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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (5): 546-552.doi: 10.3736/jcim20110513

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Huaiqihuang Granule on nephrin and podocin expressions in renal tissues of rats with adriamycin-induced nephrosis

Wen Sun1, Zhi Zhu1, Jian Yu1(), Yong-hong Wang1, Man Xiong2, Xia Gao3, Zhong-hua Zhao4, Xue-guang Liu4   

  1. 1. Department of Traditional Chinese Medicine, Children's Hospital, Fudan University, Shanghai 201102, China
    2. Pediatric Institute, Children's Hospital, Fudan University, Shanghai 201102, China
    3. Department of Nephrology, Children's Hospital, Fudan University, Shanghai 201102, China
    4. Department of Pathology, Shanghai Medical College of Fudan University, Shanghai 200032, China
  • Received:2010-12-12 Accepted:2011-01-09 Online:2011-05-20 Published:2011-05-15
  • Contact: Yu Jian E-mail:yuj@shmu.edu.cn

Object: To investigate the effects of Huaiqihuang Granule, a compound Chinese herbal medicine, on expressions of nephrin and podocin of slit diaphragm of glomerular podocytes in rats with adriamycin-induced nephrosis and to explore the mechanism in reducing the proteinuria.
Methods: Twenty SD rats were randomly divided into five groups: control group, model group, glucocorticoid group, Huaiqihuang Granule group and Huaiqihuang Granule plus glucocorticoid group. The 24-hour urine was collected 7, 14, 21 and 28 days after adriamycin injection respectively to measure 24-hour urinary protein, and all rats were sacrificed after 28-day treatment. Pathological changes in renal tissues were observed under a light microscope and an electron microscope. Expressions of nephrin and podocin mRNAs in renal cortex were determined by real-time polymerase chain reaction, and protein levels of nephrin and podocin were detected by Western blotting.
Results: (1) In the model group and the treatment groups, the level of urinary protein increased significantly from the 14th day. (2) Under the light microscope, inflammatory cells and slight fibroplasia were found in renal interstitium of the model group, but there were less inflammatory cells in renal interstitium in the intervention groups than in the model group. Under the electron microscope, 29 days after adriamycin injection, extensive fusion of foot processes was observed. (3) The expressions of nephrin and podocin were higher in treatment groups than in the model group. (4) Proteinuria level was negatively correlated with the expressions of nephrin mRNA and nephrin and podocin proteins.
Conclusion: The above results indicate that Huaiqihuang Granule can maintain the integrity of the slid diaphragram in podocyte, alleviate the lesion of glomerular filtration membrane, and decrease the proteinuria by up-regulating the expressions of nephrin and podocin. Huaiqihuang Granule plus glucocorticoid maybe has better effects than glucocorticoid alone.

Key words: drugs,Chinese herbal, nephrosis, podocytes, nephrin, podocin, rats

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Figure 1

Pathological changes of renal tissues of rats under the light microscope (Hematoxylin and eosin staining, ×400) A: Control group; B: Model group; C: Huaiqihuang Granule group; D: Glucocorticoid group; E: Huaiqihuang Granule plus glucocorticoid group."

Figure 2

Ultra-structural changes of foot processes of renal tissues of rats under the electron microscope (×7 000) A: Control group; B: Model group; C: Huaiqihuang Granule group; D: Glucocorticoid group; E: Huaiqihuang Granule plus glucocorticoid group."

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Figure 3

Expressions of nephrin and podocin proteins observed by Western blot method A: Control group; B: Model group; C: Huaiqihuang Granule group; D: Glucocorticoid group; E: Huaiqihuang Granule plus glucocorticoid group."

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