川芎嗪注射液对高糖诱导后人腹膜间皮细胞Ⅰ型胶原、基质金属蛋白酶-1和金属蛋白酶组织抑制剂-1表达的影响

doi:10.3736/jcim20090110

Abstract

Objective

To investigate the effects of ligustrazine injection on type Ⅰ collagen, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) expressions in human peritoneal mesothelial cells (HPMCs) cultured in high glucose conditions.
Methods

HPMCs were isolated from human omenta by trypsin digestion method and subcultured. Then, the HPMCs were divided into normal control group, high glucose group and high glucose plus low-, medium- and high-dose ligustrazine (10, 20 and 40 mg/L ligustrazine respectively) groups. Semi-quantitative reverse transcription-polymerase chain reaction was used to detect the expressions of type Ⅰ collagen, MMP-1 and TIMP-1 mRNAs in HPMCs. Proteins of type Ⅰ collagen, MMP-1 and TIMP-1 in culture supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Cell protein concentration was measured by trace bicinchoninic acid method to correct the ELISA assay results.
Results

Ligustrazine injection could significantly decrease high glucose-induced type Ⅰ collagen and TIMP-1 expressions in a dose-dependent manner both in protein and gene levels (P<0.05, P<0.01). In addition, medium- and high-dose ligustrazine injection could significantly increase MMP-1 expression which was inhibited by high glucose concentrations (P<0.05).
Conclusion

Ligustrazine injection does not only decrease type Ⅰ collagen synthesis, but also promote its degradation by modulating unbalanced MMP-1/TIMP-1 expression in HPMCs cultured in high glucose conditions.

Key words: Ligustrazine injection, Human peritoneal mesothelial cells, High glucose, Type I collagen, Matrix metalloproteinase-1, Tissue inhibitor of metalloproteinase-1, In vitro

Gui-song Zhu, Jin-song He. Effects of ligustrazine injection on high glucose-induced type Ⅰ collagen, matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 expressions in human peritoneal mesothelial cells in vitro. Journal of Chinese Integrative Medicine, 2009, 7(1): 65-69.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090110

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References 12

[1]	Plum J, Hermann S, Fusshller A, Schoenicke G, Donner A, Rhrborn A, Grabensee B .Peritoneal sclerosis in peritoneal dialysis patients related to dialysis settings and peritoneal transport properties[J]. Kidney Int Suppl, 2001,( 78):S42-S47
[2]	Yung S, Liu ZH, Lai KN, Li LS, Chan TM . Emodin ameliorates glucose-induced morphologic abnormalities and synjournal of transforming growth factor beta1 and fibronectin by human peritoneal mesothelial cells[J]. Perit Dial Int, 2001,21(Suppl 3):S41-S47
[3]	Kim JJ, Li JJ, Kim KS, Kwak SJ, Jung DS, Ryu DR, Yoo TH, Choi HY, Han SH, Kim HJ, Yoon SY, Han DS, Kang SW . High glucose decreases collagenase expression and increases TIMP expression in cultured human peritoneal mesothelial cells[J]. Nephrol Dial Transplant, 2008,23(2):534-541
[4]	Perfumo F, Altieri P, Degl'Innocenti ML, Ghiggeri GM, Caridi G, Trivelli A, Gusmano R .Effects of peritoneal effluents on mesothelial cells in culture: cell proliferation and extracellular matrix regulation[J]. Nephrol Dial Transplant, 1996,11(9):1803-1809 doi: 10.1093/ndt/11.9.1803
[5]	Wang YD, Yan PY . Expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in ulcerative colitis[J]. World J Gastroenterol, 2006,12(37):6050-6053 doi: 10.3748/wjg.v12.i37.6050
[6]	Visse R, Nagase H . Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry[J]. Circ Res, 2003,92(8):827-839 doi: 10.1161/01.RES.0000070112.80711.3D
[7]	Fujisaki K, Tanabe N, Suzuki N, Mitsui N, Oka H, Ito K, Maeno M . The effect of IL-1alpha on the expression of matrix metalloproteinases, plasminogen activators, and their inhibitors in osteoblastic ROS 17/2.8 cells[J]. Life Sci, 2006,78(17):1975-1982 doi: 10.1016/j.lfs.2005.08.036
[8]	Huang Y, Chen SQ, Zhang G, Chen RH . Effect of tetromethylpyrazine and aminoguanidine on expression of vascular endothelial growth factor in kidney of diabetic rats[J]. J Chin Integr Med, 2004,2(1):39-41
	黄焱, 陈少强, 张更, 陈瑞华 . 川芎嗪联合氨基胍对糖尿病大鼠肾脏血管内皮细胞生长因子表达的影响[J]. 中西医结合学报, 2004,2(1):39-41
[9]	Lin YZ, Xu CX, Deng YL, Chen L, Huang H, Du J . Effects of tetramethylpyrazine on fibrosis of atrial tissue and atrial fibrillation in a canine model of congestive heart failure induced by ventricular tachypacing[J]. J Chin Integr Med, 2006,4(1):35-38
	林亚洲, 许春萱, 邓玉莲, 陈林, 黄海, 杜建 . 川芎嗪对心室快速起搏心力衰竭实验犬心房颤动及心房纤维化的影响[J]. 中西医结合学报, 2006,4(1):35-38
[10]	Hua HY, Li YY, Ge SW . Study of tetramethylpyrazine in treatment on liver fibrosis and mechanism in rats[J]. Zhong Yao Yao Li Yu Lin Chuang, 2007,23(5):60-62
	华海婴, 李艳瑛, 戈士文 . 川芎嗪抗大鼠免疫损伤性肝纤维化作用及机制研究[J]. 中药药理与临床, 2007,23(5):60-62
[11]	Lin YZ, Xu CX, Huang H . The mRNA expression of collagen Ⅰ, Ⅳ and TGF-β1 in canine atrium by prevention of tetramethylpyrazine during congestive heart failure[J]. Zhong Xi Yi Jie He Xin Nao Xue Guan Bing Za Zhi, 2005,3(6):512-514
	林亚洲, 许春萱, 黄海 . 川芎嗪干预心力衰竭实验犬心房组织Ⅰ、Ⅳ型胶原与TGF-β1的mRNA表达研究[J]. 中西医结合心脑血管病杂志, 2005,3(6):512-514
[12]	Li JW, Liu XZ, Liu XQ, Chen HR, Lin CH, Hu EX . Effect of ligustrazine on intimal proliferation and expression of tissue inhibitor of matrix metalloproteinase-1 in balloon-injured rabbit carotid artery[J]. Zhongguo Wei Qin Xi Shen Jing Wai Ke Za Zhi, 2004,9(7):314-317
	李敬文, 刘相轸, 刘晓谦, 陈会荣, 林成海, 胡恩喜 . 川芎嗪对颈动脉球囊损伤后基质金属蛋白酶组织抑制物-1基因表达的影响[J]. 中国微侵袭神经外科杂志, 2004,9(7):314-317

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Target gene		Primer sequence	Fragment size (bp)	PCR condition
Type Ⅰ collagen	forward	5'-TCC AAA GGA GAG AGC GGT AA-3'	693	95 ℃ 2 min, 94 ℃ 30 s, 56 ℃ 30 s, 72 ℃ 1 min, 72 ℃ 10 min, 30 cycles
	reverse	5'-GAC CAG GGA GAC CAA ACT CA-3'
MMP-1	forward	5'-CAT CCA AGC CAT ATA TGG ACG TTC C-3'	611	95 ℃ 1 min, 94 ℃ 30 s, 60 ℃ 1 min, 72 ℃ 1.5 min, 72 ℃ 10 min, 32 cycles
	reverse	5'-TCT GGA GAG TCA AAA TTC TCT TCG T-3'
TIMP-1	forward	5'-TGC ACC TGT GTC CCA CCC CAC CCA CAG ACG-3'	551	94 ℃ 3 min, 94 ℃ 50 s, 58 ℃ 50 s, 72 ℃ 1 min, 72 ℃ 10 min, 35 cycles
	reverse	5'-GGC TAT CTG GGA CCG CAG GGA CTG CCA GGT-3'
β-actin	forward	5'-TCG ACA ACG GCT CCG GCA T-3'	241	95 ℃ 3 min, 94 ℃ 40 s, 56 ℃ 30 s, 72 ℃ 1 min, 72 ℃ 10 min, 28 cycles
	reverse	5'-AAG GTG TGG TGC CAG ATT TTC-3'

Group	n	Type Ⅰ collagen	MMP-1	TIMP-1
Normal control	9	1.15±0.40	12.16±0.35	0.73±0.03
High glucose	9	6.63±0.08**	7.75±0.21**	6.69±0.27**
10 mg/L ligustrazine	9	5.99±0.26^△	7.90±0.42	4.98±0.06^△△
20 mg/L ligustrazine	9	4.46±0.28^△△	8.95±0.42^△△	3.97±0.09^△△
40 mg/L ligustrazine	9	3.40±0.24^△△	10.39±0.35^△△	3.24±0.03^△△

Effects of ligustrazine injection on high glucose-induced type Ⅰ collagen, matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 expressions in human peritoneal mesothelial cells in vitro

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