复方浙贝颗粒联合阿霉素对K562/A02移植瘤mdr1基因表达的影响

doi:10.3736/jcim20090810

Abstract

Objective

To investigate the effects of Compound Zhebei Granule (CZBG), a compound traditional Chinese herbal medicine, combined with adriamycin (ADM) on mdr₁ gene expression in tumor xenografts in nude mice.

Methods

A tumor xenografts model was established by subcutaneously injecting multidrug resistance cell line K562/A02 into the axillary flank of BALB/c-nu mice. Sixty tumor-bearing nude mice were divided into untreated group, ADM group and high-, medium-, and low-dose CZBG plus ADM groups. The nude mice in the CZBG plus ADM groups were intragastrically administered with CZBG once per day and intraperitoneally injected with ADM once every other day. The nude mice in the untreated group and the ADM group were administered with normal saline or ADM respectively. The course of treatment was continuous for 14 days. The weight of tumor xenografts was measured after treatment and the inhibition rate of tumor xenografts was calculated. The mdr₁gene expression in tumor xenografts was tested by quantitative polymerase chain reaction method.

Results

Compared with the untreated group, CZBG (high-, medium- and low-dose) plus ADM could significantly decrease the weight of tumor xenografts in nude mice (P<0.05). Compared with ADM, high- and medium-dose CZBG plus ADM could decrease the weight of tumor xenografts in nude mice with statistical significance (P<0.05). The high- and medium-dose CZBG plus ADM could also significantly decrease the mdr1 gene expression in tumor xenografts as compared with ADM alone (P<0.05).

Conclusion

CZBG at high- and medium-dose plus ADM can inhibit the growth of tumor xenografts and decrease the mdr₁ gene expression in tumor xenografts in nude mice.

Key words: Compound Zhebei Granule, Multidrug resistance, K562/A02 cell line, Tumor xenograft, Mdr₁, Mice, Nude

Zhi Zheng , Ju Chen, Dong-yun Li, Xin-yi Chen. Effects of Compound Zhebei Granule plus doxorubicin on mdr1 gene expression in nude mice with K562/A02 tumor xenografts. Journal of Chinese Integrative Medicine, 2009, 7(8): 758-514.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090810

http://www.jcimjournal.com/EN/Y2009/V7/I8/758

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References 13

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Group	n	Tumor weight ($\bar{x}$±s, g)	IR (%)
Untreated	9	1.413±0.676	0
ADM	9	1.000±0.589	28.66
Low-dose CZBG plus ADM	10	1.057±0.613	25.18
Medium-dose CZBG plus ADM	9	0.728±0.428^△	48.42
High-dose CZBG plus ADM	9	0.418±0.295^△	70.35

Group	n	Ct (mdr₁)	Ct (abl)	ΔCt (Ctmdr₁–Ctabl)	ΔΔCt (ΔCt–ΔCtcontrol)	Normalized 2–ΔΔCt
Untreated	5	20.60±1.07	27.24±1.07	–6.64±0.24	–0.03±0.23	1.03±0.17
ADM	5	21.22±1.07	28.00±0.62	–6.78±0.47	–0.17±0.47	1.17±0.36
Low-dose CZBG plus ADM	5	20.17±1.75	27.15±1.34	–6.98±0.41	–0.37±0.41	1.32±0.40
Medium-dose CZBG plus ADM	5	20.43±1.39	26.11±0.67	–5.67±1.00	0.93±0.39	0.64±0.10*^△
High-dose CZBG plus ADM	5	19.78±1.82	26.46±1.85	–6.68±0.33	–0.075±0.33	1.07±0.26^△
Calibrator model	5	21.77	28.38	–6.61	0	1

Effects of Compound Zhebei Granule plus doxorubicin on mdr1 gene expression in nude mice with K562/A02 tumor xenografts

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