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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (4): 278-280.doi: 10.3736/jcim20040414

• Original Experimental Research • Previous Articles     Next Articles

Effect of soybean isoflavones on heart function of rats with adriamycin-induced heart failure

Shan-feng Ma(), Su-dong Guan, Yan Zhu   

  1. Department of Physiology, Bengbu Medical College, Bengbu,Anhui Province 233003, China
  • Received:2003-12-30 Online:2004-07-20 Published:2018-10-20

Objective: To observe the protective effect of soybean isoflavones (SI) on the heart function of the rats with adriamycin-induced heart failure.Methods: Thirty adult male SD rats were divided into 5 groups: normal control (NC) group, adriamycin (ADR) group, L-SI group, M-SI group and H-SI group. SI of 30, 60, 120 mg·kg -1·d -1was orally administered through a stomach tube once a day for 6 days in L-SI group, M-SI group and H-SI group, respectively. The other two groups were given the same amount of normal saline the same way. Then ADR of 10 mg/kg was given intraperitoneally once to copy the model of heart-failure. The MedLab-U/4c biological signal collecting system was used to record and analyze the LVSP of the rats. The pathological changes of the cardiomyocytes were observed. Results: As compared with NC group, the LVSP, ±dp/dtmax, Vpm of the ADR group were significantly lower (P<0.05 or P<0.01), but those of the H-SI group were markedly higher than those of the ADR group (P<0.01). Electron microscopic morphometry of the heart samples of the rats in ADR group revealed typical alterations, consisting an increase of collagen content, vacuolation, diminishing of the cardiomyocyte diameter, alteration of myofilaments and Z-lines of myofibers, and myofibrillar degeneration. SI of 120 mg·kg -1·d -1treatment could prevent the loss of myofibrillae and the reduction of myocyte diameter, and the degeneration of myofilaments and Z-lines were reversed by SI. Conclusion: SI of 120 mg·kg -1·d -1 treatment can relieve the toxic effect of ADR on myocardium, and also obviously improve the cardiac contractility of heart-failure rats.

Key words: soybean isoflavones, adriamycin, heart failure, congestive, rats, laboratory

CLC Number: 

  • R285.5

Tab 1

Effect of soybean isoflavones and adriamycin on heart function of rats ($\bar{x}$±s )"

Group n LVSP
(mm Hg)
+dp/dtmax
(mm Hg/s)
–dp/dtmax
(mm Hg/s)
Vpm
(/s)
NC 6 82.9±15.1 8 054.3±616.5 –4 754.8±752.5 1 676.4±243.2
ADR 6 54.9±12.5* * 3 443.8±654.3** –2 494.9±674.7** 722.0±287.7**
L-SI 6 56.6±10.7** 3 555.5±731.0** –2 563.7±359.2** 811.5±163.2**
M-SI 6 69.7±19.3 4 013.1±864.3** –3 282.2±538.9** 1 027.0±282.3**
H-SI 6 74.7±14.1 4 829.8±1 289.1** –3 649.9±821.9**△△ 1 233.5±311.4**△△

Fig 1

Ultrastructure of cardiomyocytes of the rat in different group (×10 K) A: NC group; B: ADR group; C: L-SI group; D: M-SI group; E: H-SI group"

[1] Kaplan RC, Heckbert SR, Weiss NS , et al. Postmenopausal estrogens and risk of myocardial infarction in diabetic women[J]. Diabetes Care, 1998,21(7):1117-1121
doi: 10.2337/diacare.21.7.1117 pmid: 9653605
[2] Deodato B, Altavilla D, Squadrito G , et al. Cardioprotection by the phytoestrogen genistein in experimental myocardial ischemia-reperfusion injury[J]. Br J Pharmacol, 1999,128(8):1683-1690
doi: 10.1038/sj.bjp.0702973
[3] 杜宇, 许勇 . 异黄酮的医用价值[J]. 中西医结合学报, 2003,1(4):296-300
Du Y, Xu Y . Medical value of isoflavones[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(4):296-300
[4] 金凯, 陈中军, 罗华荣 . 盐酸阿霉素对大鼠心肌损伤的分子机制研究[J]. 同济大学学报(医学版), 2002,23(3):191-194
doi: 10.3969/j.issn.1009-2501.2002.03.005
[5] 钟玲, 叶秋荣 . 刺五加对大鼠实验性缺血心肌的保护作用[J]. 中国病理生理杂志, 1995,11(4):366-369
[6] Khaidar A, Marx M, Lubec B , et al. L-arginine reduces heart collagen accumulation in the diabetic db/db mouse[J]. Circulation, 1994,90(1):479-483
doi: 10.1161/01.CIR.90.1.479
[7] 万梅, 于占久 . 内皮衍生舒张因子对缺血(缺氧)、再灌注(复氧)心肌的保护作用[J]. 生理学报, 1995,47(3):231-237
[8] 董德武, 李丽颖, 魏宝霞 . 葛根素与雌激素替代疗法对老年高血压、动脉粥样硬化患者血管内皮功能影响[J]. 中医药学报, 2002,30(1):42-43
[9] 姜桢, 郭克芳, 董苏斐 . 左旋精氨酸与氧自由基的相互作用[J]. 临床麻醉杂志, 2000,16(10):503-505
doi: 10.3969/j.issn.1004-5805.2000.10.010
[10] 闫祥华, 顾景范, 孙存普 . 大豆异黄酮对大鼠血脂和过氧化状态的影响[J]. 营养学报, 2000,22(1):31-35
[11] 郑高利, 朱寿民, 刘子贻 , 等. 大豆异黄酮的抗氧化作用[J]. 浙江医科大学学报, 1997,26(5):196-199
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