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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (4): 427-431.doi: 10.3736/jcim20070413

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shenfu Injection on prostacyclin, thromboxane A2 and activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury

Song-lin Peng1, Xi Gu1, Chao-liu Dai1, Yong Huang2, Yong Zhao1   

  1. 1. Department of Hepatobiliary and Mammary Surgery, The Second Hospital, China Medical University, Shenyang, Liaoning Province 110004, China  2. Department of General Surgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong Province 271100, China
  • Online:2007-07-31 Published:2007-05-15

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Objective: To explore the effects of Shenfu Injection on prostacyclin, thromboxane A2 and the activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury.Methods: Twenty-four male Wistar rats weighing 200-250 g were randomly divided into two groups: Shenfu Injection (SF)-treated group (rats were treated with Shenfu Injection of 10 ml/kg through intraperitoneal injection) and untreated group (rats were administered with normal saline at the same dose and served as a control group). Hepatic ischemia was caused by Pringle's maneuver and lasted for fifteen minutes, and then one-hour or three-hour reperfusion was performed. Venous blood samples for the measurement of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) were collected three hours after reperfusion. Liver tissue samples were collected one hour or three hours after reperfusion for the measurement of Na+-K+-ATPase and Ca2+-Mg2+-ATPase and for morphological studies.Result: Plasma TXB2 was lower in the SF-treated group than that in the untreated group after three-hour reperfusion (P>0.05), while 6-keto-PGF1α was higher in the SF-treated group than that in the untreated group (P>0.05). The ratio of TXB2 and 6-keto-PGF1α was significantly lower in the SF-treated group than that in the untreated group (P＜0.05). The activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in the SF-treated group were improved obviously. A three-hour reperfusion after fifteen-minute ischemia caused important hepatic histological alterations. Marked structural abnormalities were observed in the untreated group, such as massive hepatocyte swelling, necrosis, mitochondria edema and vacuolar changes. In the SF-treated group, hepatic tissue injury was reduced significantly.Conclusion: Shenfu Injection protects hepatic tissue from ischemia-reperfusion injury, and such protective effects are achieved by decreasing the ratio of thromboxane A2 and prostacyclin, and increasing the activities of Na+-K+-ATPase and Ca2+-Mg2+- ATPase.

Key words: ischemia-reperfusion injury, thromboxane A2, prostacyclin, Na+-K+-ATPase, Ca2+-Mg2+-ATPase

CLC Number: 

  • R657.3

Table 1

Plasma concentrations of TXB2 and 6-keto-PGF1α and their ratio after three-hour reperfusion（ヌ±S）"

Groupn TXB2（ng/L）6-keto-PGF1a（ng/L）TXB2/6-keto-PGF1a
Untreated6386.3±282.7960.0±209.90.39±0.24
SF-treated6118.7±19.11 081.7±282.70.11±0.03*

Table 2

Levels of hepatic tissue ATPases after one- or three-hour reperfusion[ヌ±S, μmol Pi/(mg prot·h)]"

Groupn1 h3 h
Na+-K+-ATPaseCa2+-Mg2+-ATPaseNa+-K+-ATPaseCa2+-Mg2+-ATPase
Untreated67.76±1.757.37±1.477.84±1.077.43±0.65
SF-treated610.48±1.56*9.39±0.96*11.54±2.28*10.99±2.93*

Figure 1

Massive hepatocyte swelling, necrosis, sinusoidal and microvascular congestion in untreated group (HE staining, ×100)"

Figure 2

Sinusoidal congestion and tissue injury were reduced significantly in SF-treated group (HE staining, ×100)"

Figure 3

Severe injuries of hepatocytes and mitochondria in untreated group (transmission electron microscope, ×8 000)"

Figure 4

Injuries of hepatocytes and mitochondria were reduced in SF-treated group (transmission electron microscope, ×8 000)"

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