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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (11): 1234-1241.doi: 10.3736/jcim20111112

• Original Experimental Research • Previous Articles     Next Articles

Analysis of major herbs in Chinese herbal formula Jianpi Huoxue Decoction for improving intestinal permeability based on uniform design

Qi-lin Fu1, Yi-yang Hu1,2, Qin Feng1(), Xiao-ning Wang1,2, Jing-hua Peng1, Tuan Cui1   

  1. 1. Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. E-institute of Traditional Chinese Internal Medicine of Shanghai Municipal Education Commission, Shanghai 201203, China
  • Received:2011-07-18 Accepted:2011-07-30 Online:2011-11-20 Published:2011-11-15

Objective: To investigate the herbal medicines which play a main role in Chinese herbal formula Jianpi Huoxue Decoction for improving intestinal permeability and protect alcohol-induced liver injury and intestine damage, and to explore the analysis method for the material base of pharmacological effects of the Chinese herbal compound.
Methods: Sprague Dawley rats were given Lieber-DeCarli ethanol liquid diet once daily for 6 weeks to induce alcoholic liver injury. In step one, U17(1716) table of uniform design was adopted to design the experiment and the eight herbs of Jianpi Huoxue Decoction were screened to seek the herbs which play the main role. Three and a half hours before the rats were killed, each rat was administered lipopolysaccharide once, then blood sample was collected from portal vein and endotoxin content in plasma was detected as the index of intestinal permeability. The data were analyzed by stepwise regression to find the herbal drugs which had the best effects and the compatibility ratio of these drugs. In step two, the rats with Lieber-DeCarli liquid diet-induced liver injury were divided into four groups to test and verify the results.
Results: According to the obtained regression equation, Rasux Paeoniae Alba (Baishao), Rhizoma Alismatis (Zexie) and Fructus Schisandrae Chinensis (Wuweizi) were the main herbal drugs in Jianpi Huoxue Decoction in improving intestinal permeability, and the doses for rats were 1.33, 0.50 and 0.17 g/kg respectively. In the verification experiment, combination of Baishao, Zexie and Wuweizi significantly decreased the endotoxin level in plasma of rats with Lieber-DeCarli-induced liver injury and showed reliability.
Conclusion: Baishao, Zexie and Wuweizi are the major herbs of Jianpi Huoxue Decoction for improving intestinal permeability. Uniform design is efficient in screening the major herbs or their optimal combination in a certain Chinese compound.

Key words: drugs Chinese herbal, Jianpi Huoxue Decoction, liver diseases, alcoholic, uniform design, dose-response relationship, drug, rats

Table 1

Experimentation protocol of uniform design (g/kg)"

Herbal dosage

Group

Atractylodes
macrocephala
(X1)

Salvia
miltiorrhiza
(X2)

Citrus
aurantium
(X3)

Paeonia
lactiflora
(X4)

Pueraria
lobata
(X5)

Alisma
orientale
(X6)
Schisandra
chinensis
(X7)

Curcuma
longa
(X8)

Group 1

0.830

1.830

1.170

2.170

1.330

0.670

1.000
1.500

Group 2

1.000

2.500

0.670

1.830

1.330

0.830

0.500

1.170

Group 3

1.170

1.830

1.500

1.330

1.500

1.170

1.330

0.830

Group 4

1.330

2.500

0.830

2.330

1.500

1.330

0.830

0.500

Group 5

1.500

1.670

1.670

2.000

1.670

1.670

0.330

1.500

Group 6

1.670

2.330

1.170

1.500

1.670

0.500

1.330

1.170

Group 7

1.830

1.670

0.500

2.500

1.830

0.670

0.833

0.830

Group 8

2.000

2.330

1.330

2.170

1.830

1.000

0.330

0.500

Group 9

0.830

1.500

0.830

1.670

2.000

1.170

1.170

1.670

Group 10

1.000

2.170

1.670

1.330

2.000

1.500

0.670

1.330

Group 11

1.170

1.500

1.000

2.330

2.170

1.670

0.170

1.000

Group 12

1.330

2.170

0.500

1.830

2.170

0.500

1.170

0.670

Group 13

1.500

1.330

1.330

1.500

2.330

0.830

0.670

1.670

Group 14

1.670

2.000

0.670

2.500

2.330

1.000

0.170

1.330

Group 15

1.830

1.330

1.500

2.000

2.500

1.330

1.000

1.000

Group 16

2.000

2.000

1.000

1.670

2.500

1.500

0.500

0.670

Table 2

Body weight of rats before killed and their total food-intake of each group (x±s)"


Group

n

Body weight (g)

Food-intake (L)

Lieber-DeCarli control diet

5

241.20±14.67

1.933±0.053

Lieber-DeCarli ethanol diet

4

242.75±28.83

1.935±0.181

Group 1

5

246.60±20.78

1.921±0.096

Group 2

5

233.00±27.02

1.913±0.166

Group 3

5

240.80±27.91

1.965±0.230

Group 4

3

237.67±30.62

1.890±0.223

Group 5

4

236.75±27.91

1.938±0.171

Group 6

5

242.80±2.59

1.924±0.055

Group 7

5

241.80±20.19

1.928±0.137

Group 8

4

249.75±28.69

1.935±0.238

Group 9

2

238.00±9.90

1.902±0.038

Group 10

3

242.00±28.50

1.955±0.182

Group 11

4

226.50±15.97

1.907±0.144

Group 12

5

236.40±30.33

1.928±0.260

Group 13

5

238.00±24.36

1.970±0.199

Group 14

5

239.20±21.37

1.930±0.109

Group 15

3

224.33±17.01

1.948±0.096

Group 16

4

242.50±31.86

1.940±0.201

Table 3

Content of plasma endotoxin of portal vein in each group tested by uniform design (x±s, EU/mL)"


Group of uniform design

n

Plasma endotoxin

Group 1

5

0.33±0.19

Group 2

5

0.36±0.05

Group 3

5

0.54±0.22

Group 4

3

0.44±0.38

Group 5

4

0.44±0.18

Group 6

5

0.26±0.16

Group 7

5

0.39±0.30

Group 8

4

0.32±0.13

Group 9

2

0.55±0.53

Group 10

3

0.21±0.12

Group 11

4

0.19±0.10

Group 12

5

0.40±0.39

Group 13

5

0.16±0.10

Group 14

5

0.27±0.08

Group 15

3

0.57±0.22

Group 16

4

0.09±0.03

Table 4

Body weight before killed and total food-intake of each group (x±s)"


n

Body weight (g)

Food-intake (mL)

Lieber-DeCarli control diet

10

256.40±9.67

2 029.00±84.71

Lieber-DeCarli ethanol diet

10

261.50±14.12

2 000.00±104.06

Jianpi Huoxue Decoction

9

261.00±17.90

1 992.22±121.47

Major herbs

9

260.78±13.14

1 996.11±119.23

Table 5

Content of plasma endotoxin of portal vein in each group (x±s, EU/mL)"


Group

n

Plasma endotoxin

Normal

10

0.21±0.18

Lieber-DeCarli control diet

10

0.48±0.52

Lieber-DeCarli ethanol diet

10

1.40±1.06**

Jianpi Huoxue Decoction

9

0.52±0.27△△

Major herbs

9

0.36±0.37△△

Figure 1

Pathological changes of liver of rats in each group under a light microscope (hematoxylin and eosin staining, ×200) N: Normal group; C: Lieber-DeCarli control diet group; M: Lieber-DeCarli ethanol diet group; J: Jianpi Huoxue Decoction group; H: Major herbs group."

Figure 2

Pathological changes of intestine of rats in each group under a light microscope (hematoxylin and eosin staining, ×100) N: Normal group; C: Lieber-DeCarli control diet group; M: Lieber-DeCarli ethanol diet group; J: Jianpi Huoxue Decoction group; H: Major herbs group."

Figure 3

Ultrastructure of intestine of rats in each group under an electron microscope (×11 500) N: Normal group; C: Lieber-DeCarli control diet group; M: Lieber-DeCarli ethanol diet group; J: Jianpi Huoxue Decoction group; H: Major herbs group."

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