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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (11): 1170-1173.doi: 10.3736/jcim20081112

• Original Experimental Research • Previous Articles     Next Articles

Possible role of GABAA-benzodiazepine receptor in anticonvulsant effects of Pasipay in rats

Nassiri-Asl Marjan1(), Zamansolt Farzaneh2Shariati-R Schwann3   

  1. 1. Department of Pharmacology, School of Medical Sciences, Qazvin University, Qazvin, Iran
    2. Department of Anatomy, School of Medical Sciences, Qazvin University, Qazvin, Iran
    3. School of Medical Sciences, Qazvin University, Qazvin, Iran
  • Received:2008-04-20 Online:2008-11-20 Published:2008-11-15


To investigate the anticonvulsant effects of Pasipay, a commercially available preparation of hydro-alcoholic extract of Passiflora incarnata in rats.


The anticonvulsant effects of hydro-alcoholic extract of P. incarnata, Pasipay, were observed by intracerebroventricular injection of 0.125, 0.25, 0.55 and 1.5 μg Pasipay.


Pasipay could dose-dependently affected minimal clonic seizures and generalized tonic-clonic seizures induced by pentylenetetrazole, through increment in seizure onset significantly. Additionally, pretreatment with 5 nmol/L flumazenil could abolish the anticonvulsant effects of Pasipay on the onset of both seizures.


The results indicate that Pasipay has anticonvulsant effects in the brain, possibly through positive allosteric modulation of the GABAA receptor complex via interaction at the benzodiazepine site.

Key words: Passiflora incarnata, anticonvulsants, diazepam, GABAa receptor, rats

CLC Number: 

  • R971.6


Group MCS (n) MCS latency (x±s, s) GTCS (n) GTCS latency (x±s, s) Survival rate (%)
Vehicle 10 50.4±2.3 10 81.3±10.1 0
10 μmol/L Diazepam 8 268.0±69.7** 4 447.4±67.4** 60
0.125 μg Pasipay 10 49.5±1.8 10 179.0±43.7 0
0.25 μg Pasipay 10 62.0±3.9 10 225.8±84.4 0
0.55 μg Pasipay 10 110.0±32.4 7 386.7±59.2** 30
1.5 μg Pasipay 8 256.2±86.4** 6 395.8±101.7** 40
5 nmol/L flumazenil 10 50.5±4.5 10 85.5±17.1 0
Flumazenil plus diazepam 10 63.0±3.9 10 133.8±16.3 0
Pasipay plus flumazenil 10 47.5±4.4 10 150.3±18.9 0
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