马齿苋总黄酮抗小鼠缺氧作用及其机制研究

doi:10.3736/jcim20050608

Abstract

Objective

To identify anti-hypoxia ingredients extracted from Portulaca oleracea and to find out the possible mechanism of its anti-hypoxia actions.

Methods

Seventy mice were randomly divided into seven groups which were untreated (normal saline), ginsenosides-treated, polysaccharide-treated, acidic components-treated, basic components-treated, alkaloids-treated and flavones-treated groups, and the ingredients of polysaccharide, acidic components, basic components, alkaloids and flavones were extracted from Portulaca olerace. The mice in each group were fed with corresponding ingredients for one week respectively. Then the survival time of mice in hypoxic conditions was observed. Another 90 mice were divided into 3 groups: untreated (normal saline), ginsenosides-treated and flavones-treated groups. The mice in each of these 3 groups were divided into 3 subgroups according to 12-, 24- and 36-hour exposure to hypoxia (10% oxygen and 90% nitrogen), respectively. After exposure to hypoxia, the red blood cell count (RBC), hemoglobin (Hb) concentration and hematocrit (HCT) in mice were determined. The plasma erythropoietin (EPO) levels of mice were detected by enzyme-linked immunosorbent assay (ELISA) and the relative values of EPO mRNA in renal tissue and pallium of mice were determined by reverse transcriptase-polymerase chain reaction (RT-PCR).

Results

The survival time of mice in hypoxic conditions in flavones-treated group was significantly longer than that in the untreated group. The RBC, Hb concentration, HCT, plasma EPO level and the relative values of EPO mRNA in renal tissue and pallium of mice were significantly higher in the flavones-treated group than those in the untreated group.

Conclusion

The anti-hypoxia ingredients extracted from Portulaca oleracea are flavones and the anti-hypoxia effects may be obtained by improving the expression level of EPO and accelerating the generations of erythrocyte and Hb.

Key words: Portulaca oleracea, Flavone, anoxia, Hemoglobins, Erythropoietin

CLC Number:

R285.5

Li-wei Dong, Wan-yin Wang, Yi-tian Yue, Min Li. Effects of flavones extracted from Portulaca oleracea on ability of hypoxia tolerance in mice and its mechanism. Journal of Chinese Integrative Medicine, 2005, 3(6): 450-454.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050608

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References 13

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Group	n	Body weight (g)	Survival time in hypoxic condition (min)
Untreated	10	23.3±4.35	37.18±9.41
Polysaccharide-treated	10	27.2±3.11	41.03±7.44
Acidic components-treated	10	26.0±1.63	37.83±12.31
Basic components-treated	10	26.1±3.48	38.12±8.34
Alkaloids-treated	10	26.4±1.90	39.23±10.29
Flavones-treated	10	23.9±0.88	50.49±10.49**^△▲
Ginsenosides-treated	10	24.7±2.76	46.63±11.02*

Group	n	RBC (×1012/L)	Hb (g/L)	HCT (L/L)	Plasma EPO (kU/L)
Untreated
12-hour exposure to hypoxia	10	7.23±0.72	122.1±14.9	0.388±0.02	0.170±0.016
24-hour exposure to hypoxia	10	7.11±0.64	117.5±13.3	0.388±0.03	0.327±0.020
36-hour exposure to hypoxia	10	6.90±0.90	127.5±5.7	0.401±0.02	0.379±0.026
Ginsenosides-treated
12-hour exposure to hypoxia	10	7.92±0.52*	133.9±10.4	0.405±0.03	0.187±0.024
24-hour exposure to hypoxia	10	7.70±0.68	126.2±12.6	0.416±0.02*	0.359±0.031*
36-hour exposure to hypoxia	10	7.79±0.47*	134.1±6.7*	0.435±0.01**	0.384±0.028
Flavones-treated
12-hour exposure to hypoxia	10	8.14±0.53*	129.3±14.2	0.423±0.01*	0.202±0.012*
24-hour exposure to hypoxia	10	7.99±0.42**	129.3±9.30*	0.423±0.01*	0.381±0.045*
36-hour exposure to hypoxia	10	7.87±0.54*	130.9±6.17	0.422±0.02*	0.426±0.040**

Group	n	EPO mRNA/β-actin
Group	n	Renal tissues	Pallium
Untreated
12-hour exposure to hypoxia	10	0.71±0.03	0.85±0.01
24-hour exposure to hypoxia	10	0.80±0.01	0.80±0.02
36-hour exposure to hypoxia	10	0.73±0.02	0.89±0.03
Ginsenosides-treated
12-hour exposure to hypoxia	10	0.93±0.08*	0.93±0.02*
24-hour exposure to hypoxia	10	1.01±0.04**	0.85±0.03*
36-hour exposure to hypoxia	10	0.78±0.02*	0.94±0.01*
Flavones-treated
12-hour exposure to hypoxia	10	0.83±0.04*	0.92±0.02*
24-hour exposure to hypoxia	10	0.88±0.02*	0.86±0.03*
36-hour exposure to hypoxia	10	0.77±0.02*	0.98±0.02**

Effects of flavones extracted from Portulaca oleracea on ability of hypoxia tolerance in mice and its mechanism

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