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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (12): 1405-1412.doi: 10.3736/jcim20121212

• Original Experimental Research • Previous Articles     Next Articles

Formula-syndrome correlation study of three classical anti-jaundice formulas in inhibition of liver fibrosis induced by dimethylnitrosamine in rats

Yan-qin Bian1,2, Bing-bing Ning1,2, Hong-yan Cao1,2, Yan Lu1,2, Cheng Liu1, Gao-feng Chen1,2, Jia Liu1,3, Ping Liu1,2,3(), Ming-yu Sun1,2,3()   

  1. 1. Institute of Liver Disease, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Key Laboratory of Liver and Kidney Diseases (Ministry of Education) , Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    3. E-institute of Shanghai Municipal Education Commission, Shanghai 201203, China
  • Received:2012-07-24 Accepted:2012-08-08 Online:2012-12-20 Published:2018-12-15

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Objective: To investigate the effects of three classical anti-jaundice formulas Yinchenhao Decoction (YCHD), Yinchen Wuling San (YCWLS) and Zhizi Baipi Decoction (ZZBPD) on liver fibrosis induced by dimethylnitrosamine (DMN) in rats and explore the formula-syndrome relationship.
Methods: Forty-eight rats were weighted, and divided into five groups: normal control, DMN, YCHD, YCWLS and ZZBPD groups using stratified random method. Liver fibrosis in rats was induced by intraperitoneal injection of DMN for 4 weeks at the dosage of 10 mg/kg body weight, once per day for 3 consecutive days in each week. Each group was gavaged with distilled water (control or model group), YCHD, YCWLS and ZZBPD respectively in the last 2-week modeling period. At the end of the 4th week, rats were sacrificed and their blood sample and liver tissue were taken for detecting pathology and analyzing of hydroxyproline (Hyp) and liver function.
Results: Compared with the normal control group, DMN model group showed liver damage and liver fibrosis, with remarkably increased Hyp content (P<0.01) and abnormal liver function (P<0.01). Liver fibrosis induced by DMN in rats was improved by administration of YCHD. Compared with DMN model group, content of Hyp was decreased remarkably (P<0.01) and liver function and hepatic histology were improved significantly after 2 weeks of YCHD treatment (P<0.05 or P<0.01), while these effects were not shown in the YCWLS and ZZBPD groups. Comparison of various parameters between groups showed that content of serum total bilirubin was decreased significantly in the YCWLS group (P<0.01), and content of hepatic Hyp was decreased in the ZZBPD group.
Conclusion: Efficacy of YCHD in inhibition of liver fibrosis induced by DMN in rats is far better than YCWLS and ZZBPD. The pathogenesis of the liver fibrosis induced by DMN is mainly damp-heat. In addition, both damp and heat pathogenic factors play very important role in this model. The syndrome is highly correlated with the prescription YCHD.

Key words: liver cirrhosis,experimental, dimethylnitrosamine, compounds (TCD), rats

Table 1

Effects of three anti-jaundice formulas on body weight, liver weight, spleen weight, ratio of liver to body weight and ratio of spleen to body weight in rats with fibrosis liver (x±s)"

Group n Body weight (g) Liver weight (g) Spleen weight (g) Liver weight/
body weight (%)		 Spleen weight/
body weight (%)
A 10 318.70±25.95** 11.75±2.55** 0.66±0.07** 3.67±0.64** 0.21±0.017**
B 10 229.78±17.35 6.38±1.80 1.64±0.33 2.65±0.79 0.68±0.134
C 12 229.50±24.06 8.15±2.21* 1.61±0.18 3.54±0.69** 0.76±0.079
D 8 250.29±19.95*△ 7.47±1.62 1.61±0.17 2.85±0.61△ 0.59±0.059*△△
E 8 230.00±14.60 6.60±2.07 1.28±0.21**△△ 2.86±0.86△ 0.56±0.080**△△

Figure 1

Pathological changes in liver tissue observed by hematein and eosin staining (Light microscopy, ×200) A: Normal control group; B: Model group; C: Yinchenhao Decoction group; D: Yinchen Wuling San group; E: Zhizi Baipi Decoction group."

Figure 2

Collagen deposition in liver tissue observed by Sirius red staining (Light microscopy, ×100) A: Normal control group; B: Model group; C: Yinchenhao Decoction group; D: Yinchen Wuling San group; E: Zhizi Baipi Decoction group."

Figure 3

Effects of three anti-jaundice formulas on liver function in rats with dimethylnitrosamine-induced fibrosis *P<0.05, **P<0.01, vs B; △P<0.05, △△P<0.01, vs C. A: Normal control group; B: Model group; C: Yinchenhao Decoction group; D: Yinchen Wuling San group; E: Zhizi Baipi Decoction group. ALT: alanine aminotransferase; AST: aspartate aminotransferase; TBil: total bilirubin; ALB: albumin; GGT: gamma-glutamyl transpeptidase."

Table 2

Effects of three anti-jaundice formulas on liver Hyp content and collagen deposition in rats with dimethylnitrosamine-induced fibrosis"

Group n Hyp (x±s, μg/g fresh liver tissue) Collagen staging (n) Ridit
0 Ⅰ Ⅱ Ⅲ Ⅳ
A 10 139.82±27.11** 10 0 0 0 0 0.056±0.000**
B 10 531.30±122.57 0 0 3 2 5 0.712±0.287
C 12 394.74±72.03** 0 0 8 4 0 0.449±0.191**
D 8 450.40±93.34 0 0 3 4 1 0.593±0.240
E 8 424.32±82.11* 0 0 5 3 0 0.466±0.201*
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