Research • Previous Articles     Next Articles

Effects of psoralen plus long-wave ultraviolet-A on apoptosis of HL-60 leukemia cells

Nan-nan Chen(), Shi-lin Huang, Yang Xiang, Li Zhang, De-jie Zhang   

  1. Department of Traditional Chinese Medicine and Hematology, Dalian 210th Hospital of People's Liberation Army, Dalian, Liaoning Province 116021, China
  • Received:2007-12-11 Online:2008-08-20 Published:2008-08-15

Objective: To explore the effects of psoralen (PSO) plus long-wave ultraviolet-A (PUVA) on apoptosis and expression of Fas ligand (FasL) in HL-60 leukemia cells.
Methods: The HL-60 cells were taken as the study objects and their apoptosis rates, ultrastructure changes and the expression of FasL were detected in order to observe the effects of PSO and ultraviolet-A (UVA) of wave length 360 nm. The factorial design and analysis of variance were used to analyze the interaction among the factors.
Results: PSO, UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than those of the other two. After HL-60 cells had been treated with PUVA, they all showed obvious ultrastructure changes due to apoptosis observed under the electron microscope. PSO, UVA and PUVA all decreased the expressions of FasL gene and protein. The effects of PUVA were stronger than those of the other two.
Conclusions: PUVA can induce the apoptosis of HL-60 cells and the effects are stronger than those of PSO or UVA alone. The expression of FasL gene in HL-60 cells is down-regulated during the apoptosis induced by PUVA.

Key words: leukemia, HL-60 cells, apoptosis, Fas ligand, ultraviolet therapy, photochemotherapy, psoralen

CLC Number: 

  • R733.71

Table 1

Apoptotic percentages of HL-60 cells and expressions of FasL mRNA and protein in HL-60 cells after 24-hour PUVA treatment (x±s)"

Group n Apoptosis ratio (%) FasL mRNA (bp/μg) FasL protein (%)
HL-60 cell control 3 10.60±0.31 (8.69±0.44)×107 58.67±1.75
UVA-treated 3 28.53±0.05** (2.64±0.31)×107** 33.90±1.90**
10 μg/ml PSO-treated 3 18.90±0.06** (7.03±0.47)×107** 58.40±1.87**
20 μg/ml PSO-treated 3 25.64±0.23** (5.68±0.47)×107** 57.57±1.48**
40 μg/ml PSO-treated 3 26.19±1.21** (5.17±0.38)×107** 54.33±1.69**
80 μg/ml PSO-treated 3 26.94±0.26** (2.71±0.29)×107** 37.40±1.73**
PUVA (10 μg/ml)-treated 3 29.17±0.38**△△ (1.58±0.38)×107**△△ 19.13±1.31**△△
PUVA (20 μg/ml)-treated 3 32.56±0.04**△△ (8.20±1.45)×106**△△ 18.50±1.51**△△
PUVA (40 μg/ml)-treated 3 33.39±0.11**△△ (1.64±0.40)×106**△△ 17.53±1.74**△△
PUVA (80 μg/ml)-treated 3 37.72±0.09**△△ (2.61±0.33)×105**△△ 15.60±1.42**△△

Figure 1

Change of ultrastructure in HL-60 cells after 24-hour PUVA treatment A: TEM, ×2 500; B: TEM, ×6 000."

[1] Chen NN, Huang SL, Zhang DJ , et al. The effects of psoralen and ultraviolet A on NB4, HL-60 and K562 leukemia cell lines[J]. Zhongguo Zhong Yi Ji Zheng, 2007,16(4):444-446
doi: 10.3969/j.issn.1004-745X.2007.04.036
陈楠楠, 黄世林, 张德杰 , 等. 补骨脂素加长波紫外线对人白血病细胞株NB4、HL-60、K562作用的研究[J]. 中国中医急症, 2007,16(4):444-446
doi: 10.3969/j.issn.1004-745X.2007.04.036
[2] Wu SH, Zhang ZH, Zhao JB . Antitumor activity of psoralen on mammary cancer cell line EMT 6 in vitro and in vivo[J]. Zhongguo Zhong Yao Za Zhi, 1998,23(5):303-305
吴少华, 张仲海, 赵建斌 . 补骨脂素体内外抗癌活性的实验研究[J]. 中国中药杂志, 1998,23(5):303-305
[3] Carneiro Leite V, Ferreira Santos R, Chen Chen L , et al. Psoralen derivatives and longwave ultraviolet irradiation are active in vitro against human melanoma cell line[J]. J Photochem Photobiol B, 2004,76(1-3):49-53
doi: 10.1016/j.jphotobiol.2004.07.004
[4] Plumas J, Drillat P, Jacob MC , et al. Extracorporeal photochemotherapy for treatment of clonal T cell proliferations[J]. Bull Cancer, 2003,90(8-9):763-770
doi: 10.1038/sj.bjc.6601084 pmid: 14609767
[5] Efferth T, Fabry U, Osieka R . Induction of apoptosis, depletion of glutathione, and DNA damage by extracorporeal photochemotherapy and psoralen with exposure to UV light in vitro[J]. Anticancer Res, 2001,21(4A):2777-2783
[6] Oginsky EL, Green GS, Griffith DG , et al. Lethal photosensitization of bacteria with 8-methoxypsoralen to long wave length ultraviolet radiation[J]. J Bacteriol, 1959,78(6):821-833
[7] Nagata S, Golstein P . The Fas death factor[J]. Science, 1995,267(5203):1449-1456
doi: 10.1126/science.7533326
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