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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 64-67.doi: 10.3736/jcim20060117

• Original Experimental Research • Previous Articles     Next Articles

High-throughput screening assay for groups of effective components extracted from Xiaoxuming Recipe

Yue-hua Wang1, Hai-xia Zhang1, Qi Li2, Yi Ding3, Juan-juan Hu1, Guan-hua Du1   

  1. 1. Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
    2. Department of Biology, Beijing Forestry University, Beijing 100083, China
    3. Department of Pharmacy, Tsinghua University, Beijing 100084, China
  • Online:2006-01-20 Published:2006-01-15


To present a new approach for the research of effective components extracted from compounds of traditional Chinese medicine and their action mechanisms by high-throughput screening assay.


We observed the anti-oxidation activities of 240 sequential components (L1-L120 and A1-A120) extracted from Xiaoxuming Recipe, the effects of these components on SH-SY5Y cells with H2O2-induced and L-glutamic acid-induced damages and the levels of resting calcium ion in neurocytes of rats.


Some components (L1-L40, A100-A120) extracted from Xiaoxuming Recipe had the corresponding effects listed above. The combination of these components was regarded as the groups of effective components of Xiaoxuming Recipe in treating sequelae resulting from brain ischemia.


Xiaoxuming Recipe has protective effect on brain-ischemia-induced damages through the actions of multiple components with multiple targets.

Key words: High-throughput screening, Xiaoxuming Decoction, Brain ischemia

CLC Number: 

  • R285

Figure 1

Anti-oxidation activities of 240 sequential components extracted from Xiaoxuming Recipe Values represent means of three independent experiments."

Figure 2

Effects of 240 sequential components extracted from Xiaoxuming Recipe on viability of SH-SY5Y cells with H2O2-induced damageValues represent means of three independent experiments."

Figure 3

Effects of 240 sequential components extracted from Xiaoxuming Recipe on viability of SH-SY5Y cells with L-glutamic acid-induced damageValues represent means of three independent experiments."

Figure 4

Effects of 240 sequential components extracted from Xiaoxu-ming Recipe on levels of resting calcium ion in neurocytes of ratsValues represent means of three independent experiments."

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