冠心病痰瘀证与载脂蛋白E基因多态性关系的研究

doi:10.3736/jcim20050605

Abstract

Objective

To explore the relationship between polymorphism of apolipoprotein E (ApoE) exon 4 gene and different syndromes in patients with coronary heart disease (CHD).

Methods

Two hundred patients with CHD were divided into four groups according to syndrome differentiation, including syndrome of phlegm (PS), syndrome of blood stasis (BSS), syndrome of phlegm-blood stasis blocking (PBBS) and syndrome of non-phlegm and non-blood stasis (NPNBS). One hundred healthy volunteers were included in control group. Blood lipids were measured by routine examination. Total DNA of peripheral blood was extracted. ApoE genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. All data were analyzed by SAS software.

Results

(1) The occurrence rate of ε4 allele of ApoE in patients with CHD was 19.5%, significantly higher than 9.5% in the control group (P<0.05), and the E 3/4 genotype was especially more frequent (P<0.01). (2) The levels of total cholesterol (TC), total triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) in patients with ε4 were higher than those in patients without ε4 (P<0.01). (3) The frequencies of ε4 allele and E3/4 genotype in patients with PS were significantly higher than those in patients with BSS (P<0.05).

Conclusion

ApoE ε4 allele, especially E3/4 genotype, is the risk factor of CHD. There is a relatively close relationship between patients with ApoE ε4 allele and PS. It may be one of the main susceptible genes in CHD patients with PS.

Key words: Coronary heart disease, Apolipoprotein E, Gene polymorphism, Syndrome of phlegm and blood stasis

CLC Number:

R2-03

Tao Ouyang, Jian-nan Song, Yang Miao, Qian Lin, Xiao-hong Niu, Hong Jin, Bing Chen. Study on relationship between polymorphism of apolipoprotein E gene and syndromes of phlegm and blood stasis in patients with coronary heart disease. Journal of Chinese Integrative Medicine, 2005, 3(6): 438-442.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050605

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References 14

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Group	n	Genotype frequency [% (cases)]						Allele frequency [% (cases)]
Group	n	E 3/3	E 4/4	E 3/4	E 2/3	E 2/2	E 2/4	ε2	ε3	ε4
Control	100	66.0 (66)	2.0 (2)	14.0 (14)	17.0 (17)	0 (0)	1.0 (1)	9.0 (18)	81.5（163）	9.5 (19)
CHD	200	49.0 (98)**	2.5 (5)	33.5 (67)**	14.0 (28)	0.5 (1)	0.5 (1)	7.7 (31)	72.8 (291)	19.5 (78)*

Group	n	TC (mmol/L)	TG (mmol/L)	HDL-C (mmol/L)	LDL-C (mmol/L)	AI
E 2/2+E 2/3+E 3/3	127	4.18±1.13	1.53±1.05	1.16±0.46	2.36±0.93	2.40±1.38
E 2/4+E 3/4+E 4/4	73	4.98±1.25**	2.17±2.45*	1.09±0.42	2.90±1.00**	3.00±1.53**

Group	n	Genotype frequency [% (cases)]						Allele frequency [% (cases)]
Group	n	E 3/3	E 4/4	E 3/4	E 2/3	E 2/2	E 2/4	ε2	ε3	ε4
PS	20	30.00（6）^△	5.00（1）	55.00（11）^△	10.00（2）	0（0）	0（0）	5.00(2)	62.50(25)	32.50(13)^△
BSS	79	58.23（46）	1.27（1）	25.32（20）	13.92（11）	0（0）	1.27（1）	7.59(12)	77.85(123)	14.57(23)
PBBS	82	48.78（40）	3.66（3）	30.49（25）	15.85（13）	1.22（1）	0（0）	9.14(15)	71.95(118)	18.91(31)
NPNBS	19	31.58（6）^△	0（0）	57.89（11）*^△	10.53（2）	0（0）	0（0）	5.26(2)	65.79(25)	28.95(11)

Study on relationship between polymorphism of apolipoprotein E gene and syndromes of phlegm and blood stasis in patients with coronary heart disease

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