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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (3): 211-214.doi: 10.3736/jcim20030320

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Effect of salvianolate on migration of human vascular endothelial cells

Jie Xu, Wei-hu Fan   

  1. Department of Cardiology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2003-05-19 Online:2003-09-20 Published:2003-09-20

Objective

To study the effect of salvianolate on the migration of endothelial cells induced by monocytes.


Methods

Transwell-boydom system was used to test the migration of endothelial cells induced by monocytes. Enzyme-linked immunosorbent assay (ELISA) and RT-polymerase chain reaction (RT-PCR) method were used to determine the effect of salvianolate on the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)and their mRNA of monocytes.


Results

The migration of endothelial cells induced by monocytes was facilitated by salvianolate, compared with the control. After the monocytes were treated by salvianolate for 24 hours, the expression of mRNA of VEGF and bFGF was significantly up-regulated, and the expression of VEGF and bFGF was increased.


Conclusion

The facilitation of salvianolate on the migration of endothelial cells induced by monocytes was observed. Salvianolate stimulates the expression of VEGF and bFGF and their mRNA of monocytes, and salvianolate may have the role of inducing migration of endothelial cells by working on these two factors.

Key words: salvianolate, monocytes, endothelium, cell movement, endothelial cell-derived growth factors, basic fibroblast growth factor

CLC Number: 

  • R286.2

"

组别 n 迁移数 迁移促进率(%)
生理盐水对照组 8 48±6
0.25 g/L丹参多酚酸盐组 8 60±9 25
0.5 g/L丹参多酚酸盐组 8 69±11* 44
1 g/L丹参多酚酸盐组 8 75±13* 56

"

组别 n VEGF bFGF
生理盐水对照组 8 37.9±5.2 4.6±1.2
0.25 g/L丹参多酚酸盐组 8 53.5±6.3 8.6±1.9
0.5 g/L丹参多酚酸盐组 8 59.4±6.9* 12.3±2.4*
1 g/L丹参多酚酸盐组 8 66.3±7.1** 13.4±2.8*

"

[1] Leung DW, Cachianes G, Kuang WJ , et al. Vascular endothelial growth factor is a secreted angiogenic mitogen[J]. Science, 1989,246(4935):1306-1309
doi: 10.1126/science.2479986
[2] Ferrara N, Houck KA, Jakeman LB , et al. The vascular endothelial growth factor family of polypeptides[J]. J Cell Biochem, 1991,47(3):211-218
doi: 10.1002/(ISSN)1097-4644
[3] Dvorak HF, Brown LF, Detmar M , et al. Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis[J]. Am J Pathol, 1995,146(5):1029-1039
[4] Gospodarowicz D, Ferrara N, Schweigerer L , et al. Structural characterization and biological functions of fibroblast growth factor[J]. Endocrine Rev, 1987,8(2):95-114
doi: 10.1210/edrv-8-2-95 pmid: 2440668
[5] 吴永全, 袁良俊, 胡大一 . 生长因子在PTCA术后再狭窄形成中的作用[J]. 国外医学·心血管病分册, 1999,26(2):75-77
[6] Montesano R, Vassalli JD, Baird A , et al. Basic fibroblast growth factor induces angiogenesis in vitro[J]. Proc Natl Acad Sci U S A, 1986,83(19):7297-7301
doi: 10.1073/pnas.83.19.7297 pmid: 2429303
[7] Ferrara N, Davis-Smyth T . The biology of vascular endothelial growth factor[J]. Endocr Rev, 1997,18(1):4-25
doi: 10.1007/978-1-4613-0389-3_7
[8] Eckenstein FP . Fibroblast growth factors in the nervous system[J]. J Neurobiol, 1994,25(11):1467-1480
doi: 10.1002/neu.480251112 pmid: 7852998
[9] 徐理纳 . 血瘀证与活血化瘀(第1版)[M]. 北京: 学苑出版社, 1990,( 第1版):223-226
[10] 邹正午, 徐理纳, 田金英 . 迷迭香酸抗血栓和抗血小板聚集的作用[J]. 药学学报, 1993,28(4):241-245
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