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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (11): 1129-1135.doi: 10.3736/jcim20081106

• Original Clinical Research • Previous Articles     Next Articles

Effects of Xuefu Zhuyu Oral Liquid on hemorheology in patients with blood-stasis syndrome due to coronary disease and their relationship with human platelet antigen-3 polymorphism

Mei Xue, Ke-Ji Chen, Xiao-juan Ma, Jian-gang Liu, Yue-rong Jiang, Yu Miao, Hui-jun Yin()   

  1. Laboratory of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
  • Received:2008-06-18 Online:2008-11-20 Published:2008-11-15

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Objective:

To investigate the effects of Xuefu Zhuyu Oral Liquid, a compound traditional Chinese herbal medicine for resolving stagnation, on hemorheology in the patients with blood-stasis syndrome due to coronary disease and their relationship with human platelet antigen-3 (HPA-3) polymorphism of membrane glycoprotein Ⅱb (GPⅡb).


Methods: 

Thirty-two patients with blood-stasis syndrome due to coronary disease were selected in this study. Blood-stasis syndrome scoring was performed and hemorheological parameters were measured in all subjects before and after Xuefu Zhuyu Qral Liquid treatment. The genotypes of GPⅡb HPA-3 were determined by TaqMan probe technology.


Results: 

The hemorheology was improved in the patients with blood-stasis syndrome due to coronary heart disease after the treatment. There were significant differences in the whole blood viscosity, deformation index of red blood cells and scores of blood-stasis syndrome between the patients carrying AC plus CC and the patients carrying AA after the treatment (P<0.05).


Conclusion: 

Xuefu Zhuyu Oral Liquid can improve clinical symptoms and hemorheology in the patients with blood-stasis syndrome due to coronary heart disease, which is related to GPⅡb HPA-3 polymorphism.

Key words: Xuefu Zhuyu Oral Liquid, coronary heart disease, blood stasis, hemorheology, platelet membrane glycoprotein H b, genes

CLC Number: 

  • R541.4

Table 1

GPⅡb HPA-3 (Baka/Bakb) polymorphism"

Amino acid pos HPA classification Personal name classification dbSNP allele dbSNP rs#cluster id
GPⅡb
Ile 843 Ser		 HPA-3a
HPA-3b		 Baka
Bakb		 A
C		 Rs5911

Table 2

GPⅡb PCR reaction system of primer detection"

Reaction component Concentration Volume (μl)
10×PCR buffer 10× 2.5
dNTP 2.5 pmol/μl 2.0
Forward primer 20 pmol/μl 0.5
Reverse primer 20 pmol/μl 0.5
Ex-Taq 0.125
H2O 18.375
Template 1.0
Total 25.0

Table 3

GPⅡb PCR reaction system of probe detection"

Reaction component Concentration Volume (μl)
2×TaqMan Master Mix 2× 5.0
Forward primer 10 pmol/μl 0.45
Reverse primer 10 pmol/μl 0.45
TaqMan FAM probe 10 pmol/μl 0.25
TaqMan TET probe 10 pmol/μl 0.25
Template DNA 10 ng/μl 2.0
ddH2O 1.6
Total 10.0

Figure 1

Rs5911 genotype pattern Abscissa: TET signal intensity; Ordinate: FAM signal intensity; NTC: No template control; Red spot: Allele C homozygote; Blue spot: Allele A homozygote; Green spot: Heterozygote."

Table 4

Whole blood viscosity on HPA-3 polymorphism locus before and after treatment (x±s, mPa·s)"

Group n 1.0 s-1 5.0s-1 10.0 s-1 35.0 s-1 120.0 s-1
AA
Before treatment 8 17.06±1.93 14.42±2.29 10.53±1.65 6.32±1.02 4.86±0.49
After treatment 8 17.19±1.61 14.83±1.24 10.07±1.45 6.75±1.41 5.03±0.57
AC+CC
Before treatment 24 17.57±2.86 15.31±2.83 11.02±1.71 6.74±0.87 4.63±0.47
After treatment 24 15.82±2.46* 13.37±2.05* 10.01±1.42* 6.62±0.58 4.61±0.39

Table 5

Hemorheology on HPA-3 polymorphism locus before and after treatment (x±s)"

Group n RBC concentration
(%)		 RBC rigidity 1 500 s-1
(%)		 Hematocrit
(%)		 Plasma viscosity
(mPa·s)		 Maximum platelet
aggregation rate (%)		 Platelet adhesion
rate (%)		 Fibrinogen (g/L)
AA
Before treatment 8 18.96±8.68 59.48±7.54 45.00±1.41 1.72±0.07 60.11±15.73 28.27±3.18 3.30±0.51
After treatment 8 17.00±5.91 60.54±6.85 44.63±1.09 1.72±0.06 57.61±13.00 28.08±5.85 3.27±0.46
AC+CC
Before treatment 24 14.09±4.36 63.25±3.58 44.19±1.71 1.73±0.04 58.03±14.88 27.90±3.06 3.16±0.36
After treatment 24 14.57±3.92 64.94±1.82* 43.40±2.11 1.72±0.04 57.47±12.03 28.90±2.89 3.14±0.33

Table 6

Scores of blood-stasis syndrome on HPA-3 polymorphism locus before and after treatment"

Group n Score of blood-stasis syndrome (x±s) P
AA
Before treatment 8 25.88±7.57 0.058
After treatment 8 20.88±6.17
AC+CC
Before treatment 24 26.33±6.67 0.000
After treatment 24 19.04±6.76
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