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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (9): 983-990.doi: 10.3736/jcim20110909

• Original Experimental Research • Previous Articles     Next Articles

Pharmacological effects of Oleum Cinnamomi and water extract of Cortex Cinnamomi in rats with yang-deficiency cold syndrome and the mathematical analysis

Qian Zhang, Bing Zhang(), Rui Jin, Lian-zhen Li, Qian Zhao, Xiao-qing Liu   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2011-03-08 Accepted:2011-04-21 Online:2011-09-20 Published:2011-09-15
  • Contact: Zhang Bing E-mail:zhangbing6@263.net

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Objective:To compare the effects of Oleum Cinnamomi and water extract of Cortex Cinnamomi in rats with yang-deficiency cold syndrome based on mathematical analysis.

Methods:A total of 48 male Sprague-Dawley rats were randomly divided into 4 groups (normal, model, Oleum Cinnamomi and water extract of Cortex Cinnamomi). Yang-deficiency cold syndrome was induced by hydrocortisone sodium succinate. Oleum Cinnamomi and water extract of Cortex Cinnamomi were given by gastrogavage daily for one week to respective groups. Material metabolism indexes such as glucose (GLU), uric acid (UA), total cholesterol (TC), triacylylyceral (TAG), total protein (TP) and albumin (ALB); energy metabolism indexes such as lactic acid (LAC), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and adenosine triphosphatase (ATPase); endocrine system indexes such as corticosteroid (CS), triiodothyronine (T3), tetraiodothyronine (T4), thyroid stimulating hormone (TSH), estradiol (E2), vitamin C (VC) and 17-hydroxycorticosteriod (17-OHCS); and immune system indexes such as immunoglobulin M (IgM), immunoglobulin G (IgG), complement 3 (C3) and complement 4 (C4) were measured. And then the data were analyzed by mathematical analysis method.

Results: Oleum Cinnamomi and water extract of Cortex Cinnamomi had similar influence on some indexes of material metabolism, energy metabolism and endocrine and immune systems in rats with yang-deficiency cold syndrome. Positive effects were showed in GLU, TC, TAG, TP, ALB, IgM, E2, CS, VC and 17-OHCS. Water extract of Cortex Cinnamomi had no significant effects on TSH, LAC and SDH, but Oleum Cinnamomi had, while water extract of Cortex Cinnamomi had significant effects on ATPase, LDH and IgG.

Conclusion: There is a complex relationship between Oleum Cinnamomi and water extract of Cortex Cinnamomi in their effects on rats with yang-deficiency cold syndrome. They have similar property, however, disparities exist between them to some extent. The exact mechanism needs further research.

Key words: Cinnamomum cassia, drugs, Chinese herbal, plant extracts, yang-deficiency cold, properties and flavous,traditional Chinese drugs, mathematical computing, rats

Table 1

Comparison of changes of material metabolism indexes in rats of different groups （$\overline{x}$±s）"

Group n GLU (mmol/L) UA (mmol/L) TC (mmol/L) TAG (mmol/L) TP (g/L) ALB (g/L)
Normal 12 6.12±1.14 91.04±8.49 1.34±0.22 1.15±0.52 81.05±2.94 32.85±1.17
Model 12 5.17±1.38 122.58±24.30** 1.13±0.17** 0.75±0.17* 89.30±6.08** 34.43±1.65*
Oleum Cinnamomi (400 mg/kg) 12 6.70±1.22△△ 125.30±10.83 1.35±0.26△ 0.93±0.17△ 87.28±3.99 33.76±1.30
Water extract (30 g/kg) 12 5.90±1.79 127.15±18.13 1.34±0.19△△ 0.98±0.27△ 86.91±4.51 33.60±1.19

Table 2

Comparison of changes of ATPase in rats of different groups （$\overline{x}$±s, μmol pi/(mg prot·h)）"

Group N Na+-K+-ATPase Mg2+-ATPase Ca2+-ATPase Ca2+-Mg2+-ATPase
Normal 12 3.95±0.76 4.14±0.76 3.89±0.91 4.12±0.87
Model 12 3.41±0.25* 3.01±0.27** 3.12±0.22* 3.38±0.29**
Oleum Cinnamomi (400 mg/kg) 12 3.07±0.47 2.66±0.27△ 3.04±0.45 3.36±0.17
Water extract (30 g/kg) 12 3.61±0.40 3.16±0.41 3.56±0.55△ 3.54±0.39

Table 3

Comparison of changes of LAC, LDH and SDH in rats of different groups （$\overline{x}$±s）"

Group n LAC （U/L） LDH （mmol/L） SDH (U/mg protein)
Normal 12 69.98±12.21 1 973.80±697.02 5.02±1.21
Model 12 59.33±9.77* 1 284.50±326.51** 3.87±0.52*
Oleum Cinnamomi (400 mg/kg) 12 49.59±1.87△ 1 354.00±417.67 4.43±1.55△
Water extract (30 g/kg) 12 59.59±4.83 1 903.50±313.55△△ 3.99±1.12

Table 4

Comparison of changes of T3, T4, TSH, E2 and CS in rats of different groups（$\overline{x}$±s）"

Group n T3 （μg/L） T4 （μg/L） TSH （IU/L） E2 （ng/L） CS （μg/L）
Normal 12 0.40±0.07 56.90±5.00 390±90 77.74±14.79 23.63±3.08
Model 12 0.32±0.06* 48.31±6.41** 560±190* 101.84±18.78** 21.13±2.48
Oleum Cinnamomi (400 mg/kg) 12 0.25±0.04△△ 44.39±5.48 390±140△ 81.91±11.83△ 22.02±2.98
Water extract (30 g/kg) 12 0.38±0.05△ 53.46±6.61 520±110 84.82±14.95△ 23.16±2.00

Table 5

Comparison of changes of VC and 17-OHCS in rats of different groups （$\overline{x}$±s）"

Group n OD value
VC 17-OHCS
Normal 12 0.44±0.04 0.24±0.02
Model 12 0.51±0.09* 0.21±0.02**
Oleum Cinnamomi
(400 mg/kg)		 12 0.43±0.03△ 0.23±0.03
Water extract (30 g/kg) 12 0.45±0.04 0.23±0.01

Table 6

Comparison of changes of IgM, IgG, C3 and C4 in rats of different groups （$\overline{x}$±s, mg/L）"

Group n IgM IgG C3 C4
Normal 12 418.6±24.8 792.9±120.1 592.3±52.0 59.3±6.7
Model 12 401.3±49.3 670.7±95.3* 758.0±66.1** 85.4±14.5**
Oleum Cinnamomi (400 mg/kg) 12 430.8±67.9 655.2±73.4 822.5±79.6△△ 87.0±11.9
Water extract (30 g/kg) 12 437.3±58.7 752.7±125.5 821.3±91.0△△ 82.4±11.7

Figure 1

Results of mathematical analysis"

[1] Zhang B, Zhai HQ, Lin ZJ, Ni L . Core structure of Chinese herbal medicine properties based on the “three-factor” theory[J]. Beijing Zhong Yi Yao Da Xue Xue Bao, 2007,30(10):656-657
张冰, 翟华强, 林志健, 倪莉 . 从“三要素”理念探讨中药药性之核心构成[J]. 北京中医药大学学报, 2007,30(10):656-657
[2] Zhu ZP, Zhang MF, Shen YQ, Chen GJ . Pharmacological study on spleen-stomach warming and analgesic action of Cinnamomun cassia Presl[J]. Zhongguo Zhong Yao Za Zhi, 1993,18(9):553-557
朱自平, 张明发, 沈雅琴, 陈光娟 . 肉桂的温中止痛药理研究[J]. 中国中药杂志, 1993,18(9):553-557
[3] Li P, Liu X, Liang DY, Wang F, Sheng X . Effects of Cortex Cinnamomi extract and cinnamic aldehyde on the changes of human body surface microcirculation and local temperature[J]. Zhongguo Zhong Yao Za Zhi, 2006,31(3):262-264
李萍, 刘欣, 梁代英, 王芳, 盛巡 . 肉桂提取物及桂皮醛对人体表微循环及局部温度相关变化的影响[J]. 中国中药杂志, 2006,31(3):262-264
[4] Ding YY, Xie YH, Miao S, Liao B, Wang SW . Effect of Oleum Cinnamomi on mice viral myocarditis caused by CVB3m[J]. Di Si Jun Yi Da Xue Xue Bao, 2005,26(11):1037-1040
doi: 10.3321/j.issn:1000-2790.2005.11.023
丁媛媛, 谢艳华, 缪珊, 廖博, 王四旺 . 肉桂油治疗小鼠CVB3m病毒性心肌炎的实验研究[J]. 第四军医大学学报, 2005,26(11):1037-1040
doi: 10.3321/j.issn:1000-2790.2005.11.023
[5] Chinese Pharmacopoeia Commission. Pharmacopoeia of People’s Republic of China.(Part 2)[M]. Beijing: Chemical Industry Press,2005,(Part 2):Appendix 35
国家药典委员会. 中华人民共和国药典(二部)[M]. 北京: 化学工业出版社,2005,(二部):附录35
[6] Yang Y, Liang YH, Wang CZ, Ren H . Research of time phases property of neuroendocrine immune and hemorheology on deficiency of cold and hot syndrome rats[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2002,8(2):29-32
杨勇, 梁月华, 汪长中, 任红 . 虚寒、虚热证大鼠神经、内分泌、免疫与血液流变学的时相性研究[J]. 中国中医基础医学杂志, 2002,8(2):29-32
[7] Jin R, Lin Q, Liu SM, Zhao Q, Zhang Q, Zhang B . Mathematical analysis of the relationship between yang deficiency syndrome in traditional Chinese medicine and its objective indicators in clinical literature[J]. J Chin Integr Med, 2010,8(7):678-683
金锐, 林茜, 刘森茂, 赵茜, 张倩, 张冰 . 基于数学方法的中医“阳虚证”与临床文献中客观指标的关联分析[J]. 中西医结合学报, 2010,8(7):678-683
[8] Ni L, Zhang B . Re-recognition of animal model of both yin and yang deficiency induced by glucocorticoid[J]. Beijing Zhong Yi Yao Da Xue Xue Bao, 2009,32(5):327-330
倪莉, 张冰 . 糖皮质激素诱导阴虚阳虚状态动物模型的再认识[J]. 北京中医药大学学报, 2009,32(5):327-330
[9] Jin R, Zhang B, Liu SM, Ni L, Li M, Li LZ . Mathematical analysis of characteristics of glucocorticoid-induced yang deficiency or yin deficiency syndrome in animal models based on information entropy theory[J]. J Chin Integr Med, 2011,9(1):15-21
金锐, 张冰, 刘森茂, 倪莉, 李敏, 李连珍 . 基于数据信息熵探讨糖皮质激素诱导的阳虚或阴虚证候动物模型的状态特征[J]. 中西医结合学报, 2011,9(1):15-21
[10] Chang WZ, Liu SM, Lu F . Analysis and prospect of relationship between Chinese herb property and pharmacological effects[J]. Shizhen Guo Yi Guo Yao, 2009,20(3):633-634
doi: 10.3969/j.issn.1008-0805.2009.03.062
常惟智, 刘树民, 卢芳 . 中药药性与功效关联性的研究分析与展望[J]. 时珍国医国药, 2009,20(3):633-634
doi: 10.3969/j.issn.1008-0805.2009.03.062
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