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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (10): 1054-1058.doi: 10.3736/jcim20081013

• Original Experimental Research • Previous Articles     Next Articles

Chaiqin Chengqi Decoction decreases pancreatic acinar cell calcium overload in rats with acute pancreatitis

Ping Xue1, Li-hui Deng1, Zhao-da Zhang2, Xiao-nan yang1, Qing Xia1(), Da-kai Xiang1, Lei Huang1, Mei-hua Wan1, Hai-yan Zhang1   

  1. 1. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, China
    2. Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, China
  • Received:2008-02-26 Online:2008-10-20 Published:2008-10-15

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Objective: 

To investigate the mechanism of Chaiqin Chengqi Decoction (CQCQD), a compound of traditional Chinese herbal medicine, acting on the pancreatic acinar cell calcium overload in rats with acute pancreatitis (AP).


Methods:

A total of 30 SD rats were randomly divided into normal control group, untreated group and CQCQD group (n=10, respectively). AP was induced in rats by caerulein (5×50 μg/kg) intraperitoneal injection within 4 h. The pancreatic tissue SERCA1 and SERCA2 mRNA expressions were detected by fluorescent quantization polymerase chain reaction method; intracellular calcium fluorescence intensity (FI) of pancreatic acinar cells and the pancreatic pathological score were measured by laser scanning confocal microscopy and light microscopy respectively.


Results: 

There were no SERCA1 mRNA expressions in pancreatic acinar cells of rats in the normal control group and the untreated group. The expression of pancreatic SERCA2 mRNA in the untreated group was down-regulated compared with that in the normal control group (expression ratio=0.536; P=0.001); the expression of pancreatic SERCA2 mRNA in the CQCQD group was up-regulated compared with that in the untreated group (expression ratio=2.00; P=0.012). The pancreatic pathological score in the CQCQD group was lower than that in the untreated group and the FI of Ca 2+ was also lower.


Conclusion:

CQCQD can up-regulate the expression of pancreatic SERCA2 mRNA, release the calcium overload, and hence reduce the pathological changes in pancreatic tissue.

Key words: Chaiqin Chengqi Decoction, acute pancreatitis, calcium overload, sarcoendoplasmic reticulum Ca 2+-ATPase

CLC Number: 

  • R289.5

Table 1

Pancreatic pathological score and calcium fluorescence content in pancreatic acinar cells of rats in the three groups（x±s）"

Group n Pathological score Calcium FI
Normal control 10 0.8±1.0 111.0±18.4
Untreated 10 9.2±2.7* 138.2±23.1*
CQCQD 10 5.7±1.9△* 118.7±15.2△

Figure 1

Agar gel electrophoresis photograph of PCR products of SERCA1 mRNA A: GAPDH; B: Skeletal muscle control; C:Normal control group; D: Untreated group; E: CQCQD group."

Table 2

Cycle threshold value of GAPDH and SERCA2 mRNA in pancreatic acinar cells of rats in the three groups(x±s)"

Group n GAPDH SERCA2 mRNA
Normal control 10 19.35±0.28 21.50±0.50
Untreated 10 19.25±0.57 22.30±0.79*
CQCQD 10 18.50±0.22 20.7±0.18△

Figure 2

Agar gel electrophoresis photograph of PCR products of SERCA2 mRNA A: GAPDH gene in CQCQD group; B: SERCA2 mRNA in CQCQD group; C: GAPDH gene in untreated group; D: SERCA2 mRNA in untreated group; E: GAPDH gene in normal control group; F: SERCA2 mRNA in normal control group."

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