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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (10): 1118-1124.doi: 10.3736/jcim20111013

• Original Experimental Research • Previous Articles     Next Articles

Effects of different extracts of Rosa damascena on pentylenetetrazol-induced seizures in mice

Mahmoud Hosseini1(), Mahboobeh Ghasemzadeh Rahbardar1, Hamid Reza Sadeghnia2, Hassan Rakhshandeh3#br#   

  1. 1. Neuroscience Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
    2. Neuroscience Research Center and Department of Pharmacology, Department of New Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
    3. Pharmacological Research Center of Medicinal Plants and Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Received:2011-05-05 Accepted:2011-08-07 Online:2011-10-20 Published:2011-10-15
  • Contact: Hosseini Mahmoud

Objective: In the present study, the effects of aqueous, ethanolic and chloroformic extracts of Rosa damascena on pentylenetetrazol (PTZ)-induced seizures were investigated in mice.

Methods: The animals were divided into the following groups: normal saline control group, diazepam group (3 mg/kg), three aqueous extract groups (100, 500 and 1 000 mg/kg), three ethanolic extract groups (100, 500 and 1 000 mg/kg) and three chloroformic extract groups (100, 500 and 1 000 mg/kg). The extracts, normal saline or diazepam were injected intraperitoneally 30 min before PTZ injection. Latency to the first minimal clonic seizure (MCS) and generalized tonic-clonic seizure (GTCS) and the percent of mortality of rats in each group were recorded.

Results: Significant increases in both MCS and GTCS latencies were observed in all the three aqueous extract groups in comparison with the normal saline control group (P<0.05 or P<0.01). The MCS latency in the ethanolic extract (1 000 mg/kg) group and the GTCS latencies in the two ethanolic extract (500 and 1 000 mg/kg) groups were higher than those in the normal saline control group (P<0.05, P<0.01). There were no significant differences in MCS and GTCS latencies between the three chloroformic extract groups and the normal saline control group. No significant differences were seen in mortality rate following PTZ administration between the different extracts-treated mice and the control mice.

Conclusion:The results of the present study showed that R. damascena has an anticonvulsant effect in a mouse model of PTZ-induced seizures but the exact mechanism of this effect should be clarified in future studies.

Key words: Rosa damascena, Rosaceae, anticonvulsants, pentylenetetrazol, seizures, mice


Group n MCS GTCS
Normal saline control 10 40.46±1.77 49.85±2.27
Aqueous extract (100 mg/kg, i.p.) 10 56.89±5.63* 77.62±5.79**
Aqueous extract (500 mg/kg, i.p.) 10 56.80±1.54* 68.80±2.73**
Aqueous extract (1 000 mg/kg, i.p.) 10 64.80±6.71** 70.11±5.39**


Group n MCS GTCS
Normal saline control 10 40.46±1.77 49.85±2.27
Ethanolic extract (100 mg/kg, i.p.) 10 49.20±3.15 49.50±1.80
Ethanolic extract (500 mg/kg, i.p.) 10 41.70±1.64 64.66±4.48*
Ethanolic extract (1 000 mg/kg, i.p.) 10 60.60±3.14** 72.00±5.61**
[1] Libster M . Delmar’s integrative herb guide for nurses[M]. Albany: Delmar Thamson Learning, 2002, 360-370
[2] Boskabady MH, Kiani S, Rakhshandah H . Relaxant effects of Rosa damascena on guinea pig tracheal chains and its possible mechanism(s)[J]. J Ethnopharmacol, 2006,106(3):377-382
doi: 10.1016/j.jep.2006.01.013 pmid: 16504433
[3] Rakhshandah H, Boskabady MH, Mossavi Z, Gholami M, Saberi Z . The differences in the relaxant effects of different fractions of Rosa damascena on guinea pig tracheal smooth muscle[J]. Iran J Basic Med Sci, 2010,13(46):126-132
[4] Zargari A . Medicinal plants. Vol 4.5th ed[M]. Tehran: Tehran University, 1992,150
[5] Shafei MN, Rakhshandah H, Boskabady MH . Antitussive effect of Rosa damascena in guinea pigs[J]. Iran J Pharm Res, 2003,2(4):231-234
[6] Loghmani-Khouzani H, Sabzi Fini O, Safari J . Essential oil composition of Rosa damascena Mill cultivated in central Iran[J]. Sci Iran, 2007,14(4):316-319
[7] Ulusoy S, Bosgelmez-Tinaz G, Seçilmis-Canbay H . Tocopherol, carotene, phenolic contents and antibacterial properties of rose essential oil, hydrosol and absolute[J]. Curr Microbiol, 2009,59(5):554-558
doi: 10.1007/s00284-009-9475-y
[8] Yassa N, Masoomi F, Rohani Rankouhi SE, Hadjiakhoondi A . Chemical composition and antioxidant activity of the extract and essential oil of Rosa damascena from Iran, population of Guilan[J]. DARU J Pharm Sci, 2009,17(3):175-180
[9] Boskabady MH, Shafei MN, Saberi Z, Amini S . Pharmacological effects of Rosa damascena[J]. Iran J Basic Med Sci, 2011,14(4):295-307
doi: 10.1258/ebm.2011.011096 pmid: 3586833
[10] Remington JP, Wood HC . The dispensatory of the United States of America. 20th ed. Philadelphia: JB Lippincott. 1918.
[11] Ernst E, Barnes J, Abbot NC, Armstrong NC, Harkness E, Pittler MH, Stevinson C, Vogler B, White AR . The Di Bella story: emotion as opposed to fact[J]. Focus Complement Altern Ther, 1998,3(4):143-194
doi: 10.1211/fact.1998.00091
[12] Rakhshandah H, Hosseini M . Potentiation of pentobarbital hypnosis by Rosa damascena in mice[J]. Indian J Exp Biol, 2006,44(11):910-912
pmid: 17205713
[13] Gholamhoseinian A, Fallah H , Sharifi far F.Inhibitory effect of methanol extract of Rosa damascena Mill. flowers on α-glucosidase activity and postprandial hyperglycemia in normal and diabetic rats[J]. Phytomedicine, 2009,16(10):935-941
doi: 10.1016/j.phymed.2009.02.020
[14] Mahmood N, Piacente S, Pizza C, Burke A, Khan AI, Hay AJ . The anti-HIV activity and mechanisms of action of pure compounds isolated from Rosa damascena[J]. Biochem Biophys Res Commun, 1996,229(1):73-79
doi: 10.1006/bbrc.1996.1759 pmid: 8954085
[15] Nascimento Gislene GF, Locatelli J, Freitas PC, Silva Giuliana L . Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria[J]. Braz J Microbiol, 2000,31(4):247-256
[16] Aridğan BC, Baydar H, Kaya S, Demirci M , Ozbasar D , Mumcu E .Antimicrobial activity and chemical composition of some essential oils[J]. Arch Pharm Res, 2008,25(6):860-864
[17] Basim E, Basim H . Antibacterial activity of Rosa damascena essential oil[J]. Fitoterapia, 2003,74(4):394-396
doi: 10.1016/S0367-326X(03)00044-3 pmid: 12781814
[18] Biswas NR, Gupta SK, Das GK, Kumar N, Mongre PK, Haldar D, Beri S . Evaluation of ophthacare eye drops — a herbal formulation in the management of various ophthalmic disorders[J]. Phytother Res, 2001,15(7):618-620
doi: 10.1002/(ISSN)1099-1573
[19] Achuthan CR, Babu BH, Padikkala J . Antioxidant and hepatoprotective effects of Rosa damascena[J]. Pharm Biol, 2003,41(5):357-361
doi: 10.1076/phbi.41.5.357.15945
[20] Ozkan G, Sagdic O, Baydar NG, Baydar H . Note: antioxidant and antibacterial activities of Rosa damascena flower extracts[J]. Food Sci Technol Int, 2004,10(4):277-281
doi: 10.1177/1082013204045882
[21] Shahriari S, Yasa N, Mohammadirad A, Khorasani R, Abdollahi M . In vivo antioxidant potentials of Rosa damascene petal extract from Guilan, Iran, comparable to α-tocopherol[J]. Int J Pharmacol, 2007,3(2):187-190
doi: 10.3923/ijp.2007.187.190
[22] Jafari M, Zarban A, Pham S, Wang T . Rosa damascena decreased mortality in adult Drosophila[J]. J Med Food, 2008,11(1):9-13
doi: 10.1089/jmf.2007.546 pmid: 18361732
[23] Rakhshandeh H, Vahdati-Mashhadian N, Dolati K, Hosseini M . Antinociceptive effect of Rosa damascena in mice[J]. J Biol Sci, 2008,8(1):176-180
doi: 10.3923/jbs.2008.176.180
[24] Nyeem MAB, Alam MA, Awal MA, Mostofa M, Uddin SJ, Islam N, Rouf R . CNS depressant effect of the crude ethanolic extract of the flowering tops of Rosa damascena[J]. Iran J Pharmacol Ther, 2006,5(2):171-174
[25] Rakhshandah H, Shakeri MT, Ghasemzadeh MR . Comparative hypnotic effect of Rosa damascena fractions and diazepam in mice[J]. Iran J Pharm Res, 2007,6(3):193-197
[26] Kheirabadi M, Moghimi A, Rakhshande H, Rassouli MB . Evaluation of the anticonvulsant activities of Rosa damascena on the PTZ induced seizures in Wistar rats[J]. J Biol Sci, 2008,8(2):426-430
doi: 10.3923/jbs.2008.426.430
[27] Hajhashemi V, Ghannadi A, Hajiloo M . Analgesic and anti-inflammatory effects of Rosa damascena hydroalcoholic extract and its essential oil in animal models[J]. Iran J Pharm Res, 2010,9(2):163-168
[28] Sarker SD, Latif Z, Gray AI. Natural products isolation.2nd ed[M]. Totowa: Humana Press, 2006, 1-25
[29] Ebrahimzadeh Bideskan AR, Hosseini M, Mohammadpour T, Karami R, Khodamoradi M, Nemati Karimooy H, Alavi H . Effects of soy extract on pentylenetetrazol-induced seizures in ovariectomized rats[J]. J Chin Integr Med, 2011,9(6):611-618
Ebrahimzadeh Bideskan AR, Hosseini M, Mohammadpour T, Karami R, Khodamoradi M, Nemati Karimooy H, Alavi H. 大豆提取物抗戊四唑所致卵巢切除大鼠痉挛的作用[J]. 中西医结合学报, 2011,9(6):611-618
[30] Hosseini M, Sadeghnia HR, Salehabadi S, Alavi H, Gorji A . The effect of L-arginine and L-NAME on pentylenetetrazole induced seizures in ovariectomized rats, an in vivo study[J]. Seizure, 2009,18(10):695-698
doi: 10.1016/j.seizure.2009.09.008
[31] Hosseinzadeh H, Nassiri Asl M . Anticonvulsant, sedative and muscle relaxant effects of carbenoxolone in mice[J]. BMC Pharmacol, 2003,3:3
doi: 10.1186/1471-2210-3-3 pmid: 156636
[32] Ramezani R, Moghimi A, Rakhshandeh H, Ejtehadi H, Kheirabadi M . The effect of Rosa damascena essential oil on the amygdala electrical kindling seizures in rat[J]. Pak J Biol Sci, 2008,11(5):746-751
doi: 10.3923/pjbs.2008.746.751
[33] Jahangir U, Urooj S, Shah A, Ishaaq M, Habib A . A comparative clinical trial of rose petal(gul gulaab), rose hydrosol diluted(arq gulaab), and rose hydrosol(ruh gulaab) in insomnia[J]. Internet J Neurol, 2009,11(2):1
[34] Rakotonirina VS, Bum EN, Rakotonirina A, Bopelet M . Sedative properties of the decoction of the rhizome of Cyperus articulatus[J]. Fitoterapia, 2001,72(1):22-29
doi: 10.1016/S0367-326X(00)00243-4 pmid: 11163936
[35] Rocha FF, Lapa AJ, De Lima TC . Evaluation of the anxiolytic-like effects of Cecropia glazioui Sneth in mice[J]. Pharmacol Biochem Behav, 2002,71(1-2):183-190
doi: 10.1016/S0091-3057(01)00695-5 pmid: 2011812521
[36] Sugiyama K, Muteki T, Kano T . The Japanese herbal medicine “Saiko-Keishi-To” activates GABAA receptors of rat sensory neurons in culture[J]. Neurosci Lett, 1996,216(3):147-150
doi: 10.1016/0304-3940(96)13000-7
[37] Kim HS, Jang CG, Oh KW, Oh S, Rheu HM, Rhee GS, Seong YH, Park WK . Effects of ginseng total saponin on morphine-induced hyperactivity and conditioned place preference in mice[J]. J Ethnopharmacol, 1998,60(1):33-42
doi: 10.1016/S0378-8741(97)00131-1
[38] Kim HS, Jang CG, Park WK, Oh KW, Rheu HM, Cho DH, Oh S . Blockade by ginseng total saponin of methamphetamine-induced hyperactivity and conditioned place preference in mice[J]. Gen Pharmacol, 1996,27(2):199-204
doi: 10.1016/0306-3623(95)02023-3
[39] Dallmeier K, Carlini EA . Anesthetic, hypothermic, myorelaxant and anticonvulsant effects of synthetic eugenol derivatives and natural analogues[J]. Pharmacology, 1981,22(2):113-127
doi: 10.1159/000137479
[40] Guénette SA, Hélie P, Beaudry F, Vachon P . Eugenol for anesthesia of African clawed frogs(Xenopus laevis)[J]. Vet Anaesth Analg, 2007,34(3):164-170
doi: 10.1111/j.1467-2995.2006.00316.x pmid: 17444929
[41] Hikasa Y, Takase K, Ogasawara T, Ogasawara S . Anesthesia and recovery with tricaine methanesulfonate, eugenol and thiopental sodium in the carp, Cyprinus carpio[J]. Nihon Juigaku Zasshi, 1986,48(2):341-351
doi: 10.1292/jvms1939.48.341
[42] de Almeida ER, Rafael KR, Couto GB, Ishigami AB . Anxiolytic and anticonvulsant effects on mice of flavonoids, linalool, and α-tocopherol presents in the extract of leaves of Cissus sicyoides L.( Vitaceae). J Biomed Biotechnol. 2009: 274740. [Epub 2009 Mar 12].
[43] Elisabetsky E, Brum LF, Souza DO . Anticonvulsant properties of linalool in glutamate-related seizure models[J]. Phytomedicine, 1999,6(2):107-113
doi: 10.1016/S0944-7113(99)80044-0 pmid: 10374249
[44] Schiber A, Mihalev K, Berardini N, Mollov P, Carle R . Flavonol glycosides from distilled petals of Rosa damascena Mill[J]. Z Naturforsch C, 2005,60(5-6):379-384
doi: 10.1515/znc-2005-5-602 pmid: 2016042335
[45] Tabrizi H, Mortazavi SA, Kamalinejad M . An in vitro evaluation of various Rosa damascena flower extracts as a natural antisolar agent[J]. Int J Cosmet Sci, 2003,25(6):259-265
doi: 10.1111/j.1467-2494.2003.00189.x pmid: 18494908
[46] Joshi D, Naidu PS, Singh A, Kulkarni SK . Protective effect of quercetin on alcohol abstinence-induced anxiety and convulsions[J]. J Med Food, 2005,8(3):392-396
doi: 10.1089/jmf.2005.8.392 pmid: 16176153
[47] Manigauha A, Patel S, Monga J, Ali H . Evaluation of anticonvulsant activity of Pongamia pinnata Linn in experimental animals[J]. Int J PharmTech Res, 2009,1(4):1119-1121
[48] Kumar S, Singh RK, Singh AK, Gupta MK . Anticonvulsant activity of ethanolic extract of Solanum melongena Linn. roots[J]. Int J Pharm Sci Res, 2010,1(12):170-174
[49] Kumar N, Bhandari P, Singh B, Gupta AP, Kaul VK . Reversed phase-HPLC for rapid determination of polyphenols in flowers of rose species[J]. J Sep Sci, 2008,31(2):262-267
doi: 10.1002/jssc.200700372 pmid: 18172921
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