Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (1): 44-46.doi: 10.3736/jcim20030118

Previous Articles     Next Articles

Effect of sophoricoside on histomorphology of bone in ovariectomized rats

Ning Du(), Yong Xu, Wei-zhen Chen, Feng-hua Zhang   

  1. Department of Orthopaedics and Traumatology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China
  • Received:2003-03-15 Online:2003-03-20 Published:2018-10-19


To evaluate the potential preventive effect of sophoricoside on bone loss in ovariectomized rats.


Female SD rats (n=50, 6 months old) were either sham-operated (SHAM group, n=10) or ovariectomized (n=40). Three days after operation, ovariectomized rats were randomly assigned to groups as follows: 10 received sophoricoside 4 mg·kg -1·d -1(SL group), 10 received sophoricoside 8 mg·kg -1·d -1 (SM group), 10 received sophoricoside 16 mg·kg -1·d -1 (SH group) and 10 were untreated (OVX group). After sophoricoside were given orally for one month, the histomorphometric parameters in the secondary spongiosa of proximal tibia and lumbar vertebrae were examined.


Compared with OVX group, SM and SH two kinds of treatment caused 15.28% and 22.81% increment in percent trabecular area (BV/TV,%) in the secondary spongiosa of proximal tibia, 14.23% and 21.2% increment in lumbar vertebrae. Accordingly in these two groups, there was a significant decrease in trabecular separation (FLAW, μm), but increment in trabecular width (Tb.Th, μm) and conjunction points (JOINT). But in SL group, the preventive effect was not observed.


sophoricoside can be efficient in preventing ovariectomy-induced bone loss in rats.

Key words: sophoricoside, osteoporosis, ovariectomized rats, bone histomorphometry

CLC Number: 

  • R681.4


BV/TV,% Tb.Th,μm FLAW,μm JOINT
SHAM 62.28±3.46* 166.69±10.67* 132.700±6.36* 3.6±0.84*
OVX 29.31±2.71 96.77±7.39 196.450±22.23 1.2±0.78
SL 35.70±11.15 100.58±11.37 186.623±34.12 2.4± 1.84
SM 44.59±9.60* 122.29±10.18* 143.840±9.00* 2.6±0.84*
SH 52.12±18.67* 128.02±14.63* 167.330±18.70* 3.0±1.30*


BV/TV,% Tb.Th,μm FLAW,μm JOINT
SHAM 56.53±7.40* 188.62±11.91* 163.30±19.27* 3.8±1.40*
OVX 27.32±10.13 78.60±8.60 253.74±31.29 0.8±0.78
SL 36.67±7.29 82.70±10.90 222.73±45.79 1.4±0.52
SM 41.55±4.71* 111.25±5.90* 204.26±15.16* 3.4±0.52*
SH 48.52±7.57* 126.85±9.54* 164.45±53.42* 3.4±0.84*


组别 子宫干重
SHAM 19.15 ±1.12*
OVX 11.59±2.26
SL 8.07±1.90*
SM 8.03±1.45*
SH 8.08±2.31*
[1] 秦岭, 张戈译 . 美国国家卫生院有关骨质疏松症的预防、诊断和治疗的共识文件[J]. 中国骨质疏松杂志, 2002,8(1):90-93
doi: 10.3969/j.issn.1006-7108.2002.02.029
[2] 朴俊红, 庞莲萍, 刘忠厚 , 等. 中国人口状况及原发性骨质疏松症诊断标准和发病率[J]. 中国骨质疏松杂志, 2002,8(1):1-7
[3] Thompson DD, Simmons HA, Pirie CM , et al. FDA guidelines and animal models for osteoporosis[J]. Bone, 1995,17(4 Suppl):125-133
doi: 10.1016/8756-3282(95)00285-L pmid: 8579908
[4] Fanti O, Faugere MC, Gang Z , et al. Systemic administration of genistein partially prevents bone loss in ovariectomized rats via a nonestrogen-like mechanism[J]. Am J Clin Nutr, 1996,68(Suppl):1517-1522
[5] Ishida H, Uesugi T, Hirai K , et al. Preventive effects of the plant isoflavone, daidzin and genistein on bone loss in ovariectomized rats fed a calcium-deficient diet[J]. Biol Pharm Bull, 1998,11(1):62-66
[6] Ishimi Y, Arai N, Wang X , et al. Difference in effective dosage of genistein on bone and uterus in ovariectomized mice[J]. Biochem Biophys Res Commun, 2000,274(3):697-701
doi: 10.1006/bbrc.2000.3175 pmid: 10924338
[1] Joon-ho Lee, Yuan-ji Wei, Zhong-yan Zhou, Yu-ming Hou, Cheng-long Wang, Li-bo Wang, Hong-jin Wu, Yu Zhang, Wei-wei Dai. Efficacy of the herbal pair, Radix Achyranthis Bidentatae and Eucommiae Cortex, in preventing glucocorticoid-induced osteoporosis in the zebrafish model. Journal of Integrative Medicine, 2022, 20(1): 83-90.
[2] Syed Nasir Abbas Bukhari, Fahad Hussain, Hnin Ei Thu, Zahid Hussain. Synergistic effects of combined therapy of curcumin and Fructus Ligustri Lucidi for treatment of osteoporosis: cellular and molecular evidence of enhanced bone formation. Journal of Integrative Medicine, 2019, 17(1): 38-45.
[3] Lei Liu, Yuan-hui Guo , Hai-liang Xin , Yan Nie , Ting Han, Lu-ping Qin , Oiao-yan Zhang. Antiosteoporotic effects of benzylbenzoate glucosides from Curculigo orchioides in ovariectomized rats. Journal of Chinese Integrative Medicine, 2012, 10(12): 1419-1426.
[4] Gui-lin Ouyang, Xiao-hui Feng, Lian-bo Xiao, Zheng Huang, Qing Xia, Da-peng Han, Fei Zhu . Effects of Chinese herbal medicine Qianggu Capsule on patients with rheumatoid arthritis-induced osteoporosis: a report of 82 cases. Journal of Chinese Integrative Medicine, 2012, 10(12): 1394-1399.
[5] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278.
[6] Dong-tao Li, Jian Wang , Hong-yang Jiang , Feng-lei Shi , Fu-yu Li , Ji-hong Liu , Yong-mei Cheng , Nan Yan , Ai-hua Hu, Mei-zeng Zhang , Jie Li, Ling-bo Wei , Rong-qin Jiang. Quantitative evaluation of the degrees of traditional Chinese medicine qualitative syndromes of osteoporosis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1254-1262.
[7] Lei Bao, Shi-en Zou, Shao-fen Zhang. Dose-dependent effects of daidzein in regulating bone formation through estrogen receptors and peroxisome proliferator-activated receptor γ. Journal of Chinese Integrative Medicine, 2011, 9(2): 165-172.
[8] Zhi-ming Huang, Gui-ling Ouyang, Lian-bao Xiao, Ning-li Li, Hua-li Gao, Yong He, Zheng Huang, Xin-xing Huang. Effects of Drynaria total flavonoids on apoptosis of osteoblasts mediated by tumor necrosis factor-α. Journal of Chinese Integrative Medicine, 2011, 9(2): 173-178.
[9] Li Dong-tao, Li Fu-yu, Wang Jian, Liu Ji-hong, Yan Nan, Cheng Yong-mei, Hu Ai-hua, Jiang Hong-yang, Shi Feng-lei, Zhang Mei-zeng, Li Jie, Wei Ling-bo, Jiang Rong-qin. A study of diagnostic criteria for traditional Chinese medicine syndromes in osteoporosis. Journal of Chinese Integrative Medicine, 2011, 9(12): 1326-1332.
[10] Praveen Srikanta, Shivaprasad H. Nagarajappa, Gollapalle L. Viswanatha, Mukund Handral, Rajesh Subbanna, Rangappa Srinath, Ganesh Hiremath. Anti-osteoporotic activity of methanolic extract of an Indian herbal formula NR/CAL/06 in ovariectomized rats. Journal of Chinese Integrative Medicine, 2011, 9(10): 1125-1132.
[11] Xin-xiang Wang , Yun-ling Zhang, Qi-fu Huang. Discussion on the main pathogenesis in traditional Chinese medicine and etiology about primary osteoporosis. Journal of Chinese Integrative Medicine, 2010, 8(12): 1119-1123.
[12] WEI Peng, JIAO Lei, QIN Lu-ping, YAN Fei, HAN Ting, ZHANG Qiao-yan. Effects of berberine on differentiation and bone resorption of osteoclasts derived from rat bone marrow cells. Journal of Chinese Integrative Medicine, 2009, 7(4): 342-348.
[13] GD Zhao, KJ Gao, YM Tian, ZW Ye, W Li, HP Song, ZY Cai. Experimental study of Gukangling Decoction combined with technetium [99Tc] methylene diphosphonate injection in treating osteoporotic rabbits. Journal of Chinese Integrative Medicine, 2008, 6(3): 298-303.
[14] Hua Nian​, Ling-ling Xu​, Ming-hua Ma​, Lu-ping Qin​, Han-chen Zheng​, Qiao-yan Zhang​. Prevention of bone loss by aqueous extract of Epimedii sagittatum in an ovariectomized rat model of osteoporosis. Journal of Chinese Integrative Medicine, 2006, 4(6): 628-633.
[15] Qiang Wang​, Kun-zheng Wang​, Guang-ye Wang​. Effects of soybean isoflavones on expression levels of osteoprotegerin and osteoprotegerin ligand mRNAs in bone tissues of ovariectomized rats. Journal of Chinese Integrative Medicine, 2006, 4(3): 303-306.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228