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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (9): 983-990.doi: 10.3736/jcim20110909

• Original Experimental Research • Previous Articles     Next Articles

Pharmacological effects of Oleum Cinnamomi and water extract of Cortex Cinnamomi in rats with yang-deficiency cold syndrome and the mathematical analysis

Qian Zhang, Bing Zhang(), Rui Jin, Lian-zhen Li, Qian Zhao, Xiao-qing Liu   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2011-03-08 Accepted:2011-04-21 Online:2011-09-20 Published:2011-09-15
  • Contact: Zhang Bing

Objective:To compare the effects of Oleum Cinnamomi and water extract of Cortex Cinnamomi in rats with yang-deficiency cold syndrome based on mathematical analysis.

Methods:A total of 48 male Sprague-Dawley rats were randomly divided into 4 groups (normal, model, Oleum Cinnamomi and water extract of Cortex Cinnamomi). Yang-deficiency cold syndrome was induced by hydrocortisone sodium succinate. Oleum Cinnamomi and water extract of Cortex Cinnamomi were given by gastrogavage daily for one week to respective groups. Material metabolism indexes such as glucose (GLU), uric acid (UA), total cholesterol (TC), triacylylyceral (TAG), total protein (TP) and albumin (ALB); energy metabolism indexes such as lactic acid (LAC), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and adenosine triphosphatase (ATPase); endocrine system indexes such as corticosteroid (CS), triiodothyronine (T3), tetraiodothyronine (T4), thyroid stimulating hormone (TSH), estradiol (E2), vitamin C (VC) and 17-hydroxycorticosteriod (17-OHCS); and immune system indexes such as immunoglobulin M (IgM), immunoglobulin G (IgG), complement 3 (C3) and complement 4 (C4) were measured. And then the data were analyzed by mathematical analysis method.

Results: Oleum Cinnamomi and water extract of Cortex Cinnamomi had similar influence on some indexes of material metabolism, energy metabolism and endocrine and immune systems in rats with yang-deficiency cold syndrome. Positive effects were showed in GLU, TC, TAG, TP, ALB, IgM, E2, CS, VC and 17-OHCS. Water extract of Cortex Cinnamomi had no significant effects on TSH, LAC and SDH, but Oleum Cinnamomi had, while water extract of Cortex Cinnamomi had significant effects on ATPase, LDH and IgG.

Conclusion: There is a complex relationship between Oleum Cinnamomi and water extract of Cortex Cinnamomi in their effects on rats with yang-deficiency cold syndrome. They have similar property, however, disparities exist between them to some extent. The exact mechanism needs further research.

Key words: Cinnamomum cassia, drugs, Chinese herbal, plant extracts, yang-deficiency cold, properties and flavous,traditional Chinese drugs, mathematical computing, rats

Table 1

Comparison of changes of material metabolism indexes in rats of different groups ($\overline{x}$±s)"

Group n GLU (mmol/L) UA (mmol/L) TC (mmol/L) TAG (mmol/L) TP (g/L) ALB (g/L)
Normal 12 6.12±1.14 91.04±8.49 1.34±0.22 1.15±0.52 81.05±2.94 32.85±1.17
Model 12 5.17±1.38 122.58±24.30** 1.13±0.17** 0.75±0.17* 89.30±6.08** 34.43±1.65*
Oleum Cinnamomi (400 mg/kg) 12 6.70±1.22△△ 125.30±10.83 1.35±0.26 0.93±0.17 87.28±3.99 33.76±1.30
Water extract (30 g/kg) 12 5.90±1.79 127.15±18.13 1.34±0.19△△ 0.98±0.27 86.91±4.51 33.60±1.19

Table 2

Comparison of changes of ATPase in rats of different groups ($\overline{x}$±s, μmol pi/(mg prot·h))"

Group N Na+-K+-ATPase Mg2+-ATPase Ca2+-ATPase Ca2+-Mg2+-ATPase
Normal 12 3.95±0.76 4.14±0.76 3.89±0.91 4.12±0.87
Model 12 3.41±0.25* 3.01±0.27** 3.12±0.22* 3.38±0.29**
Oleum Cinnamomi (400 mg/kg) 12 3.07±0.47 2.66±0.27 3.04±0.45 3.36±0.17
Water extract (30 g/kg) 12 3.61±0.40 3.16±0.41 3.56±0.55 3.54±0.39

Table 3

Comparison of changes of LAC, LDH and SDH in rats of different groups ($\overline{x}$±s)"

Group n LAC (U/L) LDH (mmol/L) SDH (U/mg protein)
Normal 12 69.98±12.21 1 973.80±697.02 5.02±1.21
Model 12 59.33±9.77* 1 284.50±326.51** 3.87±0.52*
Oleum Cinnamomi (400 mg/kg) 12 49.59±1.87 1 354.00±417.67 4.43±1.55
Water extract (30 g/kg) 12 59.59±4.83 1 903.50±313.55△△ 3.99±1.12

Table 4

Comparison of changes of T3, T4, TSH, E2 and CS in rats of different groups($\overline{x}$±s)"

Group n T3 (μg/L) T4 (μg/L) TSH (IU/L) E2 (ng/L) CS (μg/L)
Normal 12 0.40±0.07 56.90±5.00 390±90 77.74±14.79 23.63±3.08
Model 12 0.32±0.06* 48.31±6.41** 560±190* 101.84±18.78** 21.13±2.48
Oleum Cinnamomi (400 mg/kg) 12 0.25±0.04△△ 44.39±5.48 390±140 81.91±11.83 22.02±2.98
Water extract (30 g/kg) 12 0.38±0.05 53.46±6.61 520±110 84.82±14.95 23.16±2.00

Table 5

Comparison of changes of VC and 17-OHCS in rats of different groups ($\overline{x}$±s)"

Group n OD value
Normal 12 0.44±0.04 0.24±0.02
Model 12 0.51±0.09* 0.21±0.02**
Oleum Cinnamomi
(400 mg/kg)
12 0.43±0.03 0.23±0.03
Water extract (30 g/kg) 12 0.45±0.04 0.23±0.01

Table 6

Comparison of changes of IgM, IgG, C3 and C4 in rats of different groups ($\overline{x}$±s, mg/L)"

Group n IgM IgG C3 C4
Normal 12 418.6±24.8 792.9±120.1 592.3±52.0 59.3±6.7
Model 12 401.3±49.3 670.7±95.3* 758.0±66.1** 85.4±14.5**
Oleum Cinnamomi (400 mg/kg) 12 430.8±67.9 655.2±73.4 822.5±79.6△△ 87.0±11.9
Water extract (30 g/kg) 12 437.3±58.7 752.7±125.5 821.3±91.0△△ 82.4±11.7

Figure 1

Results of mathematical analysis"

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