SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (4): 388-391.doi: 10.3736/jcim20060414

• Original Experimental Research • Previous Articles     Next Articles

Effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1

Tie Chong1, Jian-qiang Niu1, Zi-ming Wang1, Jun-jun She1, Chen Huang2   

  1. 1. Department of Urology, Second Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710004, China;
    2. Key Laboratory of Envi ronment and Genes Rela ted to Diseases , Xi’an Jiaotong Unive rsity , Minist ry of Education , Xi’an , Shaanxi Province 710004 , China
  • Online:2006-07-31 Published:2006-07-15

RichHTML

0

PDF (PC)

47

Like

Objective

To observe the effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1 in vitro, and to explore its mechanism.

Methods

The human renal cell carcinoma cell line GRC-1 was treated with matrine of different concentrations for 24, 48, 72 and 96 h respectively. The MTT assay was used to evaluate the cytotoxic effects of matrine on GRC-1 cells. The transmission electron microscope and flow cytometry were utilized to observe and detect the apoptosis of GRC-1 cells induced by matrine. The expression levels of Bcl-2 and Bax proteins were evaluated by streptavidin-biotin-peroxidase method.

Results

The matrine of different concentrations all have cytotoxic effects on GRC-1 cells, with obvious dose- and time-dependent effects. The apoptosis induced by matrine was confirmed in GRC-1 cells. With intervention of matrine (1.5g/L) for 12 h, the expression level of Bcl-2 in GRC-1 cells was decreased while the expression level of Bax was increased as compared with those in the untreated group.

Conclusion

The proliferation-inhibiting effects of matrine on human renal cell carcinoma cell line GRC-1 may be related to down-regulating the ratio of Bcl-2/Bax protein expression and promoting the apoptosis.

Key words: Matrine, Kidney neoplasms, Apoptosis

CLC Number: 

  • R737.11

Table 1

Growth-inhibiting effects of matrine on human renal cell carcinoma cell line GRC-1（ヌ±S, %）"

Concentration of matrine n Growth-inhibiting rate
24 h 48 h 72 h 96 h
0.3 g/L 5 6.3±5.7 8.8±3.2 14.9±12.8 18.4±12.0
0.5 g/L 5 11.5±6.6 12.3±9.6 21.9±6.5 22.4±2.0
0.8 g/L 5 20.5±7.7 23.1±8.1 25.4±9.0 36.2±4.8
1.0 g/L 5 23.6±6.1 25.4±7.4 29.2±9.5 45.8±2.9
1.5 g/L 5 31.6±4.2 32.0±5.5 34.0±4.9 60.2±2.1

Figure 1

Ultrastructure of human renal cell carcinoma cell line GRC-1 in different groups detected by transmission electron microscopeA: Untreated group; B: Matrine (1.0 g/L)-treated group (phase Ⅰ of apoptosis); C: Matrine (1.0 g/L)-treated group (phase Ⅱ of apoptosis)."

Figure 2

Classification percentage of human renal cell carcinoma cell line GRC-1 in different groups by AnnexinⅤ-FITC/PI staining assay and flow cytometryA: Untreated group; B: Matrine (0.5 g/L)-treated group; C: Matrine (1.0 g/L)-treated group; D: Matrine (1.5 g/L)-treated group."

Table 2

Expressions of Bcl-2 and Bax proteins in human renal cell carcinoma cell line GRC-1 in different groups（ヌ±S）"

Group n Bcl-2 protein Bax protein
Untreated 10 2.765±0.020 2.467±0.060
Matrine(1.5 g/L)-treated 10 2.655±0.005 2.699±0.020
t 12.84 10.82
P <0.01 <0.01
[1] Amato RJ . Renal cell carcinoma: review of novel single-agent therapeutics and combination regimens[J]. Ann Oncol, 2005,16(1):7-15
doi: 10.1093/annonc/mdi002 pmid: 15598929
[2] 司维柯, 李鹏, 王源 , 等. 苦参碱抗人肝癌细胞株HepG2的作用及其机制研究[J]. 国外医学·临床生物化学与检验学分册, 2003,24(5):288-290
doi: 10.3969/j.issn.1673-4130.2003.05.018
[3] 曾晖, 胡梅洁, 张曙 , 等. 苦参碱体外对胃癌细胞的杀伤作用[J]. 上海第二医科大学学报, 2004,24(1):38-40
[4] 彭彦辉, 郝玉宾, 史迎钦 , 等. 苦参碱对肠癌HT-29细胞株增殖的抑制作用及其机制[J]. 中华实验外科杂志, 2005,22(11):1353-1354
doi: 10.3760/j.issn:1001-9030.2005.11.028
[5] 赵亚群, 尚辉辉, 吴忠琪 , 等. 以顺铂为主方案加复方苦参注射液治疗晚期非小细胞肺癌[J]. 中国肺癌杂志, 2005,8(1):63-65
[6] 梁建新, 曾文铤, 朱科伦 , 等. 苦参碱联合介入化疗栓塞治疗中晚期肝癌疗效观察[J]. 广州医药, 2005,36(3):45-47
[7] 张德杰, 黄世林, 陈楠楠 , 等. 补骨脂素加长波紫外线照射对白血病K562细胞的杀伤作用[J]. 中西医结合学报, 2005,3(6):480-483
[8] Harrington DJ, Lessey BA, Rai V , et al. Tenascin is differentially expressed in endometrium and endometriosis[J]. J Pathol, 1999,187(2):242-248
[9] Oltvai ZN, Milliman CL, Korsmeyer SJ . Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death[J]. Cell, 1993,74(4):609-619
[1] Cai-lian Fana, Wan-jun Caib, Meng-nan Ye, Miao Chen, Yi Dai. Qili Qiangxin, a compound herbal medicine formula, alleviates hypoxia-reoxygenation-induced apoptotic and autophagic cell death via suppression of ROS/AMPK/mTOR pathway in vitro. Journal of Integrative Medicine, 2022, 20(4): 365-375.
[2] Hong-xiao Li, Ling Shi, Shang-jie Liang Chen-chen Fang, Qian-qian Xu, Ge Lu, Qian Wang, Jie Cheng, Jie Shen, Mei-hong Shen. Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway. Journal of Integrative Medicine, 2022, 20(2): 163-172.
[3] Mohamed S. Othman, Sofian T. Obeidat, Amal H. Al-Bagawi, Mohamed A. Fareid, Alaa Fehaid, Ahmed E. Abdel Moneim. Green-synthetized selenium nanoparticles using berberine as a promising anticancer agent#br#
#br#
. Journal of Integrative Medicine, 2022, 20(1): 65-72.
[4] Hyongjun Jeon, Hee-Young Kim, Chang-Hwan Bae, Yukyung Lee, Sungtae Koo, Seungtae Kim. Korean red ginseng decreases 1-methyl-4-phenylpyridinium-induced mitophagy in SH-SY5Y cells. Journal of Integrative Medicine, 2021, 19(6): 537-544.
[5] Porwornwisit Tritripmongkol, Tullayakorn Plengsuriyakarn, Mayuri Tarasuk, Kesara Na-Bangchang. In vitro cytotoxic and toxicological activities of ethanolic extract of Kaempferia galanga Linn. and its active component, ethyl-p-methoxycinnamate, against cholangiocarcinoma. Journal of Integrative Medicine, 2020, 18(4): 326-333.
[6] Varuni Colamba Pathiranage, Ira Thabrew, Sameera R. Samarakoon, Kamani H. Tennekoon, Umapriyatharshini Rajagopalan, Meran K. Ediriweera. Evaluation of anticancer effects of a pharmaceutically viable extract of a traditional polyherbal mixture against non-small-cell lung cancer cells. Journal of Integrative Medicine, 2020, 18(3): 242-252.
[7] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[8] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[9] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[10] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
[11] Bruno José Martins Da Silva, Sandro Wilson Gomes Pereira, Ana Paula Drummond Rodrigues, José Luiz Martins Do Nascimento, Edilene Oliveira Silva. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 2018, 16(6): 404-410.
[12] Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[13] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[14] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[15] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3