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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (12): 1233-1237.doi: 10.3736/jcim20081205

• Original Clinical Research • Previous Articles     Next Articles

Relationship between plasma protein expression profiles and states of Zang-Fu organs in patients with phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis

Jian-nan Song1(), Jun-lian Liu1, Xiang-zhong Fang2, Yuan-hui Hu3, Yan Lei4, Xiao-hong Niu1, Gang Wu5, Bao-sheng Chen5, Ya-luan Ma1, Bing Chen1, Hong jin1   

  1. 1. Research Center of Basic Chinese Medicine, Institute of Basic Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700, China
    2. Department of Probability and Statistics, School of Mathematical Sciences, Peking University, Beijing 100871, China
    3. Department of Cardiovascular Diseases, Guang'anmen Hospital,China Academy of Chinese Medical Sciences, Beijing 100053,China
    4. Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
    5. Department of Biochemistry, Institute of Basic Medical Sciences, China Academy of Medical Sciences, Beijing 100730, China
  • Received:2008-06-19 Online:2008-12-20 Published:2008-12-15

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Objective: To investigate the relationship between the plasma biomarker proteins and the states of Zang-Fu organs in patients with phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis.
Methods: The states of Zang-Fu organs in 146 patients with perlipidemia and atherosclerosis were diagnosed by syndrome differentiation of traditional Chinese medicine. The plasma proteins from these patients were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Differential protein profiling was established by Image Master 6.0 software, and the differential proteins were analyzed by quadrupole time of flight mass spectrometry (Q-TOF-MS). The association between the plasma biomarker proteins and the states of Zang-Fu organs was analyzed by graphical models.
Results: The biomarker proteins such as fibrinogen gamma chain, albumin and apolipoprotein AI (precursor) in discrimination of the patients with phlegm syndrome from phlegm accumulating with stagnation syndrome were correlated with the deficiency of kidney-qi, heart-qi and spleen-qi. Among the four biomarker proteins in discrimination of the patients with phlegm syndrome from blood stagnation syndrome, albumin, adrenomedullin binding protein (precursor) and haptoglobin (precursor) were correlated with the deficiency of kidney-qi and heart-qi, but complement component C4 was independent of the deficient Zang-Fu organs. The biomarker albumin was associated with the deficiency of kidney-qi, heart-qi and spleen-qi, and adrenomedullin binding protein (precursor) was correlated with the deficiency of spleen-qi in discrimination of the patients with blood stagnation syndrome from phlegm accumulating with stagnation syndrome. As the potential biomarker proteins in discrimination of the patients with non-phlegm and non-stagnation syndrome from phlegm accumulating with stagnation syndrome, the fibrinogen beta chain was related with the deficiency of kidney-qi, and apolipoprotein AI (precursor) was correlated with both the deficiency of kidney-qi and heart-qi.
Conclusion: There exists inherent correlation between the states of Zang-Fu organs and the plasma probable biomarker proteins in the patients with different phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis.

Key words: hyperlipidemias, atherosclerosis, phlegm syndrome, blood stagnation, plasma proteins, Zang-Fu organs

CLC Number: 

  • R589.21

Table 1

Frequency of the states of Zang-Fu organs in the patients with phlegm or blood stagnation syndromes"

Sydrome type n Deficiency of heart-qi Deficiency of kidney-qi Deficiency of spleen-qi
Phlegm 32 20 14 12
Blood stagnation 38 28 22 0
Phlegm accumulating with stagnation 41 22 25 19
Non-phlegm and non-stagnation 35 16 16 8

Figure 1

Relationship between the plasma biomarker proteins and the states of Zang-Fu organs in the patients with phlegm or blood stagnation syndromes A: Phlegm syndrome; B: Blood stagnation syndrome; C: Phlegm accumulating with stagnation sydrome; D: Non-phlegm and non-stagnation syndrome."

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