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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (2): 157-162.doi: 10.3736/jcim20080210

• Original Experimental Research • Previous Articles     Next Articles

Effects of active component in Cichorii on lipid metabolism of rat with hypertriglyceridemia complicated by hyperuricemia and hyperglycemia

Hui Li, Xiao-qing Liu, Bing Zhang(), Li Ni, Zhi-jian Ling   

  1. School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2007-04-16 Online:2008-02-20 Published:2008-02-15
  • Contact: ZHANG Bing

Objective:To observe the effect of active component in Cichorii (N3) on lipid metabolism of rat with hypertriglyceridemia complicated by hyperuricemia and hyperglycemia.

Methods:Hypertriglyceridemia complicated by hyperuricemia and hyperglycemia was induced in rats by feeding a kind of feedstuff containing high fructose for 21 days. Then three different doses of N3 were administered continuously to the rats for 35 days. The effects of N3 on lipid metabolism were observed by determining the levels of serum triglyceride (TG), uric acid (UA), glucose (GLU) and free fatty acids (FFA) and the activities of fatty acid synthetase (FAS) and hepatic lipase (HL) from the liver and lipoprotein lipase (LPL) from the heart.

Results:Twenty-one days after feeding a high-fructose diet, the levels of serum UA, TG and GLU in the untreated group were significantly higher than those of the normal control group. Thirty-five days after administering N3, there was no statistical difference in serum TG and heart LPL activity between the untreated group and the normal control group, while liver FAS activity, serum FFA level and liver HL activity in the untreated group were significantly higher than those in the normal control group. Different doses of N3 treated rats all showed a decrease of TG, UA and GLU levels. N3 could decrease FAS activity and FFA level, and increase LPL and HL activities significantly.

Conclusion:N3 in Cichorii may regulate the lipid metabolism of the rats with hypertriglyceridemia complicated by hyperuricemia and hyperglycemia by decreasing FAS activity and FFA level, and increasing LPL and HL activities.

Key words: Cichorii, hypertriglyceridemia, uric acid, hyperglycemia, rats

CLC Number: 

  • R589.2

Table 1

Changes of the levels of serum TG, UA and GLU in rats ($\bar{x}$±s)"

Group n Serum TG level (mmol/L) Serum UA level (μmol/L) Serum GLU level (mmol/L)
Day 10 Day 21 Day 10 Day 21 Day 10 Day 21
Normal control 10 1.35±0.24 1.22±0.21 64.20±20.38 52.69±8.61 2.15±0.66 3.79±0.39
Untreated 50 1.56±0.23* 1.76±0.42** 70.80±10.36 65.05±13.6* 1.65±0.7 4.55±0.63**

Table 2

Levels of serum TG, UA and GLU in rats at different administration time ($\bar{x}$±s)"

Group n TG (mmol/L) UA (μmol/L) GLU (mmol/L)
Normal control
7th day of administration 10 1.15±0.21 55.63±8.10 2.12±0.61
19th day of administration 10 1.00±0.17 48.84±7.42 2.86±0.71
35th day of administration 10 1.10±0.21 44.72±9.79 2.30±0.40
7th day of administration 10 1.69±0.36** 86.61±21.62** 2.78±0.41**
19th day of administration 10 1.62±0.32** 62.90±12.55** 3.65±0.72*
35th day of administration 10 1.95±0.39** 71.69±19.47** 2.93±0.72*
7th day of administration 10 1.27±0.12△△ 98.97±21.28 2.97±0.79
19th day of administration 10 1.54±0.25 123.10±27.45△△ 3.72±0.47
35th day of administration 10 1.22±0.23△△ 116.68±31.58△△ 3.50±0.70
High-dose N3-treated
7th day of administration 10 1.25±0.19△△ 112.67±51.23 2.59±0.79
19th day of administration 10 1.38±0.26 107.83±35.91△△ 1.79±0.70△△
35th day of administration 10 1.02±0.17△△ 76.68±20.03 2.10±0.73
Medium-dose N3-treated
7th day of administration 10 1.23±0.26△△ 90.86±25.43 2.70±0.81
19th day of administration 10 1.30±0.41 83.66±14.94△△ 2.31±0.53△△
35th day of administration 10 1.16±0.18△△ 53.28±18.41 2.13±0.71
Low-dose N3-treated
7th day of administration 10 1.32±0.23 87.99±32.01 2.42±0.78
19th day of administration 10 1.46±0.34 78.01±22.47 2.58±0.68△△
35th day of administration 10 1.29±0.49△△ 55.20±11.98 2.30±0.47

Table 3

Changes of serum FFA level, liver FAS, HL and heart LPL activities in rats on the 35th day of administration ($\bar{x}$±s)"

Group n Liver FAS activity
Serum FFA level
Heart LPL activity
Liver HL activity
Normal control 10 25.44±8.88 1 772.10±291.33 8.56±2.58 2.74±0.66
Untreated 10 37.36±7.92** 2 156.25±181.94** 8.45±2.22 1.98±0.24**
Fenofibrate-treated 10 28.47±6.56△△ 1 943.70±220.79 9.02±2.23 2.35±0.38
High-dose N3-treated 10 25.86±7.19 1 897.73±189.17 9.71±2.25 2.84±0.79△△
Medium-dose N3-treated 10 29.65±5.49 1 646.59±677.35 10.83±0.36△△ 2.56±0.59△△
Low-dose N3-treated 10 29.22±9.51 1 893.60±158.20△△ 10.75±0.67△△ 2.44±0.63

Table 4

Results of the levels of Ins and ISI in rats on the 35th day of administration ($\bar{x}$±s)"

Group n Ins (mU/L) ISI
Normal control 10 183.99±24.15 –(2.62±0.12)
Untreated 10 153.71±33.74* –(2.63±0.12)
Fenofibrate-treated 10 104.06±66.20* –(2.45±0.36)
High-dose N3-treated 10 145.75±72.34* –(2.46±0.47)
Medium-dose N3-treated 10 129.34±50.90* –(2.40±0.31)
Low-dose N3-treated 10 148.89±37.70* –(2.51±0.15)
[1] Hwang IS, Ho H, Hoffman BB , et al. Fructose- induced insulin resistance and hypertension in rats. Hypertension. 1987; 10(5):512-516.
doi: 10.1161/01.HYP.10.5.512 pmid: 3311990
[2] Zhang R, Liu Y , Liu BW. Colorimetric method for measuring activities of lipoprotein lipase and hepatic lipase in plasma. Hua Xi Yi Ke Da Xue Xue Bao. 1996; 27(1):106-110. Chinese.
张蓉, 刘宇, 刘秉文 . 血浆脂蛋白脂酶及肝脂酶的比色测定法. 华西医科大学学报. 1996; 27(1):106-110.
[3] Tian WX, Hu RY, Wang YS . Studies on the reactivity of the essential sulfhydryl groups as a conformational probe for the fatty acid synthetase of chicken liver. Inactivation by 5,5-dithiobis-( 2-nitrobenzoic acid) and intersubunit cross-linking of the inactivated enzyme. J BiolChem. 1985; 260(20):11375-11387.
[4] Tian WX, Dong Y, Quan H , et al. The dependence of fat level of hen on activity of fatty acid synthase in liver on different ages. Sheng Wu Hua Xue Za Zhi. 1996; 12(2):234-236. Chinese with abstract in English.
doi: 10.1088/0256-307X/13/12/022
田维熙, 董妍, 权晖 , 等. 不同生长期蛋鸡的体脂水平和肝脏脂肪酸合成酶活性的关系.生物化学杂志. 1996; 12(2):234-236.
doi: 10.1088/0256-307X/13/12/022
[5] Li GW, Pan XR, Step L , et al. A new insulin- sensitivity index for the population-based study. Zhong- hua Nei Ke Za Zhi. 1993; 32(10):656-660. Chinese with abstract in English.
李光伟, 潘孝仁 , Step L, 等. 检测人群胰岛素敏感性的一项新指数.中华内科杂志. 1993; 32(10):656-660.
[6] Lin KC, Lin HY, Chou P . Community based epidemiological study on hyperuricemia and gout in Kin Hu, Kinmen. J Rheumatol. 2000; 27(4):1045-1050.
doi: 10.1021/jp050364j pmid: 10782835
[7] Zhao SP. Study of clinical lipid. Changsha : Hunan Science and Technology Publishing House. 1997: 86-87. Chinese.
赵水平 . 临床血脂学.长沙: 湖南科学技术出版社. 1997: 86-87.
[8] Pan CY , Jin WS. Epidemiology of type 2 diabetes mellitus. Zhonghua Nei Fen Mi Dai Xie Za Zhi. 2005; 21(5) : supplement 5S-1-5S-5. Chinese.
doi: 10.3760/j.issn:1000-6699.2005.05.039
潘长玉, 金文胜 .2型糖尿病流行病学 .中华内分泌代谢杂志. 2005; 21(5):增录 5S"1-5S"5.
doi: 10.3760/j.issn:1000-6699.2005.05.039
[9] Pan CY. A new advance in the recognition and prevention of metabolic syndrome : comments on the new worldwide definition of metabolic syndrome, a consensus reached by International Diabetes Federation. Zhonghua Nei Fen Mi Dai Xie Za Zhi. 2005; 21(4):298-300. Chinese with abstract in English.
doi: 10.3760/j.issn:1000-6699.2005.04.003
潘长玉 . 代谢综合征认识和防治的新进展——评《国际糖尿病联盟关于代i射综合征定义的全球共识》.中华内分泌代谢杂志. 2005; 21(4):298-300.
doi: 10.3760/j.issn:1000-6699.2005.04.003
[10] Yan M, Lu RJ, Jia XQ , et al. Change and significance of serum free fatty acids of rat with hyperlipoidemia hepatic adipose. Shandong Yi Yao. 2001; 41(15) : 6-7. Chinese with abstract in English.
doi: 10.3969/j.issn.1002-266X.2001.15.003
闫明, 吕瑞娟, 贾晓青 , 等. 高脂血症性脂肪肝大鼠血清游离脂肪酸的变化及意义.山东医药. 2001; 41(15) : 6-7.
doi: 10.3969/j.issn.1002-266X.2001.15.003
[11] Lee RG . Nonalcoholic steatohepatitis : a study of 49 patients. Hum Pathol. 1989; 20(6):594-598.
doi: 10.1016/0046-8177(89)90249-9 pmid: 2656500
[12] Clare DA, Blakistone BA, Swaisgood HE , et al. Sulfhydryl oxidase-catalyzed conversion of xanthine dehydrogenase to xanthine oxidase. Arch Biochem Biophy. 1981; 211(1):44-47.
doi: 10.1016/0003-9861(81)90427-6 pmid: 694672721
[13] Song YL , Niu ZM. Study advancement of lipoprotein lipase. Zhongguo Xin Xue Guan Za Zhi. 1998; 3(5):388-391. Chinese.
宋艳丽, 牛振民 . 脂蛋白脂酶的研究进展.中国心血管杂志. 1998; 3(5):388-391.
[14] Wang HF, Wang RY. Hepatic lipase and artherosclero- sis. Guo Wai Yi Xue Nei Ke Xue Fen Ce. 2005; 32(7):285-288. Chinese.
王海芳, 王瑞英 . 肝脂酶与动脉粥样硬化.国外医学. 内科学分册. 2005; 32(7):285-288.
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