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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 541-545.doi: 10.3736/jcim20070513

• Original Experimental Research • Previous Articles     Next Articles

Effects of Huanglian Jiedu Decoction on phosphatidylinositol-3-kinase expression in target tissues of type 2 diabetic rats

Dan Jin1, Fu-er Lu1, Guang Chen1, Huan Sun1, Xiao-hong Lu2   

  1. 1.Department of Nephrology,Renji Hospital,Medical College of Shanghai Jiaotong University,Shanghai 200127,China
    2.Institute of Immunology,Medical College of Shanghai Jiaotong University,Shanghai 200025,China
  • Online:2007-09-20 Published:2007-09-15

Objective: To observe the effects of Huanglian Jiedu Decoction (HLJDD), a traditional Chinese compound herbal medicine, on p85 mRNA and protein expressions of phosphatidylinositol-3-kinase (PI-3K) in target tissues (skeletal muscular and adipose tissues) in rats with type 2 diabetes mellitus (T2DM) and to investigate the molecular mechanism of HLJDD in treating T2DM. Methods: The male Wistar rats were injected with streptozotocin (STZ) 30 mg/kg through tail vein, and fed with high-fat and high-caloric diets to induce T2DM. Then the rats were randomly divided into untreated group, aspirin-treated group and HLJDD group, and treated correspondingly. Meanwhile, a group of normal animals without any treatment was set up for normal control group. Ten weeks later, serum fasting blood glucose (FBG), serum fasting insulin (FINS) and oral glucose tolerance test (OGTT) were routinely determined. The expressions of PI-3K p85 mRNA and protein in skeletal muscle and adipose tissue were determined with RT-PCR and Western blotting before and after insulin treatment.Results: Compared with the untreated group, the FBG and OGTT levels in T2DM rats treated with HLJDD decreased significantly (P<0.05). The FINS in HLJDD group was lower than that in the normal control group (P<0.05), but was not significantly different from that in the untreated group. The PI-3K p85 mRNA and protein expressions in HLJDD group obviously increased, as compared with those in the untreated group.Conclusion: The effect of HLJDD in treating T2DM was probably associated with its improvement of PI-3K p85 mRNA and protein expressions in skeletal muscle and adipose tissue of the T2DM rats.

Key words: Huanglian Jiedu Decoction, diabetes mellitus , type 2, phosphatidylinositol-3-kinase, rats

CLC Number: 

  • R587.1

Table 1

FINS, FBG and OGTT in four groups (ヌ±S)"

GroupnFINS (mU/L)FBG (mmol/L)BG-0.5 h (mmol/L)BG-1 h (mmol/L)BG-2 h (mmol/L)
Normal control1233.31±3.863.39±0.195.91±0.226.84± 0.395.72±0.24

Figure 1

PI-3K p85 mRNA expression in skeletal muscle*P<0.05, vs untreated group."

Figure 2

PI-3K p85 mRNA expression in adipose tissue*P<0.05, vs untreated group."

Figure 3

PI-3K p85 protein expression in skeletal muscle*P<0.05, vs untreated group."

Figure 4

PI-3K p85 protein expression in adipose tissue*P<0.05, vs untreated group"

[1] 冷三华, 陆付耳, 屠庆年, 等.黄连解毒汤对2型糖尿病大鼠血糖和血脂代谢的影响[J].中国中医基础医学杂志,2003,9(4):43-45
1Leng SH, Lu FE, Tu QN, et al.Effects of Huang Lian Jie Du Decoction on blood glucose and lipids metabolisms in type Ⅱ diabetic rats[J].Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi,2003,9(4):43-45
[2] 叶爱丽, 陆付耳, 徐丽君, 等.黄连解毒汤对胰岛素抵抗大鼠骨骼肌IRS-1表达及其酪氨酸磷酸化水平的影响[J].中医杂志,2005,46(9):695-697
Ye AL, Lu FE, Xu LJ, et al.Effects of Huanglian Jiedu decoction on IRS-1 expression and tyrosine phosphorylation level in skeletal muscles of the rats with insulin resistance[J].Zhong Yi Za Zhi,2005,46(9):695-697
[3] 陈广, 陆付耳, 徐丽君.黄连解毒汤对2型糖尿病大鼠靶组织葡萄糖转运子4的影响[J].中西医结合学报,2007,5(4):412-415
Chen G, Lu FE, Xu LJ.Effects of Huanglian Jiedu Decoction on glucose transporter 4 in target tissues of type 2 diabetic rats[J].Zhong Xi Yi Jie He Xue Bao,2007,5(4):412-415
[4] 吴洪元, 姜晓群, 陈贤琼.黄连解毒胶囊提取工艺研究[J].中成药,1998,20(11):1-3
Wu HY, Jiang XQ, Chen XQ.Study on extraction process for Huanglianjiedu capsules[J].Zhong Cheng Yao,1998,20(11):1-3
[5] 5Yuan M, Konstantopoulos N, Lee J, et al.Reversal of obesity- and diet-induced insulin resistance with salicylates or targeted disruption of Ikkβ[J].Science,2001,293(5535):1673-1677
Yuan M, Konstantopoulos N, Lee J, et al.Reversal of obesity- and diet-induced insulin resistance with salicylates or targeted disruption of Ikkβ[J].Science,2001,293(5535):1673-1677
[6] 孙敬方. 动物实验方法学[M].北京: 人民卫生出版社,2001137-139, 197-212
Sun JF.Animal experiment technology[M].Beijing: People's Medical Publishing House,2001137-139,197-212
[7] Tremblay F, Lavigne C, Jacques H, et al.Dietary cod protein restores insulin-induces activation of phosphatidylinositol 3-kinase/Akt and GLUT4 translocation to the T-tubules in skeletal muscle of high-fat-fed obese rats[J].Diabetes,2003,52(1):29-37
[8] 陆付耳, 王智民, 郭爱群.糖尿病从“毒”论治探讨[J].中国中医基础医学杂志,2002,8(5):15-17
Lu FE, Wang ZM, Guo AQ.Exploration on the hypojournal of treating diabetes mellitus with the detoxification principle of traditional Chinese medicine[J].Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi,2002,8(5):15-17
[9] Lee YS, Kim WS, Kim KH, et al.Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states[J].Diabetes,2006,55(8):2256-2264
[10] Zhang CY, Parton LE, Ye CP, et al.Genipin inhibits UCP2-mediated proton leak and acutely reverses obesity-and high glucose-induced β cell dysfunction in isolated pancreatic islets[J].Cell Metab,2006,3(6):417-427
doi: 10.1016/j.cmet.2006.04.010
[11] Jackson S, Bagstaff S, Lynn S, et al.Decreased insulin responsiveness of glucose uptake in cultured human skeletal muscle cells from insulin-resistant nondiabetic relatives of type 2 diabetic families[J].Diabetes,2000,49(7):1169-1177
[12] Hundal RS, Petersen KF, Mayerson AB, et al.Mechanism by which high-dose aspirin improves glucose metabolism in type 2 diabetes[J].J Clin Invest,2002,109(10):1321-1326
doi: 10.1172/JCI14955 pmid: 12021247
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