SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (6): 643-650.doi: 10.3736/jcim20110611

• Original Experimental Research • Previous Articles     Next Articles

Changes of protein expression profile in vascular tissues of spontaneously hypertensive rats treated by a compound Chinese herbal medicine

Rong Fan1, Feng He2, Yang Wang1, Guang-wei Zhong1(), Yun-hui Li1   

  1. 1. Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
    2. Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
  • Received:2011-02-27 Accepted:2011-03-08 Online:2011-06-20 Published:2011-06-15
  • Contact: Zhong Guang-wei E-mail:zgw7512@sina.com

RichHTML

1

PDF (PC)

54

Like

Objective: To investigate the effects of a Chinese herbal formula for calming liver and suppressing yang on the protein expressions of vascular tissues in spontaneously hypertensive rats (SHRs), and to explore the mechanism of efficacy.
Methods: Twenty SHRs were randomly divided into model group and treatment group. Another 10 Wistar-Kyoto rats were selected as a normal control. SHRs in the treatment group were administered with the formula for calming liver and suppressing Yang for 4 weeks. During the course of treatment, blood pressure and heart rates were monitored every week and the ethology of rats, including irritability and rotation endurance was also evaluated. After treatment, thoracic aorta was obtained and its proteins were separated by 2-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and database query.
Results: The formula for calming liver and suppressing yang not only decreased the systolic blood pressure and heart rate, but also improved irritability degree and rotation endurance time of SHRs. This experiment had established the 2-DE graph of protein expressions of vascular tissues in SHRs. Compared with the normal group, the expressions of 15 proteins were down-regulated, and 12 proteins were up-regulated in vascular tissues of the model group. The formula for calming liver and suppressing yang treatment up-regulated expressions of 10 proteins in the 15 down-regulated proteins, and down-regulated 8 proteins in the 12 up-regulated proteins in vascular tissues of SHRs. After analysis, 16 obviously differentially expressed proteins were found, and 13 of them were identified.
Conclusion: The formula for calming liver and suppressing yang can improve the ethology of SHRs. The mechanism is probably concerned with regulating the protein expressions of vascular tissues.

Key words: drugs,Chinese herbal, hypertensions, proteome, vascular tissue, rats

"

"

"

Figure 1

Two-dimensional electrophoresis map of proteins from vascular tissue of rats in three groups C: Normal group; M: Model group; Z: Treatment group. SDS-PAGE: sodium dodecylsulfate-polyacrylamide gel electrophoresis; PI: isoelectric point."

Figure 2

Peptide mass fingerprinting of mitofusin-2 by MALDI-TOF MS analysis MALDI-TOF MS: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry."

Figure 3

Mascot search results of peptide mass fingerprinting of mitofusin-2 in Swissport"

"

[1] Messerli FH, Williams B, Ritz E . Essential hypertension[J]. Lancet, 2007,370(9587):591-603
doi: 10.1016/S0140-6736(07)61299-9
[2] Zhong GW, Luo YH, Xiang LL, Xie Y, Xie QY, Li YH, Zhang C . Clinical efficacy study on calming liver and restraining yang formula in treating patients with mild or moderate degree of essential hypertension[J]. Zhongguo Zhong Yao Za Zhi, 2010,35(6):776-781
doi: 10.4268/cjcmm20100626
钟广伟, 罗艳红, 相玲丽, 谢勇, 谢启应, 李云辉, 张臣 . 平肝潜阳方治疗早、中期原发性高血压病患者的临床疗效研究[J]. 中国中药杂志, 2010,35(6):776-781
doi: 10.4268/cjcmm20100626
[3] Hu JJ, Chen ZQ, Zhong GW, Li W, Yin YH . Comparative proteomics analysis on splenic lymphocytes in migraine rat model with hyperactivity of liver-yang[J]. Zhongguo Zhong Yi Yao Xin Xi Za Zhi, 2008,15(8):34-37
doi: 10.3969/j.issn.1005-5304.2008.08.016
胡建军, 陈泽奇, 钟广伟, 李炜, 尹耀慧 . 偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学分析[J]. 中国中医药信息杂志, 2008,15(8):34-37
doi: 10.3969/j.issn.1005-5304.2008.08.016
[4] Yang LH, Zou SZ, Li YX . A new apparatus and method in no-trauma measuring of systolic pressure and diastolic pressure of rats[J]. Zhongguo Ying Yong Sheng Li Xue Za Zhi, 1991,7(1):62-64
杨绿化, 邹沙舟, 李云霞 . 无创测量大鼠收缩压和舒张压的新仪器和新方法[J]. 中国应用生理学杂志, 1991,7(1):62-64
[5] Yan DH, Jin YQ, Xiao C, Hu SY, Wang YH, Xiao GL . A model with liver-yang hyperactivity in spontaneously hypertensive rats[J]. Hunan Zhong Yi Xue Yuan Xue Bao, 1999,19(4):35-38
鄢东红, 金益强, 肖纯, 胡随瑜, 王勇华, 肖桂林 . 自发性高血压大鼠肝阳上亢证模型的复制[J]. 湖南中医学院学报, 1999,19(4):35-38
[6] Li MY, Xiao ZQ, Li C, Wu XY, Feng XP, Yi H, Li JL, Chen ZC, Chen P, Liang SP . Establishment of protein profile of human small cell lung cancer cell line NCI-H446[J]. Ai Zheng, 2004,23(10):1116-1121
doi: 10.3321/j.issn:1000-467X.2004.10.003
李茂玉, 肖志强, 李萃, 吴晓英, 冯雪萍, 易红, 李建玲, 陈主初, 陈平, 梁宋平 . 小细胞肺癌细胞系NCI-H446蛋白质表达谱的建立[J]. 癌症, 2004,23(10):1116-1121
doi: 10.3321/j.issn:1000-467X.2004.10.003
[7] Liu ZR . Recent developments of epidemiologic survey about hypertension in China[J]. Yi Xue Zong Shu, 2004,10(2):88-89
刘忠仁 . 我国各地高血压流行病学调查近况[J]. 医学综述, 2004,10(2):88-89
[8] Ferguson SA, Paule MG, Cada A, Fogle CM, Gray EP, Berry KJ . Baseline behavior, but not sensitivity to stimulant drugs, differs among spontaneously hypertensive, Wistar-Kyoto, and Sprague-Dawley rat strains[J]. Neurotoxicol Teratol, 2007,29(5):547-561
doi: 10.1016/j.ntt.2007.07.001
[9] Cai GX, Zhu KJ, Han YM, Sun HL . Clinical epidemiology of common syndromes of hypertension[J]. Zhong Yi Za Zhi, 1999,40(8):492-493
蔡光先, 朱克俭, 韩育明, 孙慧兰 . 高血压病常见证候临床流行病学观察[J]. 中医杂志, 1999,40(8):492-493
[10] Chen KJ, Ma XC, Zhang JC . The positive meaning in the new hypertension guidelines issued by the Joint National Committee(JNC-7)[J]. Zhong Xi Yi Jie He Xin Nao Xue Guan Bing Za Zhi, 2003,1(6):311
doi: 10.3969/j.issn.1672-1349.2003.06.001
陈可冀, 马晓昌, 张京春 . 关于美国新高血压指南(JNC-7)的积极意义[J]. 中西医结合心脑血管病杂志, 2003,1(6):311
doi: 10.3969/j.issn.1672-1349.2003.06.001
[11] Terrier B, Tamby MC, Camoin L, Guilpain P, Broussard C, Bussone G , Yaci A, Hotellier F, Simonneau G, Guillevin L, Humbert M, Mouthon L.Identification of target antigens of antifibroblast antibodies in pulmonary arterial hypertension[J]. Am J Respir Cfit Care Med, 2008,177(10):1128-1134
doi: 10.1164/rccm.200707-1015OC
[12] Zhong GW, Li W, Luo YH, Chen MJ, Yi ZJ, Chen GL, Xiang LL . Effects of the calming liver and suppressing yang method on proliferation and the expression of heat shock protein 27 in vascular smooth muscle cells of spontaneously hypertensive rats[J]. Zhongguo Lao Nian Xue Za Zhi, 2009,29(4):385-388
doi: 10.3969/j.issn.1005-9202.2009.04.001
钟广伟, 李炜, 罗艳红, 陈民敬, 易振佳, 陈国林, 相玲丽 . 平肝潜阳法对SHR血管平滑肌细胞增殖及HSP27表达的影响[J]. 中国老年学杂志, 2009,29(4):385-388
doi: 10.3969/j.issn.1005-9202.2009.04.001
[13] Touyz RM, He Y, Montezano AC, Yao G, Chubanov V, Gudermann T, Callera GE . Differential regulation of transient receptor potential melastatin 6 and 7 cation channels by ANG Ⅱ in vascular smooth muscle cells from spontaneously hypertensive rats[J]. Am J Physiol Regul Integr Comp Physiol, 2006,290(1):R73-R78
doi: 10.1152/ajpregu.00515.2005
[14] Alldridge LC, Bryant CE . Annexin 1 regulates cell proliferation by disruption of cell morphology and inhibition of cyclin D1 expression through sustained activation of the ERK1/2 MAPK signal[J]. Exp Cell Res, 2003,290(1):93-107
doi: 10.1016/S0014-4827(03)00310-0
[15] Neuspiel M, Zunino R, Gangaraju S, Rippstein P, McBride H .Activated mitofusin 2 signals mitochondrial fusion, interferes with Bax activation, and reduces susceptibility to radical induced depolarization[J]. J Biol Chem, 2005,280(26):25060-25070
doi: 10.1074/jbc.M501599200
[16] Chen KH, Guo X, Ma D, Guo Y, Li Q, Yang D, Li P, Qiu X, Wen S, Xiao RP, Tang J . Dysregulation of HSG triggers vascular proliferative disorders[J]. Nat Cell Biol, 2004,6(9):872-883
doi: 10.1038/ncb1161 pmid: 15322553
[17] Guo X, Chen KH, Guo Y, Liao H, Tang J, Xiao RP . Mitofusin 2 triggers vascular smooth muscle cell apoptosis via mitochondrial death pathway[J]. Circ Res, 2007,101(11):1113-1122
doi: 10.1161/CIRCRESAHA.107.157644 pmid: 17901359
[18] Sahai E, Marshall CJ . RHO-GTPases and cancer[J]. Nat Rev Cancer, 2002,2(2):133-142
doi: 10.1038/nrc725 pmid: 12635176
[19] Bi HM, Li D, Dan HQ . Effects of hypertension on complications of capillary vessels and nerves in patients with type 2 diabetes mellitus[J]. Zhongguo Zong He Lin Chuang, 2000,16(12):899-900
doi: 10.3760/cma.j.issn.1008-6315.2000.12.012
毕会民, 李栋, 但汉卿 . 高血压对Ⅱ型糖尿病微血管和神经并发症的影响[J]. 中国综合临床, 2000,16(12):899-900
doi: 10.3760/cma.j.issn.1008-6315.2000.12.012
[1] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[8] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[9] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[12] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[13] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[14] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[15] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3