Oxidative stress,Diabetes mellitus,type 1,Insulin,Ocimum gratissimum L.,Vernonia amygdalina L.," /> Oxidative stress,Diabetes mellitus,type 1,Insulin,Ocimum gratissimum L.,Vernonia amygdalina L.,"/> Comparison of antioxidant activity of insulin, <em>Ocimum gratissimum</em> L., and <em>Vernonia amygdalina</em> L. in type 1 diabetic rat model
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Journal of Integrative Medicine ›› 2017, Vol. 15 ›› Issue (4): 302-309.doi: 10.1016/S2095-4964(17)60332-7

Special Issue: Natural Drug

• Research Article • Previous Articles     Next Articles

Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model

Uduak Akpan Okon, Idorenyin Udo Umoren   

  1. Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Uyo, Akwa Ibom State 520001, Nigeria
  • Received:2016-11-14 Accepted:2017-01-04 Online:2017-07-15 Published:2017-07-15
  • Contact: Uduak Akpan Okon, PhD; E-mail: chairmo2013@gmail.com


Diabetes mellitus (DM) is known to be associated with increase of oxidative stress products. The direction of effect of any treatment on these products could therefore be a reliable measure of its efficacy on DM. So the aim of this study was to investigate the activity of insulin, Ocimum gratissimum L. (OG) and Vernonia amygdalina L. (VA) on oxidative stress products.


Thirty-six female Wistar rats weighing 150-200 g were randomly divided into six groups of six rats each. Thirty rats were induced for type 1 DM (DM1) with a single intraperitoneal administration of 65 mg/kg body weight of streptozotocin. Group 1 was normal control and was administered distilled water while Group 2 served as DM1 control group; Groups 3, 4, 5 and 6 were diabetic rats treated with 208 mg/kg OG (DM1 + OG), 52 mg/kg VA (DM1 + VA), 208 mg/kg OG + 52 mg/kg VA (DM1+OG +VA) and 0.16 IU insulin (DM1 + insulin) respectively. Determination of methemoglobin and sulfhemoglobin was achieved by the absorption spectrum principle. Red blood cell (RBC) catalase was assayed by continuous spectrophotometric method.


The RBC catalase concentration was significantly decreased in the DM1 and DM1+VA groups when compared with the normal control. DM1 + OG significantly increased RBC-catalase when compared to DM1. The methemoglobin concentration was significantly reduced in the DM1, DM1 + VA, DM1 + OG + VA and DM1 + insulin groups when compared to the normal control group. The sulfhemoglobin concentration was significantly increased in the diabetic control and the diabetic treated groups when compared to the normal control. DM1 + OG reduced the sulfhemoglobin concentration when compared to DM1. The blood glucose concentration of all the diabetic groups was significantly raised compared to normal control. OG, VA and insulin significantly reduced the blood glucose concentration with the efficacy of OG and VA higher than insulin.


Adverse alteration of oxidative indices were observed in type 1 DM model. Treatment with OG and insulin showed potent antioxidant activity, while the hypoglycemic efficacy of OG and VA were higher than insulin.

Key words: Oxidative stress, Diabetes mellitus, type 1, Insulin, Ocimum gratissimum L., Vernonia amygdalina L.

1 Okon U, Owu D, Udokang N, Udobang J, Ekpenyong C.Oral administration of aqueous leaf extract ofOcimum gratissimum ameliorates polyphagia, polydipsia and weight loss in streptozotocin-induced diabetic rats. Am J Med Sci. 2012; 2(3): 45-49.
2 Asuquo O, Edet A, Mesembe O, Atanghwo J.Ethanolic extracts ofVernonia amygdalina and Ocimum gratissimum enhance testicular improvement in diabetic Wistar rats. Int J Alt Med. 2009; 8(2): 432-440.
3 Egesie U, Adelaiye A, Ibu J, Egesie O.Safety and hypoglycaemic properties of aqueous leaf extract ofOcimum gratissimum in streptozotocin induced diabetic rats. Niger J Physiol Sci. 2006; 21(1-2): 31-35.
4 Fatmah A, Siti B, Zariyantey A, Nasar A, Jamaludin M.The role of oxidative stress and antioxidants in diabetic complications.Sultan Qaboos Univ Med J. 2012; 12(1): 5-18.
5 Giugliano D, Ceriello A, Paolisso G.Oxidative stress and diabetic vascular complications.Diabetes Care. 1996; 19(3): 257-267.
6 Pandey A, Tripathi P, Pandey R, Srivatava R, Goswami S.Alternative therapies useful in the management of diabetes: a systematic review.J Pharm Bioall Sci. 2011; 3: 504-512.
7 Guzik T, Mussa S, Gastaldi D, Sadowski J, Ratnatunga C, Pillai R, Channon K.Mechanisms of increased vascular superoxide production in human diabetes mellitus: role of NAD(P)H and endothelial nitric oxide synthase.Circulation. 2002; 105: 1656-1662.
8 Ergul A, Schultz J, Stromhang C, Harris A, Tawfik A, Wells B, Caldwell R.Vascular dysfunction of venous bypass conduits is mediated by reactive oxygen species in diabetes: role of endothelium-1.J Pharmacol Res Ther. 2004; 313: 70-77.
9 Amiri F, Shaw S, Wang X, Tang J, Waller J, Eaton D, Marrero M.Angiotensin II activation of JAK/STAT pathway in mesangial cells is altered by high glucose.Kidney Int. 2002; 61: 1605-1616.
10 Maritim A, Sanders R, Watkins J.Diabetes, oxidative stress and antioxidants: a review.J Biochem Mol Toxicol, 2003; 17: 24-38.
11 Shihana F, Dawson AH, Buckley NA.A bedside test for methemoglobinemia, Sri Lanka.Bull World Health Organ. 2016; 94(8): 622-625.
12 Sato K, Tamaki K, Okajima H, Katsumata Y.Longterm storage of blood samples as whole blood at extremely low temperature for methemoglobin determination.Forensic Sci Int. 1988; 37: 99-104.
13 Adams V, Marley J, McCarrol C. Prilocaine induced methemoglobinemia in a medically compromised patients. Was this an inevitable consequence of the dose administered?Br Dent J. 2007; 203(10): 585-587.
14 Flexman AM, Del Vicario G, Schwarz SK.Dark green blood in the operating theatre.Lancet. 2007; 369(9577): 1972.
15 Lorke D.A new approach to practical acute toxicity testing.Arch Toxicol. 1983; 54(4): 275-287.
16 Sofowora A.Medicinal plants and traditional medicine in Africa. 2nd ed. Ibadan: Spectrum Book Ltd. 1993.
17 Drabkin DL, Austin JR.Spectrophotometric studies. II.Preparations from washed blood cells; nitric oxide hemoglobin and sulfhaemoglobin. J Biol Chem 1935;112:51-65.
18 Maedler K, Sergeev P, Ris F, Oberholzer J, Joller-Jemelka HI, Spinas GA, Kaiser N, Halban PA, Donath MY.Glucose-induced β cell production of IL-1β contributes to glucotoxicity in human pancreatic islets.J Clin Invest. 2002; 110(6): 851-60.
19 Tanaka Y, Tran PO, Harmon J, Robertson RP.A role of glutathione peroxidase in protecting pancreatic β cells against oxidative stress in a model of glucose toxicity.Proc Natl Acad Sci U S A. 2002; 99: 12363-12368.
20 Gomathi D, Ravikumar G, Kalaiselvi M, Devaki K, Uma C.Efficacy ofEvolvulus alsinoides (L.) L. on insulin and antioxidants activity in pancreas of streptozotocin induced diabetic rats. J Diabetes Metab Disord. 2013; 12(1): 39.
21 Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK.Antidiabetic agents from medicinal plants.Curr Med Chem. 2006; 13(10): 1203-1218.
22 Arivazhagan P, Thilagavathy T, Pannerselvam C.Antioxidant lipoate and tissue antioxidants in aged rats.J Nutr Biochem. 2000; 11(3): 122-127.
23 Cheng L, Kellogg EW 3rd, Packer L. Photo inactivation of catalase.Photochem Photobiol. 1981; 34(1): 125-129.
24 Gaetani GF, Ferraris AM, Rolfo M, Mangerini R, Arena S, Kirkman HN.Predominant role of catalase in the disposal of hydrogen peroxide within human erythrocytes.Blood. 1996; 87(4): 1595-1599.
25 Shabeer J, Srivastava RS, Singh SK.Antidiabetic and antioxidant effect of various fractions ofPhyllanthus simplex in alloxan diabetic rats. J Ethnopharmacol. 2009; 124(1): 34-38.
26 Punitha R, Manoharan S.Antihyperglycemic and antilipidperoxidative effects ofPongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats. J Ethnopharmacol. 2006; 105(1-2): 39-46.
27 Vina J, Borras C, Gomez-Cabrera MC, Orr WC.Part of the series: from dietary antioxidants to regulators in cellular signalling and gene expression. Role of reactive oxygen species and (phyto)oestrogens in the modulation of adaptive response to stress.Free Radic Res. 2006; 40(2): 111-119.
28 Góth L, Lenkey A, Bigler WN.Blood catalase deficiency and diabetes in Hungary.Diabetes Care. 2001; 24(10): 1839-1840.
29 Góth L, Bigler NW.Catalase deficiency may complicate urate oxidase (rasburicase) therapy.Free Radic Res. 2007; 41(9): 953-955.
30 Góth L, Tóth Z, Tarnai I, Bérces M, Török P, Bigler WN.Blood catalase activity in gestational diabetes is decreased but not associated with pregnancy complications.Clin Chem. 2005; 51(12): 2401-2404.
31 Afolabi C, Akanimoladun E, Ibukun I, Emmanuel A, Obutor E, Foramb E.Phytochemical constituent and antioxidant activity of extract from the leaves ofOcimum gratissimum. Sci ResEssay. 2008; 2(5): 163-166.
32 Villines TC, Kim AS, Gore RS, Taylor AJ.Niacin: the evidence, clinical use, and future directions.Curr Atheroscler Rep. 2012; 14(1): 49-59.
33 Patil RN, Patil RY, Ahirwar B, Ahirwar D.Evaluation of antidiabetic and related actions of some Indian medicinal plants in diabetic rats.Asian Pac J Trop Med. 2011; 4(1): 20-23.
34 Moussa SA.Oxidative stress in diabetes mellitus.Romanian J Biophys. 2008; 18(3): 225-236.
35 Xu Z, Wang X, Zhou M, Ma L, Deng Y, Zhang H, Zhao A, Zhang Y, Jia W.The antidabetic activity of total lignin fromFructus Arctii against alloxan-induced diabetes in mice and rats. Phytother Res. 2008; 22(1): 97-101.
36 Hu X, Sato J, Oshida Y, Xu M, Bajotto G, Sato Y.Effect of Gosha-jinki-gan (Chinese herbal medicine) on insulin resistance in streptozotocin-induced diabetic rats.Diabetes Res Clin Pract. 2003; 59(2): 103-111.
37 Kujur RS, Singh V, Ram M, Yadava HN, Singh KK, Kumari S, Roy BK.Antidiabetic and phytochemical screening of crude extract ofStevia rebaudiana in alloxan-induced diabetic rats. Pharmacognosy Res. 2010; 2(4): 258-263.
38 Akpan OU, Effiom OE.Ocimum gratissimum impairs gut glucose absorption but enhances water absorption in streptotozocin induced diabetic rats. Gaziantep Med J. 2015; 21(1): 43-50.
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