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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (1): 53-58.doi: 10.3736/jcim20090108

• Original Experimental Research • Previous Articles     Next Articles

Mechanism of Yiqi Huayu Bushen Recipe in treating cervical syndrome in rats with qi deficiency, blood stasis and kidney deficiency

 Yong-jun Wang, Qi Shi,  Jian-chun Jiang,  Qin Bian,  Qian-qian Liang, Chen-guang Li,  Quan Zhou,  Xue-jun Cui,  Sheng Lu,  Chong-jian Zhou   

  1. Institute of Spine Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-05-30 Accepted:2008-07-24 Online:2009-01-20 Published:2009-01-15


To explore the mechanism of Yiqi Huayu Bushen Recipe (YHBR), a compound traditional Chinese herbal medicine, in treating cervical syndrome (CS) with qi deficiency, blood stasis and kidney deficiency in rats.

A total of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal control group, untreated group and YHBR group; there were ten rats in each group. The rat model of cervical syndrome with qi deficiency, blood stasis and kidney deficiency was established by combining disease and syndrome models. After one-month YHBR treatment in YHBR group, all rats were sacrificed. Serum, plasma and cervical intervertebral discs were detected and observed by radio-immunology, histopathology, immunohistochemistry, etc.

Compared with those in the normal control group, rats in the untreated group showed obvious signs of deficiency in vital energy, such as tiredness, ptosis, few movement, bluish-purple tongue and tail and weight loss; the weight of uterus and appendages, and the ratio of cyclic adenosine monophosphate/cyclic guanosine monophosphate were decreased; hemorheological parameters and the expression of alpha-granular membrane protein (CD62p) were increased; the content of serum estradiol was decreased. YHBR could improve the above indexes except for the weight of uterus and appendages. HE staining showed annulus fibrosus with cracks, diminish of pulposus nucleus, and decrease in the height of intervertebral disc and the thickness of end-plate in the untreated group; and YHBR could improve the changes of cervical intervertebral discs, but no obvious changes in end-plate. Type Ⅱ collagen protein (Col2a1)was distributed in every part of the annulus fibrosus in normal control group, but was decreased in untreated group; YHBR could increase Col2a1 expression in annulus fibrosus as compared with the untreated group. The expressions of Col2a1 and tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNAs were decreased in untreated group as compared with the normal control group (P<0.01); the expressions of Col2a1 and TIMP-1 mRNAs were higher and matrix metalloproteinase-13 mRNA was lower in the YHBR group than those in the untreated group (P<0.05, P<0.01).

YHBR may improve the condition of cervical syndrome with qi deficiency, blood stasis and kidney deficiency by regulating immune system, coagulation system and endocrine system, and delay the degeneration of cervical intervertebral disc by regulating extracellular matrix and metalloproteases in intervertebral disc.

Key words: Yiqi Huayu Bushen Recipe, Qi deficiency, Blood stasis, Kidney deficiency, Cervical syndrome, Rats

Table 1

Contents of plasma cAMP, cGMP and ratio of cAMP/cGMP in three groups (x±s)"

Group n cAMP (nmol/L) cGMP (nmol/L) cAMP/cGMP
Normal control 7 36.78±10.66 5.21±2.08 8.09±4.35
Untreated 7 37.34±12.81 4.39±2.62 9.28±2.08
YHBR 7 42.39±12.96 2.65±1.10* 18.86±10.18*

Table 2

Changes of hemorheological parameters in three groups (x±s, mPa·s)"

Group n Whole blood
low-shear viscosity
Whole blood
high-shear viscosity
Reducing blood
Aggregation index
Normal control 10 8.59±0.76 3.96±0.16 16.49±2.07 2.17±0.17
Untreated 8 11.32±1.20** 5.37±0.51** 23.08±1.96** 2.24±0.11
YHBR 10 10.60±1.17** 4.90±0.22**△△ 22.47±1.86** 2.16±0.17

Table 3

Expression of CD62p in three groups (x±s, %)"

Group n CD62p
Normal control 8 5.20±3.42
Untreated 8 42.97±4.05**
YHBR 8 33.60±6.44**

Table 4

Content of serum E2 in three groups (x±s, μg/L)"

Group n E2
Normal control 8 4.85±0.86
Untreated 7 2.14±0.41**
YHBR 8 3.13±1.13**

Figure 1

HE staining of cervical intervertebral disc (Light microscopy, ×40) A: Normal control group; B: Untreated group; C: YHBR group."

Figure 2

Immunohistochemical staining of type Ⅱ collagen in annulus fibrosus of cervical intervertebral disc (Light microscopy, ×200) A: Normal control group; B: Untreated group; C: YHBR group."

Table 5

Expressions of Agc1, Col2a1, MMP-13 and TIMP-1 mRNAs in cervical intervertebral disc(x±s)"

Group n Agc1/β-actin Col2a1/β-actin MMP-13/β-actin TIMP-1/β-actin
Normal control 6 1.00±0.34 1.00±0.48 1.00±0.84 1.00±0.46
Untreated 6 1.03±0.05 0.12±0.03** 1.87±0.44 0.48±0.07**
YHBR 6 1.33±0.32 0.81±0.20 0.36±0.19 1.12±0.07△△
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