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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 225-228.doi: 10.3736/jcim20050317

• Original Experimental Research • Previous Articles     Next Articles

Effects of Runing RecipeⅡ on expressions of p53 and ras oncogene proteins and cell cycle of the transplanted Ca761 breast cancer in mice

Qian-jun Chen1,Yi-an Wang1,Hong-lin Situ1,De-min Lu2,Li-ping Ren1,Xi-wen Lai1,Peng-xi Liu1,Yi Lin1   

  1. 1. Department of Breast Diseases, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510120, China
    2. Department of Breast Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-03-28 Online:2005-05-20 Published:2005-05-20
  • Contact: Qian-jun Chen E-mail:cqj55@163.com

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Objective

To explore the mechanisms of Runing RecipeⅡ (a recipe composed of traditional Chinese herbs) in inhibiting the growth of breast cancer by observing its effects on the expressions of p53 and ras oncogene proteins and cell cycle of the transplanted Ca761 breast cancer in mice.

Methods

We established the breast cancer model by transplanting Ca761 cells in mice. The mice were randomly divided into 4 groups: normal saline control group, CTX-treated group, Runing RecipeⅡ-treated group, and Runing RecipeⅡand CTX-treated group, with 12 mice in each group. We detected the cell cycle of the cancer cells in the mice’s transplanted tumor with flow cytometry and measured the expressions of p53 and ras oncogene proteins in the transplanted tumor with immunohistochemical methods.

Results

The percentages of tumor cells in S-phase of the Runing RecipeⅡ-treated group, CTX-treated group and Runing RecipeⅡand CTX-treated group were significantly lower than that of the normal saline control group respectively (P<0.05). The percentage of tumor cells in G0-G1 phase of the Runing RecipeⅡ-treated group was lower than that of the CTX-treated group (P<0.05), while the percentage of tumor cells in G2-M phase was higher than that of the CTX-treated group. The immunoreactive scores (IRSs) of p53 in the Runing RecipeⅡ-treated group and Runing RecipeⅡand CTX-treated group were significantly lower than that in the normal saline control group respectively (P<0.05). The effect of CTX on the expression of p53 was not significant. The IRSs of ras oncogene protein in the RecipeⅡ-treated group, CTX-treated group and Runing RecipeⅡand CTX-treated group were lower than that in the normal saline control group respectively (P<0.05).

Conclusion

Runing RecipeⅡ can inhibit the growth of Ca761 breast cancer in mice by controlling the cell cycle of the transplanted tumor. This may be related to its effect on the gene expressions of p53 and ras in the tumor tissue.

Key words: Chinese medicine and pharmacology, Breast cancer, Cell differentiation, Oncoproteins

CLC Number: 

  • R289.91

Tab 1

Percentages of cells in different cell cycles in 4 groups of mice with transplanted Ca761 breast cancer ($\bar{x}$±s, %)"

Group n G0-G1 phase S phase G2-M phase
Normal saline control 8 59.41±4.69 25.43±5.02 14.99±3.42
CTX-treated 8 74.10±4.98* 15.57±3.96* 10.33±3.72*
Runing RecipeⅡ-treated 8 66.25±5.19*△ 18.60±2.21*△ 15.16±4.45△
Runing RecipeⅡand CTX-treated 8 78.19±4.02* 13.03±6.05*△ 8.78±2.76*

Tab 2

Expressions of p53 and ras oncogene proteins in 4 groups of mice with transplanted Ca761 breast cancer ($\bar{x}$±s)"

Group n p53 IRS ras IRS
Normal saline control 10 5.60±2.76 4.70±1.41
CTX-treated 10 4.20±2.53 2.90±0.88*
Runing RecipeⅡ-treated 10 2.40±1.65* 2.70±1.42*
Runing RecipeⅡand CTX-treated 10 2.80±2.30* 2.80±1.03*
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