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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (5): 546-552.doi: 10.3736/jcim20110513

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Huaiqihuang Granule on nephrin and podocin expressions in renal tissues of rats with adriamycin-induced nephrosis

Wen Sun1, Zhi Zhu1, Jian Yu1(), Yong-hong Wang1, Man Xiong2, Xia Gao3, Zhong-hua Zhao4, Xue-guang Liu4   

  1. 1. Department of Traditional Chinese Medicine, Children's Hospital, Fudan University, Shanghai 201102, China
    2. Pediatric Institute, Children's Hospital, Fudan University, Shanghai 201102, China
    3. Department of Nephrology, Children's Hospital, Fudan University, Shanghai 201102, China
    4. Department of Pathology, Shanghai Medical College of Fudan University, Shanghai 200032, China
  • Received:2010-12-12 Accepted:2011-01-09 Online:2011-05-20 Published:2011-05-15
  • Contact: Yu Jian

Object: To investigate the effects of Huaiqihuang Granule, a compound Chinese herbal medicine, on expressions of nephrin and podocin of slit diaphragm of glomerular podocytes in rats with adriamycin-induced nephrosis and to explore the mechanism in reducing the proteinuria.
Methods: Twenty SD rats were randomly divided into five groups: control group, model group, glucocorticoid group, Huaiqihuang Granule group and Huaiqihuang Granule plus glucocorticoid group. The 24-hour urine was collected 7, 14, 21 and 28 days after adriamycin injection respectively to measure 24-hour urinary protein, and all rats were sacrificed after 28-day treatment. Pathological changes in renal tissues were observed under a light microscope and an electron microscope. Expressions of nephrin and podocin mRNAs in renal cortex were determined by real-time polymerase chain reaction, and protein levels of nephrin and podocin were detected by Western blotting.
Results: (1) In the model group and the treatment groups, the level of urinary protein increased significantly from the 14th day. (2) Under the light microscope, inflammatory cells and slight fibroplasia were found in renal interstitium of the model group, but there were less inflammatory cells in renal interstitium in the intervention groups than in the model group. Under the electron microscope, 29 days after adriamycin injection, extensive fusion of foot processes was observed. (3) The expressions of nephrin and podocin were higher in treatment groups than in the model group. (4) Proteinuria level was negatively correlated with the expressions of nephrin mRNA and nephrin and podocin proteins.
Conclusion: The above results indicate that Huaiqihuang Granule can maintain the integrity of the slid diaphragram in podocyte, alleviate the lesion of glomerular filtration membrane, and decrease the proteinuria by up-regulating the expressions of nephrin and podocin. Huaiqihuang Granule plus glucocorticoid maybe has better effects than glucocorticoid alone.

Key words: drugs,Chinese herbal, nephrosis, podocytes, nephrin, podocin, rats



Figure 1

Pathological changes of renal tissues of rats under the light microscope (Hematoxylin and eosin staining, ×400) A: Control group; B: Model group; C: Huaiqihuang Granule group; D: Glucocorticoid group; E: Huaiqihuang Granule plus glucocorticoid group."

Figure 2

Ultra-structural changes of foot processes of renal tissues of rats under the electron microscope (×7 000) A: Control group; B: Model group; C: Huaiqihuang Granule group; D: Glucocorticoid group; E: Huaiqihuang Granule plus glucocorticoid group."



Figure 3

Expressions of nephrin and podocin proteins observed by Western blot method A: Control group; B: Model group; C: Huaiqihuang Granule group; D: Glucocorticoid group; E: Huaiqihuang Granule plus glucocorticoid group."

[1] Liu XH, Yi ZW, Huang DL, Chen D, Guan FJ, Cao Y . Systematic analysis of therapy of the primary nephrotic syndrome with the traditional Chinese and Western medicine combination[J]. Yi Xue Lin Chuang Yan Jiu, 2007,24(4):549-554
doi: 10.3969/j.issn.1671-7171.2007.04.008
刘喜红, 易著文, 黄丹琳, 陈丹, 关凤军, 曹艳 . 中西医结合治疗原发性肾病综合征的系统评价[J]. 医学临床研究, 2007,24(4):549-554
doi: 10.3969/j.issn.1671-7171.2007.04.008
[2] Editorial Committee of Chinese Herbal Medicine, State Administration of Traditional Chinese Medicine. Chinese herbal medicine(Vol 1)[M]. Shanghai: Shanghai Scientific and Technical Publisher, 1999: 564-565
国家中医药管理局《中华本草》编委会. 中华本草(第一卷)[M]. 上海: 上海科学技术出版社, 1999: 564-565
[3] Chen SB, Ding RN . The effect of Trametes robiniophila Murr.(TRM) substantial composition on immune function of mice[J]. Shi Yong Jun Xue Bao, 1995,2(1):21-25
陈慎宝, 丁如宁 . 槐耳菌质成分对小鼠免疫功能的影响[J]. 食用菌学报, 1995,2(1):21-25
[4] He B, Cai MM, Wu YB . Clinical research on effects of Huai’er granule on quality of life and immune function of patients receiving chemotherapy[J]. Liaoning Zhong Yi Za Zhi, 2007,34(7):943-944
何斌, 蔡明明, 吴燕波 . 槐耳颗粒对化疗患者生活质量及免疫功能影响的临床研究[J]. 辽宁中医杂志, 2007,34(7):943-944
[5] Wang XW, Yang LJ, Qiu FB, Wu LQ, Lu Y . The impact of Huai’er granule to Th1/Th2 shift on patients with primary hepatic cancer after surgical resection[J]. Zhongguo Xian Dai Yao Wu Ying Yong, 2008,2(9):7-8
doi: 10.3969/j.issn.1673-9523.2008.09.004
王晓伟, 杨丽君, 邱法波, 吴力群, 卢云 . 槐耳颗粒对原发性肝癌术后Th1/Th2漂移的影响[J]. 中国现代药物应用, 2008,2(9):7-8
doi: 10.3969/j.issn.1673-9523.2008.09.004
[6] Lowik MM, Groenen PJ, Levtchenko EN, Monnens LA, van den Heuvel LP .Molecular genetic analysis of podocyte genes in focal segmental glomerulosclerosis — a review[J]. Eur J Pediatr, 2009,168(11):1291-1304
doi: 10.1007/s00431-009-1017-x pmid: 19562370
[7] Ruotsalainen V, Ljungberg P, Wartiovaara J, Lenkkeri U, Kestila M, Jalanko H, Holmberg C, Tryggvason K .Nephrin is specifically located at the slit diaphragm of glomerular podocytes[J]. Proc Natl Acad Sci U S A, 1999,96(14):7962-7967
doi: 10.1073/pnas.96.14.7962
[8] Otaki Y, Miyauchi N, Higa M, Takada A, Kuroda T, Gejyo F, Shimizu F, Kawachi H . Dissociation of NEPH1 from nephrin is involved in development of a rat model of focal segmental glomerulosclerosis[J]. Am J Physiol Renal Physiol, 2008,295(5):F1376-F1387
doi: 10.1152/ajprenal.00075.2008
[9] Cooper ME, Mundel P, Boner G . Role of nephrin in renal disease including diabetic nephropathy[J]. Semin Nephrol, 2002,22(5):393-398
doi: 10.1053/snep.2002.34724
[10] Okuda S, Oh Y, Tsuruda H, Onoyama K, Fujimi S, Fujishima M . Adriamycin-induced nephropathy as a model of chronic progressive glomerular disease[J]. Kidney Int, 1986,29(2):502-510
doi: 10.1038/ki.1986.28 pmid: 3486312
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