Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (6): 658-664.doi: 10.3736/jcim20110613

Special Issue: Acupuncture & Moxibustion

• Original Experimental Research • Previous Articles     Next Articles

Effects of electroacupuncture at Tianshu (ST25) on pro- and anti-inflammatory cytokines in rats with severe acute pancreatitis

Qi-ming Xue, Lu Huang, Ning Li()   

  1. Department of Integrated Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2011-03-05 Accepted:2011-04-07 Online:2011-06-20 Published:2011-06-15
  • Contact: Li Ning E-mail:zhenjiuhuaxi@163.cm

Objective: To observe the effects of electroacupuncture (EA) at Tianshu (ST25) on pro- and anti-inflammatory cytokines in sodium taurocholate-induced severe acute pancreatitis (SAP) in rats.
Methods: Sixty-six male Sprague-Dawley rats were randomly divided into three groups: sham-operation group, SAP group and EA group. An SAP model was established by injecting 3.5% sodium taurocholate at a dose of 1 mL/kg through puncturing the pancreatic duct. In the EA group, EA at ST25 was performed with sparse-dense wave (2/100 Hz, 2 mA for 30 min) immediately after sodium taurocholate injection and 30 min before sacrifice. Rats were sacrificed at 3 (n=7), 6 (n=7) and 12 h (n=8) after operation. Levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-10 in serum were estimated by enzyme-linked immunosorbent assay. Pathological changes of pancreatic tissues were examined by hematoxylin and eosin staining and observed under a microscope and also scored.
Results: The levels of serum TNF-α and IL-6 of SAP rats were significantly higher than those of sham-operation rats at 3, 6 and 12 h. The level of IL-10 in serum of SAP rats was significantly higher than that of sham-operation rats at 3 and 6 h (P<0.05). EA at ST25 down-regulated the levels of TNF-α and IL-6 in serum and up-regulated the level of IL-10 and the ratio of IL-10/TNF-α, also attenuated the morphological damages at 6 and 12 h (P<0.05) in rats with SAP. The pathological scores of pancreatic tissues in the EA group were significantly less than those in the SAP group at 6 and 12 h (P<0.05).
Conclusion: EA at ST25 may have a therapeutic effect on rats with SAP by down-regulating the levels of TNF-α and IL-6 and up-regulating the level of IL-10 to re-establish the balance of pro- and anti-inflammatory cytokines, and to ameliorate the inflammatory response of SAP.

Key words: severe acute pancreatitis, point ST25 (Tianshu), tumor necrosis factor-a, interleukin-6, interleukin-10, electroacupuncture, rats

"

"

"

"

"

Figure 1

Pathological changes of pancreatic tissues of rats in different groups observed by hematoxylin and eosin staining (Light microscopy, ×200) A-C: Sham-operation group; D-F: Severe acute pancreatitis group; G-I: Electroacupuncture group."

[1] American Gastroenterological Association( AGA) Institute on “Management of Acute Pancreatits” Clinical Practice and Economics Committee; AGA Institute Governing Board.AGA Institute medical position statement on acute pancreatitis[J]. Gastroenterology, 2007,132(5):2019-2021
doi: 10.1053/j.gastro.2007.03.066 pmid: 17484893
[2] Liu XB, Jiang JM, Huang ZW, Tian BL, Hu WM, Xia Q, Chen GY, Li QS, Yuan CX, Luo CX, Yan LN, Zhang ZD . Clinical study on the treatment of severe acute pancreatitis by integrated traditional Chinese medicine and Western medicine[J]. Sichuan Da Xue Xue Bao Yi Xue Ban, 2004,35(2):204-208
doi: 10.3969/j.issn.1672-173X.2004.02.017
刘续宝, 蒋俊明, 黄宗文, 田伯乐, 胡伟明, 夏庆, 陈光远, 李全生, 袁朝新, 罗传新, 严律南, 张肇达 . 中西医结合治疗重症急性胰腺炎的临床研究(附1376例报告)[J]. 四川大学学报(医学版), 2004,35(2):204-208
doi: 10.3969/j.issn.1672-173X.2004.02.017
[3] Xia Q, Huang ZW, Jiang JM, Chen GY, Yang XN, Tang WF, Zhang ZD, Liu XB, Hu WM, Tian BL, Li QS, Yan LN, Luo CX, Kang Y, Yuan CX, Song B, Luo Y . “Yi-Huo-Qing-Xia” method as the main therapy in integrated traditional Chinese and Western medicine on severe acute pancreatitis: a report of 1 161 cases[J]. Zhongguo Zhong Xi Yi Jie He Ji Jiu Za Zhi, 2006,13(3):131-134
夏庆, 黄宗文, 蒋俊明, 陈光远, 杨晓楠, 唐文富, 张肇达, 刘续宝, 胡伟明, 田伯乐, 李全生, 严律南, 罗传兴, 康焰, 袁朝新, 宋彬, 罗燕 . 以“益活清下”为主的中西医结合综合疗法治疗重症急性胰腺炎1 161例疗效报告[J]. 中国中西医结合急救杂志, 2006,13(3):131-134
[4] Wang XY, Shi X, He L . Effect of electroacupuncture on gastrointestinal dynamics in acute pancreatitis patients and its mechanism[J]. Zhen Ci Yan Jiu, 2007,32(3):199-202
doi: 10.3969/j.issn.1000-0607.2007.03.014
王新宇, 石现, 何磊 . 电针对急性胰腺炎患者胃肠动力的影响及其机制研究[J]. 针刺研究, 2007,32(3):199-202
doi: 10.3969/j.issn.1000-0607.2007.03.014
[5] Kim CD . Pancreatitis — etiology and pathogenesis[J]. Korean J Gastroenterol, 2005,46(5):321-332
pmid: 16301844
[6] Aho HJ, Koskensalo SM, Nevalainen TJ . Experimental pancreatitis in the rats. Sodium taurocholate-induced acute hemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980,15(4):411-416
doi: 10.3109/00365528009181493
[7] Lin WZ, Wang P. Experimental acupuncturology[M]. Shanghai: Shanghai Scientific and Technical Publishers, 1994: 286-290
林文注, 王佩 . 实验针灸学[M]. 上海: 上海科学技术出版社, 1994: 286-290
[8] Hua XB, Zhou HL . Development of the acupuncture point map of rats[J]. Shi Yan Dong Wu Yu Dong Wu Shi Yan, 1991,3(1):1-5
华兴邦, 周浩良 . 大鼠穴位图谱的研制[J]. 实验动物与动物实验, 1991,3(1):1-5
[9] Wang ZJ, Li WM . Effects of electroacupuncture on disorder of intestinal motility in a rat model of irritable bowel syndrome[J]. J Chin Integr Med, 2010,8(9):883-887
王智君, 李为民 . 电针对肠易激综合征大鼠肠道运动异常的调节作用[J]. 中西医结合学报, 2010,8(9):883-887
[10] Schmidt J, Rattner DW, Lewandrowski K, Compton CC, Mandavilli U, Knoefel WT, Warshaw AL . A better model of acute pancreatitis for evaluating therapy[J]. Ann Surg, 1992,215(1):44-56
doi: 10.1097/00000658-199201000-00007 pmid: 1731649
[11] Wang CW, Li N, He HB, Lü JQ, Liu ZS . Effect of electroacupuncture of Tianshu(ST25) on the rational symptoms of functional constipation patients and evaluation on its efficacy satisfaction: A single center, prospective, practical and randomized control trial[J]. Zhen Ci Yan Jiu, 2010,35(5):375-379
王成伟, 李宁, 何洪波, 吕建琴, 刘志顺 . 电针双侧天枢穴对功能性便秘患者自觉症状的影响及疗效满意度评价: 一项单中心、前瞻性随机对照临床试验[J]. 针刺研究, 2010,35(5):375-379
[12] Liu XQ, Liu W . Observation on therapeutic effect of acupuncture on paralytic intestinal obstruction after operation[J]. Zhongguo Zhen Jiu, 2004,24(12):831-832
doi: 10.3969/j.issn.1005-0779.2004.09.010
刘学岐, 刘玮 . 针刺治疗术后麻痹性肠梗阻疗效观察[J]. 中国针灸, 2004,24(12):831-832
doi: 10.3969/j.issn.1005-0779.2004.09.010
[13] Bhatia M, Wong FL, Cao Y, Lau HY, Huang J, Puneet P, Chevali L . Pathophysiology of acute pancreatitis[J]. Pancreatology, 2005,5(2-3):132-144
doi: 10.1159/000085265
[14] Bhatia M, Brady M, Shokuhi S, Christmas S, Neoptolemos JP, Slavin J . Inflammatory mediators in acute pancreatitis[J]. J Pathol, 2000,190(2):117-125
doi: 10.1002/(ISSN)1096-9896
[15] Pooran N, Indaram A, Singh P, Bank S . Cytokines(IL-6, IL-8, TNF): early and reliable predictors of severe acute pancreatitis[J]. J Clin Gastroenterol, 2003,37(3):263-266
doi: 10.1097/00004836-200309000-00013
[16] Gomez-Cambronero LG, Sabater L, Pereda J, Cassinello N, Camps B, Vina J, Sastre J . Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications[J]. Curr Drug Targets Inflamm Allergy, 2002,1(4):393-403
doi: 10.2174/1568010023344544
[17] Jiang CF, Shiau YC, Ng KW, Tan SW . Serum interleukin-6, tumor necrosis factor α and C-reactive protein in early prediction of severity of acute pancreatitis[J]. J Chin Med Assoc, 2004,67(9):442-446
[18] Xia J, Ding YM . Significance on systemic inflammatory response syndrome and changes of serum cytokines in early stage of acute pancreatitis[J]. Yi Xian Bing Xue, 2002,2(3):162-165
夏菁, 丁一民 . 急性胰腺炎早期全身炎症反应综合征和细胞因子变化的意义[J]. 胰腺病学, 2002,2(3):162-165
[19] Ramudo L, Manso MA, Vicente S, De Dios I . Pro- and anti-inflammatory response of acinar cells during acute pancreatitis.Effect of N-acetyl cysteine[J]. Cytokine, 2005,32(3-4):125-131
doi: 10.1016/j.cyto.2005.07.017
[20] Ohmoto K, Yamamoto S . Serum interleukin-6 and interleukin-10 in patients with acute pancreatitis: clinical implications[J]. Hepatogastroenterology, 2005,52(64):990-994
[21] Rongione AJ, Kusske AM, Kwan K, Ashley SW, Reber HA , McFadden DW.Interleukin 10 reduces the severity of acute pancreatitis in rats[J]. Gastroenterology, 1997,112(3):960-967
doi: 10.1053/gast.1997.v112.pm9041259 pmid: 9041259
[22] Zhang XP, Chen L, Hu QF, Tian H, Xu RJ, Wang ZW, Wang KY, Cheng QH, Yan W, Li Y, Li QY, He Q, Wang F . Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis[J]. World J Gastroenterol, 2007,13(41):5506-5511
doi: 10.3748/wjg.v13.i41.5506
[23] Dang SC, Zhang JX, Qu JG, Mao ZF, Wang XQ, Zhu B . Dynamic changes of IL-2/IL-10, sFas and expression of Fas in intestinal mucosa in rats with acute necrotizing pancreatitis[J]. World J Gastroenterol, 2008,14(14):2246-2250
doi: 10.3748/wjg.14.2246
[24] Li H, Li C, Du SH, Li YW, Chen DF . Influence of electro-acupuncture of Neiguan on plasmic concentrations of NO and TNFα in endotoxin shock rats[J]. J Chin Integr Med, 2003,1(4):281-284
黎晖, 李春, 杜少辉, 李伊为, 陈东风 . 电针内关对内毒素休克大鼠血浆一氧化氮及肿瘤坏死因子α浓度的影响[J]. 中西医结合学报, 2003,1(4):281-284
[25] Hu S, Zhang LJ, Bai HY, Bao CM . Effects of electro-acupuncture at Zusanli point on the expression of proinflammatory cytokines, the activity of diamine oxidase and the rate of water content in the small intestine in rats with sepsis[J]. Shi Jie Hua Ren Xiao Hua Za Zhi, 2009,17(20):2079-2082
doi: 10.3969/j.issn.1009-3079.2009.20.013
胡森, 张立俭, 白慧颖, 包呈梅 . 电针足三里对脓毒症大鼠小肠促炎症因子、二胺氧化酶活性及组织含水率的影响[J]. 世界华人消化杂志, 2009,17(20):2079-2082
doi: 10.3969/j.issn.1009-3079.2009.20.013
[1] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[2] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[3] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[4] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[5] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[6] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[7] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[8] Meng-yue Liu, Cheng-wei Wang, Zhou-peng Wu, Ning Li. Electroacupuncture for the prevention of postoperative gastrointestinal dysfunction in patients undergoing vascular surgery under general anesthesia: Study protocol for a prospective practical randomized controlled trial. Journal of Integrative Medicine, 2014, 12(6): 512-519.
[9] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[12] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[13] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[14] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
[15] Dilpesh Jain​, Manish Kumar Bansal, Rahul Dalvi​, Aman Upganlawar​, Rahul Somani​. Protective effect of diosmin against diabetic neuropathy in experimental rats. Journal of Integrative Medicine, 2014, 12(1): 35-41.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[4] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[5] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[6] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[7] Bing Hu , Hong-mei An , Ke-ping Shen , Qin Du. Senescence-inducing effects of Chinese herbal medicine Tenglong Buzhong Decoction on human colon carcinoma LS-174-T cells and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(11): 1048-1052
[8] Ya-bing Zhou, Jian-er Yu, Jie Wu, Li Bai, Li-li Huo, Xin-guang Zhang, Li-qing Li . Effects of Chinese herbal medicine Bushen Gubiao Recipe on toll-like receptor 4 and CD4 +CD25 +foxp3 +regulatory T cells in mice with recurrent respiratory tract infections. Journal of Chinese Integrative Medicine, 2010, 8(11): 1053-1059
[9] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[10] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100