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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (2): 185-189.doi: 10.3736/jcim20080216

• Original Experimental Research • Previous Articles     Next Articles

Effects of Yisui Jiedu Recipe on JAK2-STAT5 signal transduction pathway in bone marrow hematopoietic cells from patients with myelodysplastic syndrome-refractory anemia

Sheng-li Tian1(), Yong-ming Zhou2, Tao Huang2, Zhi-zhong Xue2, Wei He2   

  1. 1. Department of Hematology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Department of Hematology, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2007-04-11 Online:2008-02-20 Published:2008-02-15
  • Contact: TIAN Sheng-li E-mail:victorytian@126.com

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Objective: To investigate the effect of Yisui Jiedu Recipe (YSJDR), a compound traditional Chinese herbal medicine, on cytokines and their corresponding just another kinase 2-signal transducers and activators of transcription 5 (JAK2-STAT5) signal transduction pathway in bone marrow hematopoietic cells from patients with myelodysplastic syndrome-refractory anemia (MDS-RA).

Methods: Fluorogenic quantitative polymerase chain reaction (FQ-PCR) method was established to detect the levels of JAK2, STAT5 and Bcl-xL mRNA expressions, and JAK2-STAT5 signal transduction pathway was activated by granulocyte-macrophage-colony stimulating factor (GM-CSF) in cultured bone marrow hematopoietic cells from 10 patients with MDS-RA. The levels of interleukin-2 (IL-2), interleukin-3 (IL-3), γ-interferon (γ-INF) and tumor necrosis factor-α (TNF-α) in the cultural supernatant of untreated control, AG490-treated and YSJDF-treated cells were measured by enzyme-linked immunosorbent assay.

Results: The levels of IL-2 and TNF-α in YSJDR-treated group were significantly lower than those in untreated control group and AG490-treated group (P<0.01, P<0.05), and IL-3 level in YSJDP-treated group was remarkably higher than that in the other two groups (P<0.01). There were no significant differences in the levels of IL-2 and IL-3 between AG490-treated group and untreated control group (P>0.05), while the TNF-α level in AG490-treated group was decreased obviously as compared with the untreated control group (P<0.01). There was no significant difference in γ-INF level between YSJDR-treated group and AG490-treated group (P>0.05), while TNF-α level in the two groups were significantly lower than that in the untreated control group (P<0.01). The expressions of JAK2, STAT5 and Bcl-xL mRNAs were significantly down-regulated in the YSJDR-treated and the AG490-treated groups as compared with those in the untreated control group (P<0.05, P<0.01), while there were no differences in the expressions of JAK2, STAT5 and Bcl-xL mRNAs between YSJDR-treated group and AG490-treated group.

Conclusion: YSJDR can modulate cytokine level in bone marrow hematopoietic cells of MDS-RA, suppress JAK2-STAT5 signal transduction, and inhibit the Bcl-xL mRNA expression.

Key words: myelodysplastic syndromes, composite (traditional compound drug), cytokines, signal transduction

CLC Number: 

  • R556.5

Table 1

Levels of some cytokine expressions in different groups of cultured cells ($\bar{x}$±s, ng/L)"

Group n Level of cytokine expression
IL-2 IL-3 γ-INF TNF-α
Untreated control 10 24.64±18.50 42.19±44.42 62.71±38.92 66.65±25.58
AG490-treated 10 23.09±13.17 21.13±33.80 4.67±1.80** 17.12±14.65**
YSJDR-treated 10 8.12±6.55**△ 106.13±40.31**△△ 6.04±13.63** 10.78±19.40**△

Table 2

Signal gene mRNA expressions in three groups of cultured cells ($\bar{x}$±s)"

Group n JAK2 STAT5 Bcl-xL
Untreated control 10 5 693.31±3 372.60 1 697.70±598.03 1 198.46±539.96
AG490-treated 10 680.39±557.62** 58.37±40.26** 335.91±270.21**
YSJDP-treated 10 1 895.36±2 285.14** 378.91±467.82** 368.75±671.41*
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