肾康灵汤剂联合强的松治疗阿霉素肾病大鼠的作用机制

doi:10.3736/jcim20090711

Abstract

Objective

To study the mechanism of Shenkangling (SKL), a compound traditional Chinese herbal medicine, combined with prednisone in treating adriamycin-induced nephropathy in rats.

Methods

Sixty-six SD male rats were randomly divided into normal control group, untreated group, prednisone group, SKL group and SKL plus prednisone group. Except the normal control group, rats were injected once via caudal vein with adriamycin (5.5 mg/kg) to induce nephropathy. Then, the rats were administered with prednisone, SKL, prednisone plus SKL or distilled water for 3 weeks, respectively. After harvest, 24-hour urine protein excretion, tumor necrosis factor-α (TNF-α) and nitric oxide (NO) contents in serum, and content of nuclear transcription factor-kappa B (NF-κB) p65 in mononuclear cells were determined, and correlation analysis among these parameters was performed. The content of NF-κB p65 was assayed with the patented method of Active Motif; TNF-α was assayed with enzyme-linked immunosorbent assay, and the content of NO was assessed by the method of nitrate reductase. The change of foot process in renal glomerulus was observed under an electron microscope.
Results

When the rats were administered with prednisone, SKL correspondingly, the contents of NF-κB p65, TNF-α and NO as well as 24-hour urine protein excretion were lower than those in the untreated group (P<0.01), and the fusion of foot process only recovered partially. Compared with other treated groups, the contents of NF-κB p65 and NO as well as 24-hour urine protein excretion were significantly decreased in SKL plus prednisone group (P<0.01, P<0.05), and the fusion of foot process recovered mostly also. There was no interaction between SKL and prednisone in decreasing the content of TNF-α.
Conclusion

Renal injury can be postponed and improved when treated with SKL puls prednisone; it may contribute to the depression of abnormal activation of NF-κB, and the inhibition of production of TNF-α and NO.

Key words: Adriamycin-induced nephropathy, Shenkangling, prednisone, NF-kappa B p65 subunit, Tumor necrosis factor-a, Nitric oxide, Rats

Qing Lin , Jian Zheng, Si Ai, Li-xing Lin, Yu-xin Chen, Fang Wang, Ling Zhu . Mechanism of combined treatment with Shenkangling Decoction and prednisone in rats with adriamycin-induced nephropathy. Journal of Chinese Integrative Medicine, 2009, 7(7): 661-666.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090711

http://www.jcimjournal.com/EN/Y2009/V7/I7/661

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References 12

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Prednisone	SKL	24-hour urine protein
Prednisone	SKL	n	Week 1	n	Week 2	n	Week 3
No	No	10	189.08±38.15	9	287.06±40.68	8	327.29±37.17
Yes	No	10	173.98±32.30	10	203.38±37.76	10	197.91±35.34
No	Yes	10	186.05±30.02	10	250.52±42.65	10	281.15±41.14
Yes	Yes	10	140.07±23.40	10	153.22±25.04	10	157.58±29.61

Prednisone	SKL	TNF-α
Prednisone	SKL	n	Week 1	n	Week 2	n	Week 3
No	No	10	15.01±3.91	9	20.50±3.81	8	23.91±3.46
Yes	No	10	12.63±2.27	10	12.06±2.48	10	11.57±2.80
No	Yes	10	13.89±2.64	10	17.22±3.63	10	17.62±3.28
Yes	Yes	10	11.92±2.06	10	10.09±1.74	10	7.41±1.86

Prednisone	SKL	NO
Prednisone	SKL	n	Week 1	n	Week 2	n	Week 3
No	No	10	81.10±12.46	9	97.81±10.71	8	114.80 10.66
Yes	No	10	75.14±11.33	10	84.10±8.80	10	59.90±10.86
No	Yes	10	81.43±9.78	10	91.09±7.07	10	85.21±8.79
Yes	Yes	10	72.60±11.05	10	62.34±11.97	10	50.07±8.66

Mechanism of combined treatment with Shenkangling Decoction and prednisone in rats with adriamycin-induced nephropathy

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