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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (11): 1067-1072.doi: 10.3736/jcim20091109

• Original Experimental Research • Previous Articles     Next Articles

Effects of No.2 Renal Failure Recipe on expressions of cyclooxygenase-2 and -1 mRNAs in rats with chronic renal failure

Guan-jun Pu, Chen Wang, Ping-dong Zheng, Li-qun He   

  1. Department of Nephrology of Shuguang Hospital, Key Laboratory of Liver and Kidney Diseases of Ministry of Education, E-institute of Shanghai Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
  • Received:2009-07-06 Accepted:2009-08-11 Online:2009-11-20 Published:2009-11-15
  • Contact: Chen Wang


To observe the effects of No.2 Renal Failure Recipe (No.2RFR), a compound traditional Chinese herbal medicine, on expressions of cyclooxygenase-2 (COX-2) and cyclooxygenase-1 (COX-1) mRNAs in rats with chronic renal failure (CRF).

A rat model of CRF was successfully established by infarction of approximately two-thirds of the left kidney and removal of the right kidney (ablation/infarction, A/I). Thirty A/I rats were randomly divided into untreated group, celebrex group and No.2RFR group. Another 10 SD rats were selected as normal control group. After 2-month treatment, the pathology of the nephridial tissue was observed with hematoxylin and eosin straining under a light microscope. Renal function including serum creatinine (SCr) and blood urea nitrogen (BUN) was determined by using an automatic biochemical analyzer. Expressions of COX-2 and COX-1 mRNAs in nephridial tissues were detected by reverse transcription-polymerase chain reaction (RT-PCR).

No.2RFR could significantly decrease the levels of SCr and BUN. Renal function and morphology of CRF rats were ameliorated and the expressions of COX-2 mRNA were decreased significantly in the No.2RFR group and the celebrex group, but the expressions of COX-1 mRNA had no differences among the four groups.

No.2RFR can improve renal function and reduce glomerular sclerosis and renal fibrosis by inhibiting the over-expression of the COX-2 mRNA.

Key words: Chronic renal failure, Cycloxygenase-2, Glomerular sclerosis, Renal fibrosis, Rats


Group SCr (μmol/L) BUN (mmol/L)
n Before treatment n After treatment n Before treatment n After treatment
Normal control 10 37.80±4.42 9 42.00±3.22 10 4.50±0.33 9 4.87±0.43
Untreated 10 123.91±24.10** 7 117.00±10.39** 10 11.78±0.91** 7 10.46±0.43**
Celebrex 10 105.10±87.67** 8 65.86±7.67**△ 10 8.40±0.94** 8 7.38±0.42**△
No.2RFR 10 118.56±21.33** 9 54.56±8.63**△△▲▲ 10 11.38±1.10** 9 7.13±0.67**△△

Figure 1

Nephridial tissue characteristics of rats in different groups observed by light microscopy (HE staining, ×400) A: Normal control group; B: Untreated group; C: No.2RFR group; D: Celebrex group."


Normal control 9 0.198 7±0.110 6 0.218 7±0.246 4
Untreated 7 2.690 7±0.341 7** 0.334 8±0.074 5
Celebrex 8 1.100 5±0.445 4**△△ 0.529 2±0.415 6
No.2RFR 9 0.553 5±0.589 9**△△▲▲ 0.458 7±0.362 6

Figure 2

Expressions of COX-1 and COX-2 mRNAs in renal cortex of rats in different groups 1: Marker; 2: Normal control group; 3: Untreated group; 4: No.2RFR group; 5: Celebrex group. Each group includes three strips."

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