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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (5): 553-557.doi: 10.3736/jcim20110514

• Original Experimental Research • Previous Articles     Next Articles

Effects of Herba Ephedra Sinicae and Fructus Schisandrae Chinensis on pathology of rats with bleomycin A5-induced idiopathic pulmonary fibrosis

Hua-qiang Zhai1,2,3, Shuo-feng Zhang2, Ming-chao Gao2, Yang Liu2, Min Ou4, Fan-yun Meng1,3(), Yong-yan Wang1,3   

  1. 1. Beijing Key Laboratory of Protection and Utilization of Chinese Medicine, Beijing Normal University, Beijing 100875, China
    2. School of Chinese Materia Metica, Beijing University of Chinese Medicine, Beijing 100102, China
    3. Institute of Natural Medicine and Chinese Medicine Resources, Beijing Normal University, Beijing 100875, China
    4. Department of Respiratory Diseases, Naval General Hospital, Beijing 100037, China
  • Received:2010-12-27 Accepted:2011-02-24 Online:2011-05-20 Published:2011-05-15
  • Contact: Meng Fan-yun

Objective: To observe the different effects between Mahuang (Herba Ephedra Sinicae) and Wuweizi (Fructus Schisandrae Chinensis) on the pathological changes of rats with bleomycin A5-induced pulmonary fibrosis.
Methods: Ninety Wistar rats were randomly divided into sham operation group, model group, hydrocortisone group, Herba Ephedra Sinicae group, Fructus Schisandrae Chinensis group and Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group. There were 16 rats in each group except the sham operation group (10 rats). Pulmonary fibrosis was induced by a single intratracheal injection of bleomycin A5. Hematoxylin and eosin straining and immunohistochecical method were used after 7- and 28-day treatment to observe the pathology of lung injury, measure the inner diameter of pulmonary arterioles and the density of nuclear membrane.
Results: Compared with the sham operation group at 7 and 28 d, alveolar inflammation level was significantly increased in the model group (P<0.01). Alveolar inflammation level was decreased obviously in the hydrocortisone group (P<0.05) after 7- and 28-day treatment as compared with the model group, and that in Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group was also decreased obviously (P<0.05) at 28 d. Compared with the sham operation group, nuclear density of the model group was increased, while its inner diameter was decreased (P<0.05). In the Fructus Schisandrae Chinensis group, Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group and hydrocortisone group, the nuclear density was decreased (P<0.05) as compared with the model group. Inner diameter in the Herba Ephedra Sinicae group, Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group and hydrocortisone group was higher than that in the model group (P<0.05). Microvessel density of the model group was obviously higher than that of the sham operation group (P<0.05). Compared with the model group, Herba Ephedra Sinicae plus Fructus Schisandrae Chinensis group and hydrocortisone group had lower microvessel density (P<0.05).
Conclusion: Herba Ephedra Sinicae combined with Fructus Schisandrae Chinensis can restrain pulmonary artery injury. The nuclear density and microvessel density can be reduced by Fructus Schisandrae Chinensis, while Herba Ephedra Sinicae can increase the inner diameter.

Key words: pulmonary fibrosis, pathology, Herba Ephedra Sinicae, Fructus Schisandrae Chinensis , rats

Figure 1

Hematoxylin-eosin staining of pulmonary tissues in different groups 28 d after operation (Light microscopy, ×400) A: Sham operation group; B: Model group; C: Herba Ephedra Sinicae group; D: Fructus Schisandrae Chinensis group."



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