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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (5): 570-572.doi: 10.3736/jcim20070519

• Original Experimental Research • Previous Articles     Next Articles

Effects of total saponins of Panax japonicus on human leukemic HL-60 cells

YUAN Ding1,2,ZUO Rui1,cheng ZHANG Chang1,2   

  1. 1. Medical College,Three Gorges University,Yichang,Hubei Province 443002, China
    2. Hubei Province Key Laboratory of Natural Products Research and Development,Three Gorges University,Yichang,Hubei Province 443002 ,China
  • Online:2007-09-20 Published:2018-10-25

Objective: To study the effects of total saponins of Panax japonicus (TSPJ) on human leukemic HL-60 cells.Methods: Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium (NBT) was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry.Results: Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G0/G1 phase in the different concentrations of TSPJ-treated groups were increased.Conclusion: TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.

Key words: Panax japonicus, HL-60 cells, cell differentiation

CLC Number: 

  • R285.5

Table 1

Effects of TSPJ on HL-60 cell proliferation"

Concentration (μg/ml) Inhibition rate in different action time (%)
12 h 24 h 48 h
12.5 2.01 15.34 25.36
25 4.32 24.55 37.59
50 9.21 18.41 25.90
100 7.52 35.56 61.87
200 15.60 40.07 66.73
400 20.00 42.06 66.19
800 74.44 84.12 90.83
1 600 75.56 86.10 92.09

Table 2

Effects of TSPJ on NBT reduction of HL-60 cells (ヌ±S )"

Group n NBT reduction rate
Negative control 5 6.9±1.5
100 μg/ml TSPJ-treated 5 37.9±1.3*
200 μg/ml TSPJ-treated 5 53.5±1.3*
[1] Yuan D, Zuo R, Zhang CC.Effects of total saponins of Panax japonicus(TSJP) on tumor growth in mice[J].Shi Zhen Guo Yi Guo Yao, 2007,18(2):277-278
袁丁,左锐,张长城.竹节参总皂苷抑制小鼠肿瘤细胞生长的实验研究[J].时珍国医国药, 2007,18(2):277-278
[2] Yuan D, Zuo R .Research on chemical constituents and biological activities of rhizoma of Panax japonicus[J].Shi Zhen Guo Yi Guo Yao, 2005,16( 9):838-839, 841
袁丁,左锐.竹节参化学成分和药理活性研究进展[J].时珍国医国药, 2005,16( 9):838-839, 841
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